The development ofthe OU-Norman Macromolecular Crystallography Laboratory (MCL) as a COBRE Core facility will allow it to continue to provide expertise in both the crystallization of macromolecular samples and the collection of X-ray diffraction data for research groups at the University of Oklahoma and throughout the State of Oklahoma. This facility will provide the Junior Investigators (Jls), early career Investigators and other research groups involved in structural biology a state-of-the-art facility to advance their structural studies. The MCL can guide the research groups from the initial setup of crystallization plates to the solution and interpretation of the final three-dimensional structures. The MCL currently provides the capability of high throughput crystallization by the utilization of robotics Instrumentation. Utilization of the robotics for crystallization allows for efficient use of sample in determining macromolecular crystallization conditions. Crystals can be screened by MCL's current diffractometer setup but we are proposing an upgrade of our Xray generator to be able to provide the best possible X-ray crystallography facility for our Junior Investigators and other structural biology Investigators In the State. The increase In Intensity will benefit all investigators by providing the capability of screening and collection of X-ray diffraction data on smaller and generally weaker diffracting crystals. In addition to utilizing our current detector on the higher intensity generator we are also proposing to couple it to a CCD detector to provide a modern efficient diffraction system to allow the Jl's and other investigators statewide to conduct their research In structural biology.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM103640-02
Application #
8518422
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
2013-06-01
Budget End
2014-05-31
Support Year
2
Fiscal Year
2013
Total Cost
$496,397
Indirect Cost
Name
University of Oklahoma Norman
Department
Type
DUNS #
848348348
City
Norman
State
OK
Country
United States
Zip Code
73019
Mooers, Blaine H M (2016) Simplifying and enhancing the use of PyMOL with horizontal scripts. Protein Sci 25:1873-82
Cruz-Reyes, Jorge; Mooers, Blaine H M; Abu-Adas, Zakaria et al. (2016) DEAH-RHA helicase•Znf cofactor systems in kinetoplastid RNA editing and evolutionarily distant RNA processes. RNA Dis 3:
Isom, Catherine E; Menon, Smita K; Thomas, Leonard M et al. (2016) Crystal structure and DNA binding activity of a PadR family transcription regulator from hypervirulent Clostridium difficile R20291. BMC Microbiol 16:231
Lavey, Nathan P; Coker, Jesse A; Ruben, Eliza A et al. (2016) Sclerotiamide: The First Non-Peptide-Based Natural Product Activator of Bacterial Caseinolytic Protease P. J Nat Prod 79:1193-7
Vazquez Reyes, Carolina; Tangprasertchai, Narin S; Yogesha, S D et al. (2016) Nucleic Acid-Dependent Conformational Changes in CRISPR-Cas9 Revealed by Site-Directed Spin Labeling. Cell Biochem Biophys :
Wang, Bing; Powell, Samantha M; Hessami, Neda et al. (2016) Crystal structures of two nitroreductases from hypervirulent Clostridium difficile and functionally related interactions with the antibiotic metronidazole. Nitric Oxide 60:32-39
Mooers, Blaine H M (2016) Direct-methods structure determination of a trypanosome RNA-editing substrate fragment with translational pseudosymmetry. Acta Crystallogr D Struct Biol 72:477-87
Wang, Bing; Thomas, Leonard M; Richter-Addo, George B (2016) Organometallic myoglobins: Formation of Fe-carbon bonds and distal pocket effects on aryl ligand conformations. J Inorg Biochem 164:1-4
Kumar, Vikas; Madina, Bhaskara R; Gulati, Shelly et al. (2016) REH2C Helicase and GRBC Subcomplexes May Base Pair through mRNA and Small Guide RNA in Kinetoplastid Editosomes. J Biol Chem 291:5753-64
Madina, Bhaskara R; Kumar, Vikas; Mooers, Blaine H M et al. (2015) Native Variants of the MRB1 Complex Exhibit Specialized Functions in Kinetoplastid RNA Editing. PLoS One 10:e0123441

Showing the most recent 10 out of 31 publications