The Flow Cytometry and Cell Sorter Core Facility, is equipped with a BD LSR II and a BD FACSAria III flow cytometer, which provide the investigators high speed, high resolution, high purity and viability of cell sorting to fit the application of our COBRE investigators. This core facilitates end users on their research involving the study of infectious disease. The overall goal of Flow Cytometry and Cell Sorter Core Facility is to provide various levels of support on comprehensive flow cytometric analysis and sorting, to ensure the success of the Center of Biomedical Research Excellence in the Host-Pathogen Interactions and of our COBRE junior investigators. The establishment of this core will also foster collaboration and innovation among investigators and members of the academic research community in North Dakota. This core will also educate our undergraduate students and graduate students with the application and the current advances in flow cytometry and cell sorter technology by workshop, lectures and lab-based courses. According to the primary goal of ensuring the success of the Center of Biomedical Research Excellence in the Host-Pathogen Interactions and of our COBRE junior investigators, establishment of the Flow Cytometry and Cell Sorter Core is therefore essential to meet the current and future needs of UND and neighboring institutions, and will be of great importance for the overall success of this grant. Therefore, The Flow Cytometry and Cell Sorting Core is planned as a permanent research core in the UND SMHS that we will establish, grow, and transit to independent core over a 15-year period of COBRE support. The major roles of this Flow Cytometry and cell sorting Core Facility are: Role 1: To provide our COBRE and other biomedical investigators with various levels of support on comprehensive flow cytometric analysis and sorting including instrumentation, technical support and consultation. Role 2: To educate faculty, staff, especially graduate students about the application and the current advances in flow cytometry and cell sorter technology.

Public Health Relevance

Currently, there is no established Flow Cytometry and Cell sorter Core Facility available to COBRE investigators at the UND SMHS. This limitation has negatively impacted ongoing research activities for the proposed COBRE investigators and for the other members of the UND SMHS faculty. Therefore the Flow Cytometry and Cell Sorter Core Facility will (1) support the cell sorting needs for all project leaders and other investigators involved in the project, (2) foster collaborations between the scientific community by providing technical support and consultation, (3) educate investigators and their lab members about the application and the current advances in flow cytometry and cell sorter technology

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM113123-03
Application #
9477743
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2018-05-01
Budget End
2019-04-30
Support Year
3
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of North Dakota
Department
Type
DUNS #
102280781
City
Grand Forks
State
ND
Country
United States
Zip Code
58202
Tripathi, Jitendra Kumar; Sharma, Atul; Sukumaran, Pramod et al. (2018) Oxidant sensor cation channel TRPM2 regulates neutrophil extracellular trap formation and protects against pneumoseptic bacterial infection. FASEB J :fj201800605
Basson, Marc D; Wang, Qinggang; Chaturvedi, Lakshmi S et al. (2018) Schlafen 12 Interaction with SerpinB12 and Deubiquitylases Drives Human Enterocyte Differentiation. Cell Physiol Biochem 48:1274-1290
Greenmyer, Jacob R; Gaultney, Robert A; Brissette, Catherine A et al. (2018) Primary Human Microglia Are Phagocytically Active and Respond to Borrelia burgdorferi With Upregulation of Chemokines and Cytokines. Front Microbiol 9:811
Sun, Yuyang; Sukumaran, Pramod; Selvaraj, Senthil et al. (2018) TRPM2 Promotes Neurotoxin MPP+/MPTP-Induced Cell Death. Mol Neurobiol 55:409-420
Bhattacharya, Atrayee; Kumar, Janani; Hermanson, Kole et al. (2018) The calcium channel proteins ORAI3 and STIM1 mediate TGF-? induced Snai1 expression. Oncotarget 9:29468-29483
Quenum Zangbede, Fredice O; Chauhan, Arun; Sharma, Jyotika et al. (2018) Galectin-3 in M2 Macrophages Plays a Protective Role in Resolution of Neuropathology in Brain Parasitic Infection by Regulating Neutrophil Turnover. J Neurosci 38:6737-6750
Chauhan, Arun; Sun, Yuyang; Sukumaran, Pramod et al. (2018) M1 Macrophage Polarization Is Dependent on TRPC1-Mediated Calcium Entry. iScience 8:85-102
Sukumaran, Pramod; Sun, Yuyang; Antonson, Neil et al. (2018) Dopaminergic neurotoxins induce cell death by attenuating NF-?B-mediated regulation of TRPC1 expression and autophagy. FASEB J 32:1640-1652
Zhou, Xikun; Li, Xuefeng; Wu, Min (2018) miRNAs reshape immunity and inflammatory responses in bacterial infection. Signal Transduct Target Ther 3:14
Jondle, Christopher N; Gupta, Kuldeep; Mishra, Bibhuti B et al. (2018) Klebsiella pneumoniae infection of murine neutrophils impairs their efferocytic clearance by modulating cell death machinery. PLoS Pathog 14:e1007338

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