The COBRE projects utilize molecular biology approaches to understand the molecular mechanisms of respiratory diseases caused by respiratory pathogens including Respiratory Syncytial Virus, influenza virus, and bacteria. The objective of the Molecular Biology Core is to provide centralized services and technical expertise in molecular biology for promising junior investigators and other Center investigators.
The specific aims of the core are as follows: 1) Design and construct RNAi vectors for gene silencing, 2) Design and construct overexpression vectors for transgene expression, 3) Provide support for mutagenesis, 3) Provide support for gene and pathway reporter assays, 4) Provide support for efficient transfection using adenovirus and lentivirus vectors or the Amaxa system, 5) Provide support for real-time PCR gene qualification, 6) Provide support for DNA microarray analyses, and 7) Provide advice and consultation on various aspects of molecular biology to COBRE and Center investigators and develop or adopt new methods as needed.
|Zhu, Liqian; Thunuguntla, Prasanth; Liu, Yilin et al. (2017) The ?-catenin signaling pathway stimulates bovine herpesvirus 1 productive infection. Virology 500:91-95|
|Feng, Yu; Zhao, Jianan; Chen, Xiaole et al. (2017) An In Silico Subject-Variability Study of Upper Airway Morphological Influence on the Airflow Regime in a Tracheobronchial Tree. Bioengineering (Basel) 4:|
|Eberle, R; Maxwell, L K; Nicholson, S et al. (2017) Genome sequence variation among isolates of monkey B virus (Macacine alphaherpesvirus 1) from captive macaques. Virology 508:26-35|
|Wang, Xiaoqiu; Wu, Wenxin; Zhang, Wei et al. (2017) RIG-I overexpression decreases mortality of cigarette smoke exposed mice during influenza A virus infection. Respir Res 18:166|
|Premaratne, Gayan; Al Mubarak, Zainab H; Senavirathna, Lakmini et al. (2017) Measuring Ultra-low Levels of Nucleotide Biomarkers Using Quartz Crystal Microbalance and SPR Microarray Imaging Methods: A Comparative Analysis. Sens Actuators B Chem 253:368-375|
|Fu, Bishi; Zhao, Mengmeng; Wang, Lingyan et al. (2017) RNAi Screen and Proteomics Reveal NXF1 as a Novel Regulator of IRF5 Signaling. Sci Rep 7:2683|
|Wu, Lei; Guo, Xin; Hartson, Steven D et al. (2017) Lack of ?, ?-carotene-9', 10'-oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice. Mol Nutr Food Res 61:|
|Fu, Bishi; Wang, Lingyan; Li, Shitao et al. (2017) ZMPSTE24 defends against influenza and other pathogenic viruses. J Exp Med 214:919-929|
|Zeng, Xiangming; Huang, Chaoqun; Senavirathna, Lakmini et al. (2017) miR-27b inhibits fibroblast activation via targeting TGF? signaling pathway. BMC Cell Biol 18:9|
|Zhu, Liqian; Thompson, Jesse; Ma, Fangrui et al. (2017) Effects of the synthetic corticosteroid dexamethasone on bovine herpesvirus 1 productive infection. Virology 505:71-79|
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