The COBRE Core C: Cell Analysis and Sorting is located at the University of Rhode Island, Providence. The role of Core C will be to provide expertise for the design, trouble shooting and analysis of assays for the COBRE investigators. This expertise will be provided by the CMI core director, Loren Fast, Ph.D., who has over 30 years of experience as a cellular immunologist studying immune responses of both human and murine lymphocytes. The CMI core manager, Joe Derosiers, B.S., has over 3 years of experience maintaining and running the core instrumentation.
The second aim i s provide and maintain instrumentation that can be used to measure the responses of immune assays. The instrumentation available for use by the COBRE investigators includes an BD LSRll flow cytometer with four lasers capable of reading at least 12 colors, an AUTOMacs Pro for cell separation, ELISPOT and ELISA readers for obtaining results from these assays, an AKTA Avant FPLC and a controlled rate freezer for storage of cells. We are also proposing to acquire a Miltenyi MACSQuant Analyzer which will be good for routine analysis of sample containing up to 7 colors. This will free up the LSRll for analysis of more complicated samples. The cell-mediated immunity (CMI) core for the Ul 9 TRIAD grant is already in place at University of Rhode Island and the efforts of Core C with synergize with the efforts of the CMI core.

Public Health Relevance

The COBRE Core C: Cell Analysis and Sorting will serve as a resource for the other COBRE investigators by providing expert advice as they develop and carry out their experiments and provide special instruments for their use in measuring the results of their experiments.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM104317-02
Application #
8727076
Study Section
Special Emphasis Panel (ZGM1-TWD-A)
Project Start
Project End
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
2
Fiscal Year
2014
Total Cost
$69,762
Indirect Cost
$20,151
Name
University of Rhode Island
Department
Type
DUNS #
144017188
City
Kingston
State
RI
Country
United States
Zip Code
02881
Nixon, Christina E; Park, Sangshin; Pond-Tor, Sunthorn et al. (2017) Identification of Protective B-Cell Epitopes within the Novel Malaria Vaccine Candidate Plasmodium falciparum Schizont Egress Antigen 1. Clin Vaccine Immunol 24:
Barbier, Vincent; Lang, Diane; Valois, Sierra et al. (2017) Dengue virus induces mitochondrial elongation through impairment of Drp1-triggered mitochondrial fission. Virology 500:149-160
Li, Ming; Tucker, Lynne D; Asara, John M et al. (2016) Stem-loop binding protein is a multifaceted cellular regulator of HIV-1 replication. J Clin Invest 126:3117-29
Nixon, Christian P (2016) Plasmodium falciparum gametocyte transit through the cutaneous microvasculature: A new target for malaria transmission blocking vaccines? Hum Vaccin Immunother 12:3189-3195
McAllaster, Michael R; Sinclair-Davis, Amy N; Hilton, Nicholas A et al. (2016) A unified approach towards Trypanosoma brucei functional genomics using Gibson assembly. Mol Biochem Parasitol 210:13-21
Li, Ming; Ramratnam, Bharat (2016) Proteomic Characterization of Exosomes from HIV-1-Infected Cells. Methods Mol Biol 1354:311-26
Arsenault, Amanda B; Bliss, Joseph M (2015) Neonatal Candidiasis: New Insights into an Old Problem at a Unique Host-Pathogen Interface. Curr Fungal Infect Rep 9:246-252
Li, Ming (2015) Proteomics in the investigation of HIV-1 interactions with host proteins. Proteomics Clin Appl 9:221-34
McAllaster, Michael R; Ikeda, Kyojiro N; Lozano-Núñez, Ana et al. (2015) Proteomic identification of novel cytoskeletal proteins associated with TbPLK, an essential regulator of cell morphogenesis in Trypanosoma brucei. Mol Biol Cell 26:3013-29
Medin, Carey L; Valois, Sierra; Patkar, Chinmay G et al. (2015) A plasmid-based reporter system for live cell imaging of dengue virus infected cells. J Virol Methods 211:55-62

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