The Yeast 2-Hybrid Core will provide support and expertise in detection of novel protein-protein interactions through Yeast 2-Hybrid screening for the COBRE research Project Leaders, as well as other faculty both at Sanford Research/USD and at regional research institutes. The core will provide several different approaches for Yeast 2-Hybrid screening, allowing for the design of a unique, customized method for screening protein-protein interactions that best meet the needs of each individual project. By centralizing these Yeast 2-Hybrid services, the core will minimize labor and cost to individual laboratories interested in performing screens as well as bring forth the yeast genetics expertise of the core director to expedite troubleshooting or manipulation of screening systems to better meet their needs. The purpose of this core is to support the goals of the COBRE Project Leaders and other research scientist seeking to uncover novel protein-protein signaling complexes.
The Yeast 2-Hybrid Core will perform screens for protein-protein interactions using a variety of Yeast 2- Hybrid techniques, providing scientist with novel insights into mechanisms crucial for regulation of normal cellular homeostasis as well as those underlying pathogenic conditions.
|Guo, Qiusha; Wang, Yinqiu; Tripathi, Piyush et al. (2015) Adam10 mediates the choice between principal cells and intercalated cells in the kidney. J Am Soc Nephrol 26:149-59|
|Kim, Dae In; Birendra, K C; Zhu, Wenhong et al. (2014) Probing nuclear pore complex architecture with proximity-dependent biotinylation. Proc Natl Acad Sci U S A 111:E2453-61|
|Haugrud, Allison B; Zhuang, Yongxian; Coppock, Joseph D et al. (2014) Dichloroacetate enhances apoptotic cell death via oxidative damage and attenuates lactate production in metformin-treated breast cancer cells. Breast Cancer Res Treat 147:539-50|
|Finn, R; Evans, C C; Lee, L (2014) Strain-dependent brain defects in mouse models of primary ciliary dyskinesia with mutations in Pcdp1 and Spef2. Neuroscience 277:552-67|
|Floen, Miranda J; Forred, Benjamin J; Bloom, Elliot J et al. (2014) Thioredoxin-1 redox signaling regulates cell survival in response to hyperoxia. Free Radic Biol Med 75:167-77|
|Surendran, Kameswaran; Vitiello, Seasson P; Pearce, David A (2013) Lysosome dysfunction in the pathogenesis of kidney diseases. Pediatr Nephrol :|