Core B: Transgenic and Gene Targeting Core The Transgenic and Gene Targeting Core integrates with and enhances the capabilities of our exisitng Transgenic and Gene Targeting Facility with a proven track record of success in creating transgenic and knockout mice. This Core will assist COBRE investigators by providing cost-efffective serviecs for the generation, characterization, and maintenance of genetic mouse models. Ofthe 32 senior scientists in the Center, 9 are frequent users ofthe Core, and 2 of the 5 COBRE Beginning Investigator projects also have plans to utilize this facility. This Core will provide COBRE scientists with the service to microinject DNA into fertilized eggs and transfer embryos to recipient females to create transgenic mice. In addition, this Core will provide services to create chimeric mice, including the maintenance of several ES cell lines, electroporation and selection of ES cells, karyotype determination and blastocyst injection of selected ES cells. COBRE funds will be used to expand these services to include the manipulation and preparation of DNA for microinjection or gene targeting, and genotyping of transgenic and knockout mice. The Core also provides cryopreservation of mouse lines. The COBRE funds will subsidize services for Beginning Investigators and Center members and KUMC will continue to provide significant institutional support for this facility. The Facility is staffed by a Technical Director, Scientific Director, and two Research Associates, who will interface with two COBRE co-advisors

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM104936-07
Application #
8534216
Study Section
Special Emphasis Panel (ZRR1-RI-B)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
7
Fiscal Year
2013
Total Cost
$159,935
Indirect Cost
$54,018
Name
University of Kansas
Department
Type
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160
Tran, Pamela V; Sharma, Madhulika; Li, Xiaogang et al. (2014) Developmental signaling: does it bridge the gap between cilia dysfunction and renal cystogenesis? Birth Defects Res C Embryo Today 102:159-73
Cha, Jeeyeon; Bartos, Amanda; Park, Craig et al. (2014) Appropriate crypt formation in the uterus for embryo homing and implantation requires Wnt5a-ROR signaling. Cell Rep 8:382-92
Pierce, A N; Ryals, J M; Wang, R et al. (2014) Vaginal hypersensitivity and hypothalamic-pituitary-adrenal axis dysfunction as a result of neonatal maternal separation in female mice. Neuroscience 263:216-30
Kumar, T Rajendra (2014) The quest for male germline stem cell markers: PAX7 gets ID'd. J Clin Invest 124:4219-22
Rumi, M A Karim; Dhakal, Pramod; Kubota, Kaiyu et al. (2014) Generation of Esr1-knockout rats using zinc finger nuclease-mediated genome editing. Endocrinology 155:1991-9
Tan, Ee Phie; Villar, Maria T; E, Lezi et al. (2014) Altering O-linked ?-N-acetylglucosamine cycling disrupts mitochondrial function. J Biol Chem 289:14719-30
Saadi, Irfan; Das, Pragnya; Zhao, Minglian et al. (2013) Msx1 and Tbx2 antagonistically regulate Bmp4 expression during the bud-to-cap stage transition in tooth development. Development 140:2697-702
Dasouki, Majed J; Rafi, Syed K; Olm-Shipman, Adam J et al. (2013) Exome sequencing reveals a thrombopoietin ligand mutation in a Micronesian family with autosomal recessive aplastic anemia. Blood 122:3440-9
Taniguchi, Cullen M; Finger, Elizabeth C; Krieg, Adam J et al. (2013) Cross-talk between hypoxia and insulin signaling through Phd3 regulates hepatic glucose and lipid metabolism and ameliorates diabetes. Nat Med 19:1325-30
Abrahamson, Dale R; St John, Patricia L; Stroganova, Larysa et al. (2013) Laminin and type IV collagen isoform substitutions occur in temporally and spatially distinct patterns in developing kidney glomerular basement membranes. J Histochem Cytochem 61:706-18

Showing the most recent 10 out of 11 publications