Animal Care and Use Program Oklahoma Medical Research Foundation (OMRF) is one of the nation's oldest and most respected nonprofit biomedical research institutes and fosters a worldwide reputation for excellence by following an innovative cross-disciplinary approach to medical research. Dedicated to understanding and developing more effective treatments for human disease, OMRF's scientists routinely employ animal models and recognize the imperative of integrating the ethical and humane treatment of research animal subjects into the research endeavor. Since its incorporation in 1946, OMRF has grown substantially while consistently striving for excellence in its animal care and use program. In keeping with this, we intend to renovate our oldest vivarium, located in the Chapman building, to a state-of-the-art animal facility. OMRF houses more unique transgenic, mutant and immunocompromised mice than all of the major research institutions in Oklahoma combined. Many of these rodents serve as unique research models used by investigators not only locally but also nationally and worldwide. OMRF animal facilities provide housing and rederivation services for researchers from the OMRF, the University of Oklahoma, Oklahoma State University, Dean McGee Eye Institute and the Veterans Administration Hospital.
of the Renovation to the Research Projects The Chapman vivarium is the vivarium closest to the research laboratories of Drs. Thompson, Alberola-lla, Kincade, Sun, Olson, and Wang (as well as additional non-COBRE investigators). Although some of the facility's structural design and architectural finish is outdated, the health status ofthe rodent colonies is presently at a high level similar to that in the rodent barrier at Bell;however, its entry requirements are less strict. As the barrier facility is preferably reserved for breeding colonies, the Chapman vivarium is ideal for experimental animals from the perspective of ease of accessibility, proximity to research labs, smaller rooms for separation of research colonies providing a quiet environment and contemporary housing equipment including individually ventilated cages and automatic watering systems. Two COBRE-supported Junior Investigators will use this facility. Lorin Olson, Ph.D. will use sophisticated mouse models to investigate the consequences of constitutive PDGF receptor signaling on the development of fibrosis. Weidong Wang, Ph.D. will evaluate the potential of various pancreatic progenitor cells to develop into functioning p-cells by transplanting them into mice and testing the ability of the mice to maintain glucose control. Many investigators at OMRF are sharing animals with other institutions, sending genetically engineered mice to and also receiving mutant lines from collaborators. There are about 50-60 incoming shipments per year with up to 6 in quarantine housing at the same time. In contrast to approved commercial vendors, nonapproved vendors/institutions often ship animals of partially unknown health status through a transport process that is less controlled from a biocontainment standpoint. Therefore, those rodents undergo a quarantine period and subsequent intense testing for infectious agents. In the high pace of the current research environment, keeping rodent colonies specific pathogen free is challenging, and isolated outbreaks of infections occur. These require subsequent treatment or depopulation ofthe respective housing area. If this has occurred at a collaborator's facility, it leads, in general, to diversion of the animals into the PHF facility rather than to the OMRF campus quarantine, even if those rodents had not been held directly in the affected rooms but simply in the same vivarium. The latter causes undesirable obstacles due to the remote location and introduction of unwanted variables into research experiments. To control this, animals must be rederived, delaying studies and wasting valuable resources. A quarantine area with separate, smaller rooms instead of a large quarantine room will allow isolation of shipments and acceptance of rodents with a less than perfect health report and therefore accelerate studies. Thus, the proposed renovations would benefit Junior Investigators supported directly by this COBRE as well as all the scientists at OMRF using mouse models.
|Duan, Hongliang; Arora, Daleep; Li, Yu et al. (2016) Identification of 1,2,3-triazole derivatives that protect pancreatic Î² cells against endoplasmic reticulum stress-mediated dysfunction and death through the inhibition of C/EBP-homologous protein expression. Bioorg Med Chem 24:2621-30|
|Borgogno, MarÃa V; Monti, Mariela R; Zhao, Weixing et al. (2016) Tolerance of DNA Mismatches in Dmc1 Recombinase-mediated DNA Strand Exchange. J Biol Chem 291:4928-38|
|Tsou, Pei-Suen; Wren, Jonathan D; Amin, M Asif et al. (2016) Histone Deacetylase 5 Is Overexpressed in Scleroderma Endothelial Cells and Impairs Angiogenesis via Repression of Proangiogenic Factors. Arthritis Rheumatol 68:2975-2985|
|Griffin, Timothy M; Humphries, Kenneth M; Kinter, Michael et al. (2016) Nutrient sensing and utilization: Getting to the heart of metabolic flexibility. Biochimie 124:74-83|
|Duan, Hongliang; Lee, Jae Wook; Moon, Sung Won et al. (2016) Discovery, Synthesis, and Evaluation of 2,4-Diaminoquinazolines as a Novel Class of Pancreatic Î²-Cell-Protective Agents against Endoplasmic Reticulum (ER) Stress. J Med Chem 59:7783-800|
|Siefert, Joseph C; Clowdus, Emily A; Sansam, Christopher L (2015) Cell cycle control in the early embryonic development of aquatic animal species. Comp Biochem Physiol C Toxicol Pharmacol 178:8-15|
|Larabee, Chelsea M; Georgescu, Constantin; Wren, Jonathan D et al. (2015) Expression profiling of the ubiquitin conjugating enzyme UbcM2 in murine brain reveals modest age-dependent decreases in specific neurons. BMC Neurosci 16:76|
|Dozmorov, Mikhail G; Adrianto, Indra; Giles, Cory B et al. (2015) Detrimental effects of duplicate reads and low complexity regions on RNA- and ChIP-seq data. BMC Bioinformatics 16 Suppl 13:S10|
|Pezza, Roberto J (2015) Mechanisms of chromosome segregation in meiosis--new views on the old problem of aneuploidy. FEBS J 282:2424-5|
|Lee, Chih-Ying; Horn, Henning F; Stewart, Colin L et al. (2015) Mechanism and regulation of rapid telomere prophase movements in mouse meiotic chromosomes. Cell Rep 11:551-63|
Showing the most recent 10 out of 33 publications