The Cell Isolation/Organ Function Core provides a unique skill set and expertise to Rl vascular biologists by providing quality assurance in isolation, characterization, and propagation of vascular derived cells and fibroblasts and cardiopulmonary organ function analyses. The centralization of the cell isolation and organ function measurements will help investigators minimize the variability in sample preparation thus providing uniformity in data acquisition throughout all COBRE Projects and for other Rl vascular biologists. Isolation, characterization, and propagation of the cells is time-consuming and costly;thus the services provided by this Core permit the Project and Pilot Investigators to focus their efforts on aspects of their research endeavors related to experimental design, execution and interpretation. Additionally, the Core provides the expertise for assessing cardiac function, pressure-volume system for simultaneous high-fidelity intracardiac pressure-volume analysis, as well as lung function. As our Project and Pilot Investigators and/or other Rl vascular biologists experiments develop, so will their cell isolation needs;thus the Core will expand to meet these needs by providing expertise in the isolation of primary cultures of cardiac or pulmonary endothelial cells, fibroblasts, as well as vascular smooth muscle cells. Most of the needed equipment, facilities, and personnel are already in place at the Vascuiar Research Laboratory at the Providence VAIVIC, thus the Core will continue to serve as a resource for investigators in Rl after the COBRE funding is complete. The overall goal of the Core is to facilitate the scientific objectives of the Project and Pilot Investigators by providing essential services in: i) Isolation of pulmonary and cardiac endothelial cell and ventricular fibroblast cells;characterization, propagation, and biochemical analysis;ii) Endothelial progenitor cell and microparticle isolation from patient blood;iii) Measurement of heart and lung function. Secondary goals of this Core are to enhance reproducibility of data and experimental endpoints, and increasing the efficiency and productivity of each project.

Public Health Relevance

The Cell Isolation/Organ Function Core will isolate cells from the heart and lungs of rodents and perform analyses to test the function of the hearts or lungs in rodent for scientist in Rl, working at academic institutions or affiliated institutions such as the Providence VAMC, Rhode Island Hospital, Bryant University, Brown University, Rhode Island College, University of Rhode Island, and other Brown- affiliated hospitals.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
1P20GM103652-01A1
Application #
8465676
Study Section
Special Emphasis Panel (ZGM1-TWD-B (CB))
Project Start
Project End
Budget Start
2013-09-20
Budget End
2014-05-31
Support Year
1
Fiscal Year
2013
Total Cost
$289,679
Indirect Cost
Name
Ocean State Research Institute, Inc.
Department
Type
DUNS #
848476271
City
Providence
State
RI
Country
United States
Zip Code
02908
Aliotta, Jason M; Pereira, Mandy; Wen, Sicheng et al. (2016) Exosomes induce and reverse monocrotaline-induced pulmonary hypertension in mice. Cardiovasc Res 110:319-30
(2016) Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12:1-222
Heydari, Bobak; Abdullah, Shuaib; Pottala, James V et al. (2016) Effect of Omega-3 Acid Ethyl Esters on Left Ventricular Remodeling After Acute Myocardial Infarction: The OMEGA-REMODEL Randomized Clinical Trial. Circulation 134:378-91
Monaghan, Sean F; Chung, Chun-Shiang; Chen, Yaping et al. (2016) Soluble programmed cell death receptor-1 (sPD-1): a potential biomarker with anti-inflammatory properties in human and experimental acute respiratory distress syndrome (ARDS). J Transl Med 14:312
Sakhatskyy, Pavlo; Wang, Zhengke; Borgas, Diana et al. (2016) Double-Hit Mouse Model of Cigarette Smoke Priming for Acute Lung Injury. Am J Physiol Lung Cell Mol Physiol :ajplung.00436.2016
Addison, Daniel; Farhad, Hoshang; Shah, Ravi V et al. (2016) Effect of Late Gadolinium Enhancement on the Recovery of Left Ventricular Systolic Function After Pulmonary Vein Isolation. J Am Heart Assoc 5:
Lomas-Neira, Joanne L; Heffernan, Daithi S; Ayala, Alfred et al. (2016) BLOCKADE OF ENDOTHELIAL GROWTH FACTOR, ANGIOPOIETIN-2, REDUCES INDICES OF ARDS AND MORTALITY IN MICE RESULTING FROM THE DUAL-INSULTS OF HEMORRHAGIC SHOCK AND SEPSIS. Shock 45:157-65
Feng, Jun; Liu, Yuhong; Sabe, Ashraf A et al. (2016) Differential impairment of adherens-junction expression/phosphorylation after cardioplegia in diabetic versus non-diabetic patients. Eur J Cardiothorac Surg 49:937-43
Korre, Maria; Porto, Luiz Guilherme G; Farioli, Andrea et al. (2016) Effect of Body Mass Index on Left Ventricular Mass in Career Male Firefighters. Am J Cardiol 118:1769-1773
Yoon, Pyoung Oh; Park, Jin Wook; Lee, Chang-Min et al. (2016) Self-assembled Micelle Interfering RNA for Effective and Safe Targeting of Dysregulated Genes in Pulmonary Fibrosis. J Biol Chem 291:6433-46

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