The specific aim of the Immunopathology Core Program (IPC) is to provide pathology and immunology support to COBRE and other Oklahoma Centerfor Respiratory and Infectious Diseases (OCRID) investigators. Services provided by the IPC include a broad range of pathology support including gross necropsy and tissue collection, tissue processing, sectioning of either frozen or paraffin-embedded tissues, routine and special stains, immunohistochemistry (IHC), immunofluorescence (IFC), and coordination of ancillary services (clinical pathology, bacteriology, virology, toxicology). Immune responses important in the development of disease are characterized by evaluation of cell-mediated immunity (ELISPOT, flow cytometry for intracellular IFNy) and phenotypic identification of inflammatory cells within lesions (IFC, IHC, flow cytometry). The IPC will also coordinate with the Animal Models Core (AMC) and Molecular Biology Core (MBC) to assist investigators with humoral immunity (serology, ELISA) and quantitation of cytokine gene expression (real-time PCR) respectively. To achieve this aim, the IPC is staffed by three board-certified pathologists with experience in animal models and respiratory/infectious disease research and will recruit an experienced, extensively cross-trained technician. The IPC has the resources in place to serve as the foundation and a strong institutional commitment to ensure development of the IPC into a self-sustaining facility integral to the research infrastructure of OCRID.

Public Health Relevance

By its very nature, comparative medicine research includes the full spectrum of basic and clinical sciences using animal model systems. A CRITICAL component of the infrastructure for animal-based research is immunopathology support provided by an organized, centralized core-service.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM103648-02
Application #
8686890
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
2
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Oklahoma State University Stillwater
Department
Type
DUNS #
City
Stillwater
State
OK
Country
United States
Zip Code
74078
Mishra, Amarjit; Guo, Yujie; Zhang, Li et al. (2016) A Critical Role for P2X7 Receptor-Induced VCAM-1 Shedding and Neutrophil Infiltration during Acute Lung Injury. J Immunol 197:2828-37
Wu, Wenxin; Zhang, Wei; Booth, J Leland et al. (2016) Human primary airway epithelial cells isolated from active smokers have epigenetically impaired antiviral responses. Respir Res 17:111
Wang, Lingyan; Li, Wenjun; Li, Shitao (2016) A High Throughput Assay for Screening Host Restriction Factors and Antivirals Targeting Influenza A Virus. Front Microbiol 7:858
Wu, Lei; Guo, Xin; Wang, Weiqun et al. (2016) Molecular aspects of β, β-carotene-9', 10'-oxygenase 2 in carotenoid metabolism and diseases. Exp Biol Med (Maywood) 241:1879-1887
Sahoo, Kaustuv; Koralege, Rangika S Hikkaduwa; Flynn, Nicholas et al. (2016) Nanoparticle Attachment to Erythrocyte Via the Glycophorin A Targeted ERY1 Ligand Enhances Binding without Impacting Cellular Function. Pharm Res 33:1191-203
Patel, Vineet Indrajit; Metcalf, Jordan Patrick (2016) Identification and characterization of human dendritic cell subsets in the steady state: a review of our current knowledge. J Investig Med 64:833-47
Rudd, Jennifer M; Ashar, Harshini K; Chow, Vincent Tk et al. (2016) Lethal Synergism between Influenza and Streptococcus pneumoniae. J Infect Pulm Dis 2:
Narasaraju, Teluguakula; Harshini, Ashar (2016) Neutrophils as Possible Therapeutic Targets in Severe Influenza Pneumonia. J Infect Pulm Dis 2:
Xiao, Xiao; Huang, Chaoqun; Zhao, Chunling et al. (2015) Regulation of myofibroblast differentiation by miR-424 during epithelial-to-mesenchymal transition. Arch Biochem Biophys 566:49-57
Wu, Wenxin; Zhang, Wei; Duggan, Elizabeth S et al. (2015) RIG-I and TLR3 are both required for maximum interferon induction by influenza virus in human lung alveolar epithelial cells. Virology 482:181-8

Showing the most recent 10 out of 20 publications