Abstract

All the Phase II CoBRE projects utilize molecular biology approaches to understand the molecular mechanisms of respiratory diseases caused by respiratory pathogens, including influenza virus (Project 1 and Project 2), Streptococcus pneumoniae (Project 3) and Pseudomonas aeruginosa (Project 4). The overall objective of the Molecular Biology Core (MBC) is to provide centralized services and technical expertise in molecular biology for Phase II CoBRE project investigators, pilot project investigators, and other investigators.
The specific aims of the MBC are to provide services and support for 1) functional studies using RNAi and CRISPR/Cas9, transgene overexpression, and mutagenesis/deletion, 2) genome-wide gene profiling using RNA sequencing, 3) molecular assays including reporter assays and real-time quantitative PCR, 4) reagents for respiratory and infectious disease research including primary lung cells, respiratory viruses and bacteria, and 5) advice, consultation and training on various aspects of molecular biology and developing or adopting new methods as needed.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM103648-07
Application #
9745621
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2019-07-01
Budget End
2020-06-30
Support Year
7
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
Oklahoma State University Stillwater
Department
Type
DUNS #
049987720
City
Stillwater
State
OK
Country
United States
Zip Code
74078

  Publications

Ektate, Kalyani; Munteanu, Maria Cristina; Ashar, Harshini et al. (2018) Chemo-immunotherapy of colon cancer with focused ultrasound and Salmonella-laden temperature sensitive liposomes (thermobots). Sci Rep 8:13062
Haghnegahdar, Ahmadreza; Feng, Yu; Chen, Xiaole et al. (2018) Computational Analysis of Deposition and Translocation of Inhaled Nicotine and Acrolein in the Human Body with E-cigarette Puffing Topographies. Aerosol Sci Technol 52:483-493
Zhu, Liqian; Jones, Clinton (2018) The canonical Wnt/?-catenin signaling pathway stimulates herpes simplex virus 1 productive infection. Virus Res 256:29-37
Ashar, Harshini K; Mueller, Nathan C; Rudd, Jennifer M et al. (2018) The Role of Extracellular Histones in Influenza Virus Pathogenesis. Am J Pathol 188:135-148
Patil, Girish; Zhao, Mengmeng; Song, Kun et al. (2018) TRIM41-Mediated Ubiquitination of Nucleoprotein Limits Influenza A Virus Infection. J Virol 92:
Senavirathna, Lakmini Kumari; Huang, Chaoqun; Yang, Xiaoyun et al. (2018) Hypoxia induces pulmonary fibroblast proliferation through NFAT signaling. Sci Rep 8:2709
Booth, J Leland; Duggan, Elizabeth S; Patel, Vineet I et al. (2018) Gene expression profiling of primary human type I alveolar epithelial cells exposed to Bacillus anthracis spores reveals induction of neutrophil and monocyte chemokines. Microb Pathog 121:9-21
Maria, Zahra; Lacombe, Véronique A (2018) Quantification of Cell-Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeling Assay. Methods Mol Biol 1713:229-240
Workman, Aspen; Zhu, Liqian; Keel, Brittney N et al. (2018) The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency. J Virol 92:
McGill, Jodi L; Wang, Ying; Ganta, Chanran K et al. (2018) Antigen-Specific CD4+CD8+ Double-Positive T Cells Are Increased in the Blood and Spleen During Ehrlichia chaffeensis Infection in the Canine Host. Front Immunol 9:1585

Showing the most recent 10 out of 55 publications