- Transgenic Mouse, ICSI and IVF Core The Transgenic Mouse Core was established during Phase I of the IBR COBRE based on novel transposase-mediated gene delivery and targeting technology developed at our institute by one of our COBRE investigators. During the first four years, the Core made significant progress towards the goal of becoming independently supported within fifteen years. We have accumulated a large array of equipment for micromanipulation of mouse gametes and embryo culture, and have recruited an experienced manager to head the Core laboratory. Before establishing the Core, we consulted with two established transgenic cores, one of which was COBRE supported at the University of Nevada. The IBR Core has made excellent progress, having produced 36 transgenic mouse lines, and developed new services that the COBRE investigators required that the Core was well suited to provide. The Core continued the development of novel gene delivery technology, including making significant progress on targeted gene insertion. Thus, the Core has contributed to the enhancement of the institution's research infrastructure by providing the COBRE investigators with transgenic mice and other services, research development, and training the next generation of scientists. In this Phase II application, we propose to enhance and transform the Core by expanding the services it provides through: (1) continued research and development to increase the efficiency of transgenic mouse production, and (2) a major renovation of animal and laboratory space that is being funded by the university as institutional support of the IBR COBRE. The Core will directly support four new COBRE Project Leaders. Because of this expansion, we have more accurately named it: the Transgenic Mouse, ICSI and IVF (TMII) Core. Dr. Stefan Moisyadi, the leader of the group that developed the new technology for gene insertion, and who was a Phase I junior investigator that graduated to independence, will become the TMII Core Director. These goals will be achieved through the following Specific Aims:
Specific Aim 1. Improve the TMII Core through expansion of services, infrastructure development and renovation of the core laboratories. The Core will continue to provide transgenic mice using conventional technologies while incorporating rapidly emerging new technologies in the field, both from our own group and from others. We will also add new services such as ICSI and IVF to Project Leaders in Phase II. A $4 million renovation to the UH Manoa vivarium will allow transgenic mice to be produced much more efficiently. This will be an important step towards the development of our independence in Phase II.
Specific Aim 2. Enhance and transform the IBR TMII Core through novel technology developed in Phase I of the IBR COBRE and the continued development of gene delivery systems. The TMII Core will continue to develop gene insertion technology during Phase II under the direction of Dr. Moisyadi.

Public Health Relevance

- Transgenic Mouse, ICSI and IVF Core The TMII Core will generate mice with genes inserted into them that are important for medical research. The scientists at our institute have developed unique methods for producing these mice that are more efficient than at other similar cores. Moreover, this Core is the only facility in the State of Hawaii that can produce genetically altered mice.

Agency
National Institute of Health (NIH)
Type
Exploratory Grants (P20)
Project #
2P20GM103457-06A1
Application #
8737526
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Hawaii
Department
Type
DUNS #
City
Honolulu
State
HI
Country
United States
Zip Code
96822
Kono, Kanako; Tamashiro, Dana Ann A; Alarcon, Vernadeth B (2014) Inhibition of RHO-ROCK signaling enhances ICM and suppresses TE characteristics through activation of Hippo signaling in the mouse blastocyst. Dev Biol 394:142-55
Collier, Abby C; Yamauchi, Yasuhiro; Sato, Brittany L M et al. (2014) UDP-glucuronosyltransferase 1a enzymes are present and active in the mouse blastocyst. Drug Metab Dispos 42:1921-5
Comptour, Aurélie; Moretti, Charlotte; Serrentino, Maria-Elisabetta et al. (2014) SSTY proteins co-localize with the post-meiotic sex chromatin and interact with regulators of its expression. FEBS J 281:1571-84
Ching, Travers; Huang, Sijia; Garmire, Lana X (2014) Power analysis and sample size estimation for RNA-Seq differential expression. RNA 20:1684-96
Yamauchi, Yasuhiro; Riel, Jonathan M; Stoytcheva, Zoia et al. (2014) Two Y genes can replace the entire Y chromosome for assisted reproduction in the mouse. Science 343:69-72
Wu, Zhenfang; Xu, Zhiqian; Zou, Xian et al. (2013) Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer. Transgenic Res 22:1107-18
Riel, Jonathan M; Yamauchi, Yasuhiro; Sugawara, Atsushi et al. (2013) Deficiency of the multi-copy mouse Y gene Sly causes sperm DNA damage and abnormal chromatin packaging. J Cell Sci 126:803-13
Hirate, Yoshikazu; Hirahara, Shino; Inoue, Ken-Ichi et al. (2013) Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Curr Biol 23:1181-94
Gawecka, Joanna E; Marh, Joel; Ortega, Michael et al. (2013) Mouse zygotes respond to severe sperm DNA damage by delaying paternal DNA replication and embryonic development. PLoS One 8:e56385
Jaremko, Kate Lynn; Marikawa, Yusuke (2013) Regulation of developmental competence and commitment towards the definitive endoderm lineage in human embryonic stem cells. Stem Cell Res 10:489-502

Showing the most recent 10 out of 11 publications