The major goal of this COBRE application is to foster the development of COBRE investigators in the Center of Pediatric Research and help them to succeed in their research programs. This will be carried out by a multidisciplinary group of researchers at Sanford Research/USD who are dedicated to basic and translational biomedical research. To promote the growth of the only multidisciplinary training program for pediatric basic and translational studies in South Dakota, the Sanford Research/USD will establish infrastructures to provide essential and centralized laboratory service to COBRE project leaders. The Molecular Genetics Core facility is a laboratory in the Children's Health Research Center offering efficient, high quality and affordable molecular genetics services for COBRE project leaders and other researchers in Sanford Research/USD. The state-of-the-art, high-throughput equipment in the laboratory will enable a wide range of molecular genetics assays, including extraction of nucleic acids from tissues and cells, quantitative and qualitative analysis of nucleic acid samples, gene expression analysis and training. The Molecular Genetics Core facility will provide personalized services for COBRE project leaders from experimental design to assay execution and data analysis. A plan is developed for a sustainable Molecular Genetics Core facility to serve the needs of COBRE project leaders and other researchers in Sanford Research/USD. By providing the funding, resources and core lab services, Sanford Research/USD will promote the advancement of this core group of scientists to build a stronger pediatric research program, and ultimately increase the number of NlH-funded biomedical researchers in South Dakota.

Public Health Relevance

The Molecular Genetics Core will provide services and expertise for molecular genetic assays and techniques. These services are critical for advancing studies involving elucidation of gene function in health and disease.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Exploratory Grants (P20)
Project #
Application #
Study Section
Special Emphasis Panel (ZGM1-TWD-B (CB))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Sanford Research/Usd
Sioux Falls
United States
Zip Code
White, Katherine A; Swier, Vicki J; Cain, Jacob T et al. (2018) A porcine model of neurofibromatosis type 1 that mimics the human disease. JCI Insight 3:
Anderson, Ruthellen H; Lensing, Cody J; Forred, Benjamin J et al. (2018) Differentiating Antiproliferative and Chemopreventive Modes of Activity for Electron-Deficient Aryl Isothiocyanates against Human MCF-7 Cells. ChemMedChem 13:1695-1710
Johnson, Tyler B; Mechels, Keegan; Anderson, Ruth Ellen et al. (2018) Characterization of a recurrent missense mutation in the forkhead DNA-binding domain of FOXP1. Sci Rep 8:16161
Brudvig, J J; Cain, J T; Sears, R M et al. (2018) MARCKS regulates neuritogenesis and interacts with a CDC42 signaling network. Sci Rep 8:13278
Roux, Kyle J; Kim, Dae In; Burke, Brian et al. (2018) BioID: A Screen for Protein-Protein Interactions. Curr Protoc Protein Sci 91:19.23.1-19.23.15
Hussain, Sajjad; Bedekovics, Tibor; Liu, Qiuying et al. (2018) UCH-L1 bypasses mTOR to promote protein biosynthesis and is required for MYC-driven lymphomagenesis in mice. Blood 132:2564-2574
Brudvig, J J; Cain, J T; Schmidt-Grimminger, G G et al. (2018) MARCKS Is Necessary for Netrin-DCC Signaling and Corpus Callosum Formation. Mol Neurobiol 55:8388-8402
Anderson, Ruthellen H; Kerkvliet, Jason G; Otta, Jaelin J et al. (2018) Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking. Stem Cell Res 33:95-99
Lucido, Christopher T; Callejas-Valera, Juan L; Colbert, Paul L et al. (2018) ?2-Adrenergic receptor modulates mitochondrial metabolism and disease progression in recurrent/metastatic HPV(+) HNSCC. Oncogenesis 7:81
McKenzie, Casey W; Preston, Claudia C; Finn, Rozzy et al. (2018) Strain-specific differences in brain gene expression in a hydrocephalic mouse model with motile cilia dysfunction. Sci Rep 8:13370

Showing the most recent 10 out of 59 publications