- Project 3 Obesity has become a global pandemic. In the Unites States alone, according the CDC data 2008, 33.8% adults were obese and 68% adults were obese or overweight. The State of Hawaii has multiple ethnic groups, in which Native Hawaiian and Pacific Islanders (NHPI) have a daunting obesity rate of 49.3%, highest of any racial/ethnic group. The effects of maternal obesity are trans-generational, in that maternal obesity increases the likelihood of neonatal obesity, and the neonatal obesity is likely to persist in the adulthood. Additionally, obesity is correlated with various diseases including cancers in adults. Epidemiological studies have provided the link that maternal obesity can lead to increased cancer risks in progenies in later adult life. However, due to ethical and practical reasons in human studies, direct evidence is lacking. Here we propose a case control study with multi-ethnic groups (Asian, Native Hawaiian and Pacific Islanders and Caucasians) to investigate the molecular links between maternal obesity and offspring cancer risks, through the integration of transcriptome and methylome of the cord blood stem cells. The de-identified cord blood tissue will be collected through the Hawaii Biorepository (HiBR). Specifically, we will select the hematopoietic stem cells, and perform RNA-Seq transcriptome and methylome experiments, respectively. These data will be compared to the transcriptome data, methylome data, as well as the integrated transcriptome and methylome data, from The Cancer Genome Atlas. Through such comparison, we hope to identify the cancer-like modules that signify the difference between cord blood stem cells associated with obese mothers vs. those of mothers with normal BMI. This study will hopefully help provide effective strategies to prevent obesity and cancers to occur even before the child is born. It will also help to provide remedial strategies to solve the outstanding health disparity issue in the State of Hawaii.

Public Health Relevance

- Project 3 The goal of this proposal is to investigate the molecular links between maternal obesity and offspring cancer risks, through the integration of transcriptome and methylome of the cord blood stem cells obtained from a multi-ethnic case control study, in comparison with the data of the Cancer Genome Atlas (TCGA).

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
2P20GM103457-06A1
Application #
8737529
Study Section
Special Emphasis Panel (ZGM1)
Project Start
2014-07-01
Project End
2019-06-30
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Hawaii
Department
Type
DUNS #
City
Honolulu
State
HI
Country
United States
Zip Code
96822
Ward, W Steven (2017) Eight tests for sperm DNA fragmentation and their roles in the clinic. Transl Androl Urol 6:S468-S470
Ulu, Ferhat; Kim, Sung-Min; Yokoyama, Toshifumi et al. (2017) Dose-dependent functions of fibroblast growth factor 9 regulate the fate of murine XY primordial germ cells. Biol Reprod 96:122-133
Yuan, Chloe J; Marikawa, Yusuke (2017) Developmental toxicity assessment of common excipients using a stem cell-based in vitro morphogenesis model. Food Chem Toxicol 109:376-385
Pomozi, Viola; Brampton, Christopher; van de Wetering, Koen et al. (2017) Pyrophosphate Supplementation Prevents Chronic and Acute Calcification in ABCC6-Deficient Mice. Am J Pathol 187:1258-1272
Polgar, Noemi; Fogelgren, Ben (2017) Regulation of Cell Polarity by Exocyst-Mediated Trafficking. Cold Spring Harb Perspect Biol :
Garmire, Lana X; Gliske, Stephen; Nguyen, Quynh C et al. (2017) THE TRAINING OF NEXT GENERATION DATA SCIENTISTS IN BIOMEDICINE. Pac Symp Biocomput 22:640-645
Yang, Jennifer; Tanaka, Yoshiaki; Seay, Montrell et al. (2017) Single cell transcriptomics reveals unanticipated features of early hematopoietic precursors. Nucleic Acids Res 45:1281-1296
Elston, Marlee; Urschitz, Johann (2017) Transposase-mediated gene modulation in the placenta. Placenta 59 Suppl 1:S32-S36
Zhu, Xun; Ching, Travers; Pan, Xinghua et al. (2017) Detecting heterogeneity in single-cell RNA-Seq data by non-negative matrix factorization. PeerJ 5:e2888
Feng, Nannan; Wang, Yu; Zheng, Min et al. (2017) Genome-wide analysis of DNA methylation and their associations with long noncoding RNA/mRNA expression in non-small-cell lung cancer. Epigenomics :

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