The COBRE Neuroimaging and E lectrophysiology Facility (NIEF) will provide state-of-the-art instrumentation and support to Center for Neuroplasticity investigators and the Iarger UPR biomedical research community. For the past 20 years, the University of Puerto Rico, has strengthened research quality in an effort to facilitate a transition towards competitive scientific research projects. We propose to expand these efforts by establishing a facility equipped with modern imaging and el ectrophysiology instrumentation, This desire is driven by the increasing importance that imaging and electrophysiology data has gained in the field neuroplasticity. The first step (Specific Aim 1) will be to update and upgrade the Institute of Neurobiology Neuroimaging Facility to create the NIEF. The NIEF will provide users the capability to study neuroplasticity by taking advantage of the state of the art instrumentation. The second step (Specific Aim 2) will be to integrate imaging and electrophysiological applications. Of key importance will be to provide the N lEF the sensitivity to detect fluorescence emissions from diverse biological preparations while performing confocal microscopy and/or two photon microscopy and to enable electrophysiological measurements to be per formed simultaneously. Recent research provides an example of the power of coupling patch clamp electrophysiology techniques with ratiometric calcium imaging. In addition, the equipment to be purchased will allow users to perform fluorescence emission studies and realtime live imaging experiments. The third step (Specific Aim 3) in the creation of the NIEF will be to increase the equipment flexibility and detection sensitivity for fluorescence-based applications. Of key importance to this aim will be the introduction of complex multilabeling experiments using emission fingerprinting and automatic tuning of two photon lasers. Fulfillment of these objectives enable the COBRE NIEF to provide training opportunities for students, postdoctoral fellows and faculty in imaging techniques for the study of biological preparations. The NIEF will be a completely self-sustained neuroimaging research facility and a role model for Puerto Rico and the Caribbean.

Public Health Relevance

This Neuroimaging and Electrophysiology Facility will play an integral part in advancing the research objectives of the COBRE Center for Neuroplasticity. It will thereby facilitate the COBRE to create an innovative state-of-the-art neuroscience research center at the University of Puerto Rico.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM103642-02
Application #
8687679
Study Section
Special Emphasis Panel (ZGM1-TWD-B)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
2
Fiscal Year
2014
Total Cost
$325,594
Indirect Cost
$97,777
Name
University of Puerto Rico Med Sciences
Department
Type
DUNS #
948108063
City
San Juan
State
PR
Country
United States
Zip Code
00936
Delgado-Peraza, Francheska; Ahn, Kwang H; Nogueras-Ortiz, Carlos et al. (2016) Mechanisms of Biased β-Arrestin-Mediated Signaling Downstream from the Cannabinoid 1 Receptor. Mol Pharmacol 89:618-29
Padilla-Morales, Luis F; Colón-Sáez, José O; González-Nieves, Joel E et al. (2016) Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology. Data Brief 6:433-7
Duprey-Díaz, Mildred V; Blagburn, Jonathan M; Blanco, Rosa E (2016) Exogenous Modulation of Retinoic Acid Signaling Affects Adult RGC Survival in the Frog Visual System after Optic Nerve Injury. PLoS One 11:e0162626
Melendez, Roberto I; Roman, Cristina; Capo-Velez, Coral M et al. (2016) Decreased glial and synaptic glutamate uptake in the striatum of HIV-1 gp120 transgenic mice. J Neurovirol 22:358-65
Colón, Jennifer M; Torrado, Aranza I; Cajigas, Ámbar et al. (2016) Tamoxifen Administration Immediately or 24 Hours after Spinal Cord Injury Improves Locomotor Recovery and Reduces Secondary Damage in Female Rats. J Neurotrauma 33:1696-708
Ramos, Félix M; Delgado-Vélez, Manuel; Ortiz, Ángel L et al. (2016) Expression of CHRFAM7A and CHRNA7 in neuronal cells and postmortem brain of HIV-infected patients: considerations for HIV-associated neurocognitive disorder. J Neurovirol 22:327-35
Nogueras-Ortiz, Carlos; Yudowski, Guillermo A (2016) The Multiple Waves of Cannabinoid 1 Receptor Signaling. Mol Pharmacol 90:620-626
Morales-Cruz, Moraima; Cruz-Montañez, Alejandra; Figueroa, Cindy M et al. (2016) Combining Stimulus-Triggered Release and Active Targeting Strategies Improves Cytotoxicity of Cytochrome c Nanoparticles in Tumor Cells. Mol Pharm 13:2844-54
Báez-Pagán, Carlos A; Del Hoyo-Rivera, Natalie; Quesada, Orestes et al. (2016) Heterogeneous Inhibition in Macroscopic Current Responses of Four Nicotinic Acetylcholine Receptor Subtypes by Cholesterol Enrichment. J Membr Biol 249:539-49
Martinez, Namyr A; Ayala, Alondra M; Martinez, Magdiel et al. (2016) Caveolin-1 Regulates the P2Y2 Receptor Signaling in Human 1321N1 Astrocytoma Cells. J Biol Chem 291:12208-22

Showing the most recent 10 out of 58 publications