Diabetic animal models are essential for studies in all of the COBRE projects and are also often required by other diabetes researchers. However, induction and monitoring of diabetes and maintenance of diabetic animals for long durations are associated with a tremendous amount of routine work. The goal of this Core is to provide a centralized service for induction of diabetes, maintenance and use of diabetic animals and to provide support to the diabetes research. In the first phase of COBRE, we have establistied the diabetic animal core and diabetic animal tissue bank. The Core has provided diabetic animals, tissues, data and technical assistance to multiple Pis. The Core has greatly increased the efficiency of diabetic animal research and has reduced the costs to PJIs and other COBRE members for diabetic animal models. As diabetes research expands in Oklahoma, there is a demand to expand this Core service to serve more diabetes researchers in Oklahoma. In the second phase of the COBRE, we will continue and augment these Core services. 1). To induce diabetes by streptozotocin injection in rats or mice or in transgenic or gene knockout mice as required by investigators. 2). To breed and genotype genetic diabetic mice and rats. 3). To monitor diabetes by measuring hyperglycemia and inject insulin when necessary. 4). To collect and record clinical data from diabetic animals, such as body weight and urine volume. 5). To monitor renal function of diabetic animals by measuring albumin and creatinine concentrations in the urine. 6). To provide special diets for diabetic animals upon request by users. 7). To induce retinal neovascularization in the oxygen-induced retinopathy (OIR) model, a commonly used model for proliferative diabetic retinopathy. 8). To perform specialized assays to evaluate diabetic complications. 9). To dissect tissues and coordinate sharing of animal tissues. This Diabetic Animal Core will assist the PJIs in their projects and reduce their routine work in the induction and maintenance of diabetic animals. The coordinated sharing of diabetic animal tissues will also substantially reduce the budget for animal models.

Public Health Relevance

Diabetes represents a major threat to the health of working age and older populations. More diabetes research is required to understand the pathogenesis of diabetes and its complications and develop new treatments. Most diabetes studies require using diabetic animal models. This core will provide centralized support to researchers using diabetic animal models and thus, will promote diabetes research.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Exploratory Grants (P20)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Oklahoma Health Sciences Center
Oklahoma City
United States
Zip Code
Hu, Shuqun; Liu, Hua; Ha, Yonju et al. (2015) Posttranslational modification of Sirt6 activity by peroxynitrite. Free Radic Biol Med 79:176-85
McBride, Jeffrey D; Jenkins, Alicia J; Liu, Xiaochen et al. (2014) Elevated circulation levels of an antiangiogenic SERPIN in patients with diabetic microvascular complications impair wound healing through suppression of Wnt signaling. J Invest Dermatol 134:1725-34
Zhou, Kelu Kevin; Benyajati, Siribhinya; Le, Yun et al. (2014) Interruption of Wnt signaling in Müller cells ameliorates ischemia-induced retinal neovascularization. PLoS One 9:e108454
Moran, Elizabeth; Ding, Lexi; Wang, Zhongxiao et al. (2014) Protective and antioxidant effects of PPAR? in the ischemic retina. Invest Ophthalmol Vis Sci 55:4568-76
Fu, Shuhua; Zhu, Meili; Wang, Changyun et al. (2014) Efficient induction of productive Cre-mediated recombination in retinal pigment epithelium. Mol Vis 20:480-7
Takahashi, Yusuke; Moiseyev, Gennadiy; Ma, Jian-xing (2014) Identification of key residues determining isomerohydrolase activity of human RPE65. J Biol Chem 289:26743-51
Lee, Kyungwon; Shin, Younghwa; Cheng, Rui et al. (2014) Receptor heterodimerization as a novel mechanism for the regulation of Wnt/?-catenin signaling. J Cell Sci 127:4857-69
Fu, Suhua; Zhu, Meili; Ash, John D et al. (2014) Investigating the role of retinal Müller cells with approaches in genetics and cell biology. Adv Exp Med Biol 801:401-5
Crosswhite, Patrick; Chen, Kai; Sun, Zhongjie (2014) AAV delivery of tumor necrosis factor-? short hairpin RNA attenuates cold-induced pulmonary hypertension and pulmonary arterial remodeling. Hypertension 64:1141-50
Liu, Hongtao; Wang, Zhongxiao; Yu, Shujie et al. (2014) Proteasomal degradation of O-GlcNAc transferase elevates hypoxia-induced vascular endothelial inflammatory response†. Cardiovasc Res 103:131-9

Showing the most recent 10 out of 20 publications