The broad and long term goal of the Animal Models Core is provide consistent care, experimental procedures, and husbandry are important to the success ofthe projects. Each promising investigator has limited animal experimental experience, but needs to verify in vitro findings in vivo. Therefore, the Animal Model Core (AMC) will ensure project experimental consistency, compliance with federal animal welfare regulations, and effective training of project leaders and personnel in animal experimentation. Dr. Hinsdale will serve as the AMC director, and along with technical staff will provide basic services (e.g. Colony management and husbandry, biosafety containment and housing of animals, infection of animals and harvesting of samples for analyses, and pulmonary function tests). The current experimental animal models will be mice. The four projects that are proposed require mice to further verify in vivo findings arising from the proposed in vitro experiments. In project 1, using some remarkable viral strains, BALB/c mice will be used to determine the role of the human Respiratory Syncytial Virus (RSV) Matrix protein in viral infection. In project 2, influenza infected mice will be used to validate important host factors in an innovative in vitro respiratory model. In project 3, using a novel mouse model of acute lung injury, challenged mice will be used to mechanistically explore the exciting and potential therapeutic role of a unique protein on acute bacterial lung injury. In project 4, mice will be used to determine mechanistically the pathogenic role that specific chemokine and cytokine mediators have in acute lung injury due to influenza and to determine the pioneering therapeutic combinations for ameliorating this acute lung injury. In all, the up-and-coming young investigators of this application will be breaking new ground in pulmonary disease and gaining important animal model training to prepare them for promising independent research careers.
The AMC as part of the proposed COBRE Center will provide a critical resource for the proposed studies into respiratory viral and bacterial diseases and to the discovery of mechanisms of lung injury and into therapeutics for infections of the lung. Furthermore, the AMC will be very crucial to the training of young investigators for independent research utilizing animal models of disease.
|Ektate, Kalyani; Munteanu, Maria Cristina; Ashar, Harshini et al. (2018) Chemo-immunotherapy of colon cancer with focused ultrasound and Salmonella-laden temperature sensitive liposomes (thermobots). Sci Rep 8:13062|
|Haghnegahdar, Ahmadreza; Feng, Yu; Chen, Xiaole et al. (2018) Computational Analysis of Deposition and Translocation of Inhaled Nicotine and Acrolein in the Human Body with E-cigarette Puffing Topographies. Aerosol Sci Technol 52:483-493|
|Zhu, Liqian; Jones, Clinton (2018) The canonical Wnt/?-catenin signaling pathway stimulates herpes simplex virus 1 productive infection. Virus Res 256:29-37|
|Ashar, Harshini K; Mueller, Nathan C; Rudd, Jennifer M et al. (2018) The Role of Extracellular Histones in Influenza Virus Pathogenesis. Am J Pathol 188:135-148|
|Patil, Girish; Zhao, Mengmeng; Song, Kun et al. (2018) TRIM41-Mediated Ubiquitination of Nucleoprotein Limits Influenza A Virus Infection. J Virol 92:|
|Senavirathna, Lakmini Kumari; Huang, Chaoqun; Yang, Xiaoyun et al. (2018) Hypoxia induces pulmonary fibroblast proliferation through NFAT signaling. Sci Rep 8:2709|
|Booth, J Leland; Duggan, Elizabeth S; Patel, Vineet I et al. (2018) Gene expression profiling of primary human type I alveolar epithelial cells exposed to Bacillus anthracis spores reveals induction of neutrophil and monocyte chemokines. Microb Pathog 121:9-21|
|Maria, Zahra; Lacombe, Véronique A (2018) Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay. Methods Mol Biol 1713:229-240|
|Workman, Aspen; Zhu, Liqian; Keel, Brittney N et al. (2018) The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency. J Virol 92:|
|McGill, Jodi L; Wang, Ying; Ganta, Chanran K et al. (2018) Antigen-Specific CD4+CD8+ Double-Positive T Cells Are Increased in the Blood and Spleen During Ehrlichia chaffeensis Infection in the Canine Host. Front Immunol 9:1585|
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