The COBRE Core C: Cell Analysis and Sorting is located at the University of Rhode Island, Providence. The role of Core C will be to provide expertise for the design, trouble shooting and analysis of assays for the COBRE investigators. This expertise will be provided by the CMI core director, Loren Fast, Ph.D., who has over 30 years of experience as a cellular immunologist studying immune responses of both human and murine lymphocytes. The CMI core manager, Joe Derosiers, B.S., has over 3 years of experience maintaining and running the core instrumentation.
The second aim i s provide and maintain instrumentation that can be used to measure the responses of immune assays. The instrumentation available for use by the COBRE investigators includes an BD LSRll flow cytometer with four lasers capable of reading at least 12 colors, an AUTOMacs Pro for cell separation, ELISPOT and ELISA readers for obtaining results from these assays, an AKTA Avant FPLC and a controlled rate freezer for storage of cells. We are also proposing to acquire a Miltenyi MACSQuant Analyzer which will be good for routine analysis of sample containing up to 7 colors. This will free up the LSRll for analysis of more complicated samples. The cell-mediated immunity (CMI) core for the Ul 9 TRIAD grant is already in place at University of Rhode Island and the efforts of Core C with synergize with the efforts of the CMI core.

Public Health Relevance

The COBRE Core C: Cell Analysis and Sorting will serve as a resource for the other COBRE investigators by providing expert advice as they develop and carry out their experiments and provide special instruments for their use in measuring the results of their experiments.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM104317-02
Application #
8727076
Study Section
Special Emphasis Panel (ZGM1-TWD-A)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
2
Fiscal Year
2014
Total Cost
$69,762
Indirect Cost
$20,151
Name
University of Rhode Island
Department
Type
DUNS #
144017188
City
Kingston
State
RI
Country
United States
Zip Code
02881
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