The COBRE projects utilize molecular biology approaches to understand the molecular mechanisms of respiratory diseases caused by respiratory pathogens including Respiratory Syncytial Virus, influenza virus, and bacteria. The objective of the Molecular Biology Core is to provide centralized services and technical expertise in molecular biology for promising junior investigators and other Center investigators.
The specific aims of the core are as follows: 1) Design and construct RNAi vectors for gene silencing, 2) Design and construct overexpression vectors for transgene expression, 3) Provide support for mutagenesis, 3) Provide support for gene and pathway reporter assays, 4) Provide support for efficient transfection using adenovirus and lentivirus vectors or the Amaxa system, 5) Provide support for real-time PCR gene qualification, 6) Provide support for DNA microarray analyses, and 7) Provide advice and consultation on various aspects of molecular biology to COBRE and Center investigators and develop or adopt new methods as needed.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM103648-02
Application #
8686891
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
2
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Oklahoma State University Stillwater
Department
Type
DUNS #
City
Stillwater
State
OK
Country
United States
Zip Code
74078
Mishra, Amarjit; Guo, Yujie; Zhang, Li et al. (2016) A Critical Role for P2X7 Receptor-Induced VCAM-1 Shedding and Neutrophil Infiltration during Acute Lung Injury. J Immunol 197:2828-37
Wu, Wenxin; Zhang, Wei; Booth, J Leland et al. (2016) Human primary airway epithelial cells isolated from active smokers have epigenetically impaired antiviral responses. Respir Res 17:111
Wang, Lingyan; Li, Wenjun; Li, Shitao (2016) A High Throughput Assay for Screening Host Restriction Factors and Antivirals Targeting Influenza A Virus. Front Microbiol 7:858
Wu, Lei; Guo, Xin; Wang, Weiqun et al. (2016) Molecular aspects of β, β-carotene-9', 10'-oxygenase 2 in carotenoid metabolism and diseases. Exp Biol Med (Maywood) 241:1879-1887
Sahoo, Kaustuv; Koralege, Rangika S Hikkaduwa; Flynn, Nicholas et al. (2016) Nanoparticle Attachment to Erythrocyte Via the Glycophorin A Targeted ERY1 Ligand Enhances Binding without Impacting Cellular Function. Pharm Res 33:1191-203
Patel, Vineet Indrajit; Metcalf, Jordan Patrick (2016) Identification and characterization of human dendritic cell subsets in the steady state: a review of our current knowledge. J Investig Med 64:833-47
Rudd, Jennifer M; Ashar, Harshini K; Chow, Vincent Tk et al. (2016) Lethal Synergism between Influenza and Streptococcus pneumoniae. J Infect Pulm Dis 2:
Narasaraju, Teluguakula; Harshini, Ashar (2016) Neutrophils as Possible Therapeutic Targets in Severe Influenza Pneumonia. J Infect Pulm Dis 2:
Xiao, Xiao; Huang, Chaoqun; Zhao, Chunling et al. (2015) Regulation of myofibroblast differentiation by miR-424 during epithelial-to-mesenchymal transition. Arch Biochem Biophys 566:49-57
Wu, Wenxin; Zhang, Wei; Duggan, Elizabeth S et al. (2015) RIG-I and TLR3 are both required for maximum interferon induction by influenza virus in human lung alveolar epithelial cells. Virology 482:181-8

Showing the most recent 10 out of 20 publications