Animal Care and Use Program Oklahoma Medical Research Foundation (OMRF) is one of the nation's oldest and most respected nonprofit biomedical research institutes and fosters a worldwide reputation for excellence by following an innovative cross-disciplinary approach to medical research. Dedicated to understanding and developing more effective treatments for human disease, OMRF's scientists routinely employ animal models and recognize the imperative of integrating the ethical and humane treatment of research animal subjects into the research endeavor. Since its incorporation in 1946, OMRF has grown substantially while consistently striving for excellence in its animal care and use program. In keeping with this, we intend to renovate our oldest vivarium, located in the Chapman building, to a state-of-the-art animal facility. OMRF houses more unique transgenic, mutant and immunocompromised mice than all of the major research institutions in Oklahoma combined. Many of these rodents serve as unique research models used by investigators not only locally but also nationally and worldwide. OMRF animal facilities provide housing and rederivation services for researchers from the OMRF, the University of Oklahoma, Oklahoma State University, Dean McGee Eye Institute and the Veterans Administration Hospital.

Public Health Relevance

of the Renovation to the Research Projects The Chapman vivarium is the vivarium closest to the research laboratories of Drs. Thompson, Alberola-lla, Kincade, Sun, Olson, and Wang (as well as additional non-COBRE investigators). Although some of the facility's structural design and architectural finish is outdated, the health status ofthe rodent colonies is presently at a high level similar to that in the rodent barrier at Bell;however, its entry requirements are less strict. As the barrier facility is preferably reserved for breeding colonies, the Chapman vivarium is ideal for experimental animals from the perspective of ease of accessibility, proximity to research labs, smaller rooms for separation of research colonies providing a quiet environment and contemporary housing equipment including individually ventilated cages and automatic watering systems. Two COBRE-supported Junior Investigators will use this facility. Lorin Olson, Ph.D. will use sophisticated mouse models to investigate the consequences of constitutive PDGF receptor signaling on the development of fibrosis. Weidong Wang, Ph.D. will evaluate the potential of various pancreatic progenitor cells to develop into functioning p-cells by transplanting them into mice and testing the ability of the mice to maintain glucose control. Many investigators at OMRF are sharing animals with other institutions, sending genetically engineered mice to and also receiving mutant lines from collaborators. There are about 50-60 incoming shipments per year with up to 6 in quarantine housing at the same time. In contrast to approved commercial vendors, nonapproved vendors/institutions often ship animals of partially unknown health status through a transport process that is less controlled from a biocontainment standpoint. Therefore, those rodents undergo a quarantine period and subsequent intense testing for infectious agents. In the high pace of the current research environment, keeping rodent colonies specific pathogen free is challenging, and isolated outbreaks of infections occur. These require subsequent treatment or depopulation ofthe respective housing area. If this has occurred at a collaborator's facility, it leads, in general, to diversion of the animals into the PHF facility rather than to the OMRF campus quarantine, even if those rodents had not been held directly in the affected rooms but simply in the same vivarium. The latter causes undesirable obstacles due to the remote location and introduction of unwanted variables into research experiments. To control this, animals must be rederived, delaying studies and wasting valuable resources. A quarantine area with separate, smaller rooms instead of a large quarantine room will allow isolation of shipments and acceptance of rodents with a less than perfect health report and therefore accelerate studies. Thus, the proposed renovations would benefit Junior Investigators supported directly by this COBRE as well as all the scientists at OMRF using mouse models.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
1P20GM103636-01A1
Application #
8466517
Study Section
Special Emphasis Panel (ZGM1-TWD-B (CB))
Project Start
Project End
Budget Start
2013-03-01
Budget End
2014-02-28
Support Year
1
Fiscal Year
2013
Total Cost
$300,000
Indirect Cost
Name
Oklahoma Medical Research Foundation
Department
Type
DUNS #
077333797
City
Oklahoma City
State
OK
Country
United States
Zip Code
73104
Lim, Hui-Ying; Wang, Weidong; Chen, Jianming et al. (2014) ROS regulate cardiac function via a distinct paracrine mechanism. Cell Rep 7:35-44
Towner, Rheal A; Wren, Jonathan D (2014) Prioritizing uncharacterized genes in the search for glioma biomarkers. CNS Oncol 3:93-5
Dozmorov, Mikhail G; Wren, Jonathan D; Alarcon-Riquelme, Marta E (2014) Epigenomic elements enriched in the promoters of autoimmunity susceptibility genes. Epigenetics 9:276-85
Bugreev, Dmitry V; Huang, Fei; Mazina, Olga M et al. (2014) HOP2-MND1 modulates RAD51 binding to nucleotides and DNA. Nat Commun 5:4198
Moktan, Hem; Guiraldelli, Michel F; Eyster, Craig A et al. (2014) Solution structure and DNA-binding properties of the winged helix domain of the meiotic recombination HOP2 protein. J Biol Chem 289:14682-91
Obeso, David; Pezza, Roberto J; Dawson, Dean (2014) Couples, pairs, and clusters: mechanisms and implications of centromere associations in meiosis. Chromosoma 123:43-55
Zhao, Weixing; Saro, Dorina; Hammel, Michal et al. (2014) Mechanistic insights into the role of Hop2-Mnd1 in meiotic homologous DNA pairing. Nucleic Acids Res 42:906-17
Tran, Kim; Li, Yu; Duan, Hongliang et al. (2014) Identification of small molecules that protect pancreatic ? cells against endoplasmic reticulum stress-induced cell death. ACS Chem Biol 9:2796-806
Pezza, Roberto J; Voloshin, Oleg N; Volodin, Alexander A et al. (2014) The dual role of HOP2 in mammalian meiotic homologous recombination. Nucleic Acids Res 42:2346-57
Lessard, Christopher J; Li, He; Adrianto, Indra et al. (2013) Variants at multiple loci implicated in both innate and adaptive immune responses are associated with Sjögren's syndrome. Nat Genet 45:1284-92

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