Over the past twenty years there has been a tremendous growth in the application of microfabrication technologies that are used to produce computer chips towards the development of microfluidic devices that can be used to study biological systems. Microchip based fluidic systems have been used to analyze the contents of single cells, measure the effect of channel size on ATP release from red blood cells as well as the study of the behavior and growth of C. elegans. The Microfabrication and Microfluidics Core (MMC) will provide the resources and personnel for the production of microfabricated devices that can be used by project investigators for their studies. It will also assist in the development of bioanalytical assays for small molecules and proteins. For the projects described in this COBRE proposal, the initial emphasis will be on the development of devices for trapping and imaging C. elegans, and manipulating zebrafish embryos. The devices developed in this core laboratory will make it possible for project investigators to explore the effect of genetic modifications of model organisms by fluorescence imaging in conjunction with the Genetics and Model Organism Core and the Synthetic Molecular Probes for in vivo Imaging core. In addition, microfluidics systems for maintaining tissue slices and cells will be developed and used for measurement of the release of neurotransmitters and other biomolecules. Lastly, basic fabrication methods will be available for the production of micron and submicron particles, channels and reservoirs for biophysical studies related to the molecular analysis of disease pathways.

Public Health Relevance

The Microfabrication and Microfluidics Core will develop lab-on-a-chip devices that can be used to facilitate investigations of the biochemistry pathways of diseases including neurological disorders, cardiovascular disease, cancer and pulmonary disorders.

Agency
National Institute of Health (NIH)
Type
Exploratory Grants (P20)
Project #
5P20GM103638-03
Application #
8691917
Study Section
Special Emphasis Panel (ZRR1)
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Kansas Lawrence
Department
Type
DUNS #
City
Lawrence
State
KS
Country
United States
Zip Code
66045
Zhao, Zheng; Yang, Yang; Zeng, Yong et al. (2016) A microfluidic ExoSearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis. Lab Chip 16:489-96
Grismer, Jesse L; Schulte 2nd, James A; Alexander, Alana et al. (2016) The Eurasian invasion: phylogenomic data reveal multiple Southeast Asian origins for Indian Dragon Lizards. BMC Evol Biol 16:43
Chakraborty, Aishik; Hui, Erica; Waring, Alan J et al. (2016) Combined effect of synthetic protein, Mini-B, and cholesterol on a model lung surfactant mixture at the air-water interface. Biochim Biophys Acta 1858:904-12
Huang, Wei; Beer, Rebecca L; Delaspre, Fabien et al. (2016) Sox9b is a mediator of retinoic acid signaling restricting endocrine progenitor differentiation. Dev Biol 418:28-39
Mosher, Laura J; Frau, Roberto; Pardu, Alessandra et al. (2016) Selective activation of D1 dopamine receptors impairs sensorimotor gating in Long-Evans rats. Br J Pharmacol 173:2122-34
Miller, Danny E; Smith, Clarissa B; Kazemi, Nazanin Yeganeh et al. (2016) Whole-Genome Analysis of Individual Meiotic Events in Drosophila melanogaster Reveals That Noncrossover Gene Conversions Are Insensitive to Interference and the Centromere Effect. Genetics 203:159-71
McGill, Jodi L; Nair, Arathy D S; Cheng, Chuanmin et al. (2016) Vaccination with an Attenuated Mutant of Ehrlichia chaffeensis Induces Pathogen-Specific CD4+ T Cell Immunity and Protection from Tick-Transmitted Wild-Type Challenge in the Canine Host. PLoS One 11:e0148229
Hasan, Anwarul; Waters, Renae; Roula, Boustany et al. (2016) Engineered Biomaterials to Enhance Stem Cell-Based Cardiac Tissue Engineering and Therapy. Macromol Biosci 16:958-77
McGill, Jodi L; Rusk, Rachel A; Guerra-Maupome, Mariana et al. (2016) Bovine Gamma Delta T Cells Contribute to Exacerbated IL-17 Production in Response to Co-Infection with Bovine RSV and Mannheimia haemolytica. PLoS One 11:e0151083
Park, Hyewon; Galbraith, Richard; Turner, Thaddeus et al. (2016) Loss of Ewing sarcoma EWS allele promotes tumorigenesis by inducing chromosomal instability in zebrafish. Sci Rep 6:32297

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