This application proposes to establish an Oklahoma Center of Biomedical Research Excellence (COBRE) in Structural Biology. Structural biology is a multidisciplinary research area that focuses on the important relationship between macromolecular structure and function. A structural biology approach can be taken to study any area of biological science and thus it has had a major impact on both basic and applied biomedical research. At the OU-Norman and OUHSC campuses, a number of research groups utilize a structural approach to study important biological macromolecules, in particular, proteins or nucleic acids that are promising targets for rationale drug design. Because of the multidisciplinary nature of structural biology, the training of students, postdocs, and researchers new to the field can be challenging. In addition, the instrumentation required for structural biology is both expensive and sophisticated. The overall objective of this proposal is to establish an Oklahoma COBRE in Structural Biology in order to build and nurture a critical mass of researchers in this area and support their research programs through shared resources and expertise.
The specific aims of this application are to: 1) Further the research activities and career development of junior investigators through senior mentorship and strengthening of research infrastructure;2) Create state-wide core facilities in macromolecular X-ray crystallography, high throughput crystallization, and protein expression/purification, which will provide users access to shared major instrumentation, staff support and research training;and 3) Promote structural biology in the State of Oklahoma through COBRE activities such as annual symposia, workshops, a seed grant program and core research facilities. Collectively, these specific aims are expected to increase the pace, competitiveness and success rate of structural biology research groups in Oklahoma as they seek major independent external grant support.

Public Health Relevance

Structural biology lies at the intersection of many different areas of biological sciences and thus has the potential of impacting numerous biomedically important fields. The specific research projects proposed herein have direct relevance to human diseases and conditions associated with aging, osteoporosis, diabetes, bacterial and parasitic infections.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
1P20GM103640-01
Application #
8216758
Study Section
Special Emphasis Panel (ZRR1-RI-B (01))
Program Officer
Liu, Yanping
Project Start
2012-08-01
Project End
2017-05-31
Budget Start
2012-08-01
Budget End
2013-05-31
Support Year
1
Fiscal Year
2012
Total Cost
$2,262,175
Indirect Cost
$400,138
Name
University of Oklahoma Norman
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
848348348
City
Norman
State
OK
Country
United States
Zip Code
73019
Mooers, Blaine H M (2016) Simplifying and enhancing the use of PyMOL with horizontal scripts. Protein Sci 25:1873-82
Cruz-Reyes, Jorge; Mooers, Blaine H M; Abu-Adas, Zakaria et al. (2016) DEAH-RHA helicase•Znf cofactor systems in kinetoplastid RNA editing and evolutionarily distant RNA processes. RNA Dis 3:
Isom, Catherine E; Menon, Smita K; Thomas, Leonard M et al. (2016) Crystal structure and DNA binding activity of a PadR family transcription regulator from hypervirulent Clostridium difficile R20291. BMC Microbiol 16:231
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Wang, Bing; Powell, Samantha M; Hessami, Neda et al. (2016) Crystal structures of two nitroreductases from hypervirulent Clostridium difficile and functionally related interactions with the antibiotic metronidazole. Nitric Oxide 60:32-39
Mooers, Blaine H M (2016) Direct-methods structure determination of a trypanosome RNA-editing substrate fragment with translational pseudosymmetry. Acta Crystallogr D Struct Biol 72:477-87
Wang, Bing; Thomas, Leonard M; Richter-Addo, George B (2016) Organometallic myoglobins: Formation of Fe-carbon bonds and distal pocket effects on aryl ligand conformations. J Inorg Biochem 164:1-4
Kumar, Vikas; Madina, Bhaskara R; Gulati, Shelly et al. (2016) REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes. J Biol Chem 291:5753-64
Madina, Bhaskara R; Kumar, Vikas; Mooers, Blaine H M et al. (2015) Native Variants of the MRB1 Complex Exhibit Specialized Functions in Kinetoplastid RNA Editing. PLoS One 10:e0123441

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