Many nutrient-dependent signaling pathways await discovery. Members of the proposed Nebraska Center for the Prevention of Obesity-related Diseases (NPOD) share the vision that identification of such pathways and their manipulation through dietary intervention has enormous potential to prevent and ameliorate obesity-related diseases, particularly cardiovascular disease, diabetes, and non-alcoholic fatty liver disease (NAFLD). NPOD will support a Molecular Biology, Bioinformatics and Biostatistics Core (MB^C), which will increase the rate of discovery by providing essential analytical and bioinformatics services and developing the workforce in the laboratories of NPOD members. The MB^C's services will focus on tools relevant to the discovery of nutrient-dependent cell signals, making it unique among core facilities in the United States. The MB^C will comprise a (1) Nutrient Signaling Laboratory - with the necessary equipment to offer services identified by NPOD Project Leaders as crucial - adjoining a personnel office and Collaboration Zone and (2) state-of-the-art Communications and Training Portal equipped with audiovisual technology to support inter-campus collaboration and virtual training. The MB^C's toolbox will be expanded in the initial phase of support through a tool development grants program and a research tool development program supported in house. Following the initial period of funding, the Core will expand its toolbox to include assays relevant for other nutrient signaling pathways of interest to other NPOD investigators and external users. To provide comprehensive service, the MB C will be staffed with a Director and two technicians with expertise in nutrient signaling, gene expression, and molecular biology and two Bioinformatics Coordinators who will provide consultation with regard to experimental design, bioinformatics analysis, and assistance preparing statistics sections and power calculations for grant applications and manuscripts. The MB^C will also partially support the Director and a Liaison within the Holland Computing Center to facilitate high-speed, high-capacity data transfer and access and analysis of large datasets. NPOD has gained strong institutional support for the alterations and renovations necessary to establish the MB^C, matching more than 2:1 the NIH funds requested for this purpose.

Public Health Relevance

The Nebraska Center for the Prevention of Obesity Diseases through Dietary Molecules will establish a Molecular Biology, Bioinformatics and Biostatistics Core (MB^C) that focuses on diet-dependent epigenetic marks. This focus will position the Core uniquely among gene expression cores in the United States, while contributing to the Center's mission of preventing obesity-related diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
1P20GM104320-01A1
Application #
8662971
Study Section
Special Emphasis Panel (ZGM1-TWD-C (C1))
Project Start
Project End
Budget Start
2014-08-05
Budget End
2015-05-31
Support Year
1
Fiscal Year
2014
Total Cost
$700,181
Indirect Cost
$236,485
Name
University of Nebraska Lincoln
Department
Type
DUNS #
555456995
City
Lincoln
State
NE
Country
United States
Zip Code
68583
Kim, Jiyoung; Okla, Meshail; Erickson, Anjeza et al. (2016) Eicosapentaenoic Acid Potentiates Brown Thermogenesis through FFAR4-dependent Up-regulation of miR-30b and miR-378. J Biol Chem 291:20551-62
Wang, Hai; Zhao, Miaoyun; Sud, Neetu et al. (2016) Glucagon regulates hepatic lipid metabolism via cAMP and Insig-2 signaling: implication for the pathogenesis of hypertriglyceridemia and hepatic steatosis. Sci Rep 6:32246
Lu, Sizhao; Natarajan, Sathish Kumar; Mott, Justin L et al. (2016) Ceramide Induces Human Hepcidin Gene Transcription through JAK/STAT3 Pathway. PLoS One 11:e0147474
Farris, Eric; Brown, Deborah M; Ramer-Tait, Amanda E et al. (2016) Micro- and nanoparticulates for DNA vaccine delivery. Exp Biol Med (Maywood) 241:919-29
Krumbeck, Janina A; Maldonado-Gomez, Maria X; Ramer-Tait, Amanda E et al. (2016) Prebiotics and synbiotics: dietary strategies for improving gut health. Curr Opin Gastroenterol 32:110-9
Cordonier, Elizabeth L; Jarecke, Sarah K; Hollinger, Frances E et al. (2016) Inhibition of acetyl-CoA carboxylases by soraphen A prevents lipid accumulation and adipocyte differentiation in 3T3-L1 cells. Eur J Pharmacol 780:202-8
Yang, Junyi; Bindels, Laure B; Segura Munoz, Rafael R et al. (2016) Disparate Metabolic Responses in Mice Fed a High-Fat Diet Supplemented with Maize-Derived Non-Digestible Feruloylated Oligo- and Polysaccharides Are Linked to Changes in the Gut Microbiota. PLoS One 11:e0146144
Xie, Fang; Anderson, Christopher L; Timme, Kelsey R et al. (2016) Obesity-Dependent Increases in Oocyte mRNAs Are Associated With Increases in Proinflammatory Signaling and Gut Microbial Abundance of Lachnospiraceae in Female Mice. Endocrinology 157:1630-43
Natarajan, Sathish Kumar; Rasineni, Karuna; Ganesan, Murali et al. (2015) Structure, function and metabolism of hepatic and adipose tissue lipid droplets: implications in alcoholic liver disease. Curr Mol Pharmacol :
Shu, Jiang; Chiang, Kevin; Zempleni, Janos et al. (2015) Computational Characterization of Exogenous MicroRNAs that Can Be Transferred into Human Circulation. PLoS One 10:e0140587

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