Diabetic animal models are essential for studies in all of the COBRE projects and are also often required by other diabetes researchers. However, induction and monitoring of diabetes and maintenance of diabetic animals for long durations are associated with a tremendous amount of routine work. The goal of this Core is to provide a centralized service for induction of diabetes, maintenance and use of diabetic animals and to provide support to the diabetes research. In the first phase of COBRE, we have establistied the diabetic animal core and diabetic animal tissue bank. The Core has provided diabetic animals, tissues, data and technical assistance to multiple Pis. The Core has greatly increased the efficiency of diabetic animal research and has reduced the costs to PJIs and other COBRE members for diabetic animal models. As diabetes research expands in Oklahoma, there is a demand to expand this Core service to serve more diabetes researchers in Oklahoma. In the second phase of the COBRE, we will continue and augment these Core services. 1). To induce diabetes by streptozotocin injection in rats or mice or in transgenic or gene knockout mice as required by investigators. 2). To breed and genotype genetic diabetic mice and rats. 3). To monitor diabetes by measuring hyperglycemia and inject insulin when necessary. 4). To collect and record clinical data from diabetic animals, such as body weight and urine volume. 5). To monitor renal function of diabetic animals by measuring albumin and creatinine concentrations in the urine. 6). To provide special diets for diabetic animals upon request by users. 7). To induce retinal neovascularization in the oxygen-induced retinopathy (OIR) model, a commonly used model for proliferative diabetic retinopathy. 8). To perform specialized assays to evaluate diabetic complications. 9). To dissect tissues and coordinate sharing of animal tissues. This Diabetic Animal Core will assist the PJIs in their projects and reduce their routine work in the induction and maintenance of diabetic animals. The coordinated sharing of diabetic animal tissues will also substantially reduce the budget for animal models.

Public Health Relevance

Diabetes represents a major threat to the health of working age and older populations. More diabetes research is required to understand the pathogenesis of diabetes and its complications and develop new treatments. Most diabetes studies require using diabetic animal models. This core will provide centralized support to researchers using diabetic animal models and thus, will promote diabetes research.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Exploratory Grants (P20)
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University of Oklahoma Health Sciences Center
Oklahoma City
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Chen, Qian; Qiu, Fangfang; Zhou, Kelu et al. (2017) Pathogenic Role of microRNA-21 in Diabetic Retinopathy Through Downregulation of PPAR?. Diabetes 66:1671-1682
Li, Manna; Qian, Ming; Xu, Jian (2017) Vascular Endothelial Regulation of Obesity-Associated Insulin Resistance. Front Cardiovasc Med 4:51
Pearsall, Elizabeth A; Cheng, Rui; Zhou, Kelu et al. (2017) PPAR? is essential for retinal lipid metabolism and neuronal survival. BMC Biol 15:113
Vadvalkar, Shraddha S; Matsuzaki, Satoshi; Eyster, Craig A et al. (2017) Decreased Mitochondrial Pyruvate Transport Activity in the Diabetic Heart: ROLE OF MITOCHONDRIAL PYRUVATE CARRIER 2 (MPC2) ACETYLATION. J Biol Chem 292:4423-4433
Le, Yun-Zheng (2017) VEGF production and signaling in Müller glia are critical to modulating vascular function and neuronal integrity in diabetic retinopathy and hypoxic retinal vascular diseases. Vision Res 139:108-114
Qiu, Fangfang; Liu, Zhen; Zhou, Yueping et al. (2017) Decreased Circulating Levels of Dickkopf-1 in Patients with Exudative Age-related Macular Degeneration. Sci Rep 7:1263
He, Xuemin; Cheng, Rui; Park, Kyoungmin et al. (2017) Pigment epithelium-derived factor, a noninhibitory serine protease inhibitor, is renoprotective by inhibiting the Wnt pathway. Kidney Int 91:642-657
Jung, Dongju; Xu, Yuechi; Sun, Zhongjie (2017) Induction of anti-aging gene klotho with a small chemical compound that demethylates CpG islands. Oncotarget 8:46745-46755
Du, Mei; Phelps, Eric; Balangue, Michael J et al. (2017) Transgenic Mice Over-Expressing RBP4 Have RBP4-Dependent and Light-Independent Retinal Degeneration. Invest Ophthalmol Vis Sci 58:4375–4383
Shin, Younghwa; Moiseyev, Gennadiy; Chakraborty, Dibyendu et al. (2017) A Dominant Mutation in Rpe65, D477G, Delays Dark Adaptation and Disturbs the Visual Cycle in the Mutant Knock-In Mice. Am J Pathol 187:517-527

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