Inflammation is a complex biological process that involves many changes in the cell composition of circulating immune cells as well as tissues and organs. Identifying these changes in cell number and ratios, the status of various states of cell differentiation and apoptosis, and the expression patterns of cytokines and the signaling molecules that regulate the downstream pathways is essential in studying the process of inflammation. Many of these changes can be closely monitored by flow cytometry and sorting, and various other techniques based on micro-fluidic biotechnology such as multiplex ELISA assays for proteins and real time PCR for RNA. Many of these assays require expensive equipment and technical expertise that is not affordable by junior investigators such as the Target Faculty in this COBRE proposal on Dietary Supplements and Inflammation. In addition, setting operating parameters correctly and data interpretation when using these techniques is often difficult and requires experienced operators dedicated to the technology. The Flow Cytometry and Cell Sorting Core, which will be housed in the established Instrumentation Research Facility (IRF) at the University of South Carolina School of Medicine, will provide all of the technology and expertise required to complete these types of experiments proposed by the Target Investigators on this COBRE proposal. This core will be supervised by the current Director of the Flow Cytometry lab. There will be two faculty members who are affiliated with the IRF available for assistance with experimental design, operation of equipment, and training on micro-fluidics instrumentation. In addition to individual interactions, didactic courses, seminars and workshops are often sponsored through the IRF. These will be made available to members of the Target Investigator laboratories.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
7P20GM103641-02
Application #
8531304
Study Section
Special Emphasis Panel (ZRR1-RI-4)
Project Start
Project End
Budget Start
2013-05-31
Budget End
2014-05-30
Support Year
2
Fiscal Year
2013
Total Cost
$132,205
Indirect Cost
$35,705
Name
University of South Carolina at Columbia
Department
Type
DUNS #
041387846
City
Columbia
State
SC
Country
United States
Zip Code
29208
Zhou, Juhua; Chaudhry, Hina; Zhong, Yin et al. (2015) Dysregulation in microRNA expression in peripheral blood mononuclear cells of sepsis patients is associated with immunopathology. Cytokine 71:89-100
Sido, Jessica Margaret; Nagarkatti, Prakash S; Nagarkatti, Mitzi (2015) Role of Endocannabinoid Activation of Peripheral CB1 Receptors in the Regulation of Autoimmune Disease. Int Rev Immunol 34:403-14
Enos, Reilly T; Velázquez, Kandy T; Murphy, E Angela (2014) Insight into the impact of dietary saturated fat on tissue-specific cellular processes underlying obesity-related diseases. J Nutr Biochem 25:600-12
Zhou, Juhua; Nagarkatti, Prakash; Zhong, Yin et al. (2014) Dysregulation in microRNA expression is associated with alterations in immune functions in combat veterans with post-traumatic stress disorder. PLoS One 9:e94075
Enos, Reilly T; Velázquez, Kandy T; McClellan, Jamie L et al. (2014) Reducing the dietary omega-6:omega-3 utilizing ?-linolenic acid; not a sufficient therapy for attenuating high-fat-diet-induced obesity development nor related detrimental metabolic and adipose tissue inflammatory outcomes. PLoS One 9:e94897
Cuenca-García, Magdalena; Artero, Enrique G; Sui, Xuemei et al. (2014) Dietary indices, cardiovascular risk factors and mortality in middle-aged adults: findings from the Aerobics Center Longitudinal Study. Ann Epidemiol 24:297-303.e2
Yang, Xiaoming; Hegde, Venkatesh L; Rao, Roshni et al. (2014) Histone modifications are associated with ?9-tetrahydrocannabinol-mediated alterations in antigen-specific T cell responses. J Biol Chem 289:18707-18
Rutledge, Katy; Cheng, Qingsu; Pryzhkova, Marina et al. (2014) Enhanced differentiation of human embryonic stem cells on extracellular matrix-containing osteomimetic scaffolds for bone tissue engineering. Tissue Eng Part C Methods 20:865-74
Liu, Qing; Wang, Junfeng; Liang, Qiaoli et al. (2014) Sparstolonin B attenuates hypoxia-reoxygenation-induced cardiomyocyte inflammation. Exp Biol Med (Maywood) 239:376-84
Chakrabarti, Mrinmay; Haque, Azizul; Banik, Naren L et al. (2014) Estrogen receptor agonists for attenuation of neuroinflammation and neurodegeneration. Brain Res Bull 109:22-31

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