- Interaction of Dietary Potassium with High Dietary Sodium on the Vasculature of Humans Cardiovascular disease remains a major Public Health problem in the U.S. and is the result of sub-clinical diseases such as atherosclerosis and high blood pressure (BP). Several dietary factors have been implicated as risk factors. Indeed, it is well known that excess sodium consumption can increase BP while consumption of potassium has been shown to have BP lowering properties. While the role of these two nutrients on BP is widely accepted, their impact on the vasculature has received less attention. Vascular endothelial dysfunction, characterized by impaired dilation is an important non-traditional risk factor for atherosclerosis. Data in animal models suggest that salt loading, independent of changes in BP, results in vascular endothelial dysfunction while evidence is mounting that potassium may be beneficial to vascular health. Potential mechanisms responsible for sodium induced vascular dysfunction include overproduction of reactive oxygen species and stiffening of endothelial cells resulting in reduced nitric oxide (NO) production/bioavailability. Potassium has been suggested to have antioxidant capabilities that may counteract the effects of high sodium and has been shown to soften cultured endothelial cells stiffened from high sodium. Our central hypothesis is that sodium directly affects the vasculature and simultaneous consumption of potassium can ameliorate the detrimental effects of sodium by reducing oxidative stress and cell stiffness, thereby preserving NO. In this COBRE subproject, we propose to use a 21-day controlled feeding study to compare the effects of a high sodium diet (300 mmol) combined with either a high (120 mmol) or moderate (65 mmol) amount of potassium on 2 levels of the vasculature, conduit and microvasculature. These experiments will be performed in salt-resistant adults to study the vascular effects alone, independent of changes in BP. 24-hour ambulatory BP and urine collections during each diet condition will permit the individual assessment of salt sensitivity of BP. Brachial artery flow- mediated dilation will be used to assess conduit endothelial-dependent dilation and cutaneous vasodilation in response to local heating using laser Doppler flowmetry coupled with intradermal microdialysis will be used to assess microvascular function in the forearm. Arterial stiffness will be assessed by carotid-femoral pulse wave velocity and wave reflection by aortic augmentation index. A venous scraping of endothelial cells will be collected for assessment of oxidative stress and cell stiffness. We expect to demonstrate that high potassium protects the endothelium from the deleterious effects of high sodium by reducing oxidative stress and cell stiffness. These studies are novel in that they will be the first to critically evaluate the role of potassium on vascular function independent of BP and employ a comprehensive assessment of vascular function (conduit artery endothelial function, microvascular function, arterial stiffness, and endothelial cells from human subjects) to study the effect of a high potassium diet in the presence of high sodium. Further, the data collected from this proposal would allow for a larger exploration of this topic through a R01.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM113125-03
Application #
9432533
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2018-03-01
Budget End
2019-02-28
Support Year
3
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of Delaware
Department
Type
DUNS #
059007500
City
Newark
State
DE
Country
United States
Zip Code
19716
Patterson, Freda; Grandner, Michael A; Malone, Susan K et al. (2017) Sleep as a Target for Optimized Response to Smoking Cessation Treatment. Nicotine Tob Res :
Tremblay, Mark S; Aubert, Salomé; Barnes, Joel D et al. (2017) Sedentary Behavior Research Network (SBRN) - Terminology Consensus Project process and outcome. Int J Behav Nutr Phys Act 14:75
Patterson, Freda; Zaslav, David S; Kolman-Taddeo, Diana et al. (2017) Smoking Cessation in Pulmonary Care Subjects: A Mixed Methods Analysis of Treatment-Seeking Participation and Preferences. Respir Care 62:179-192
Nair, Uma S; Patterson, Freda; Rodriguez, Daniel et al. (2017) A telephone-based intervention to promote physical activity during smoking cessation: a randomized controlled proof-of-concept study. Transl Behav Med 7:138-147
Wenner, Megan M; Sebzda, Kelly N; Kuczmarski, Andrew V et al. (2017) ETB receptor contribution to vascular dysfunction in postmenopausal women. Am J Physiol Regul Integr Comp Physiol 313:R51-R57
Lenhart, Clare M; Wiemken, Andrew; Hanlon, Alexandra et al. (2017) Perceived neighborhood safety related to physical activity but not recreational screen-based sedentary behavior in adolescents. BMC Public Health 17:722
Casola, Allison R; Nelson, Deborah B; Patterson, Freda (2017) Sex Differences in Contraception Non-Use Among Urban Adolescents: Risk Factors for Unintended Pregnancy. J Sch Health 87:641-649
Wright, Regina S; Gerassimakis, Constance; Bygrave, Desirée et al. (2017) Dietary Factors and Cognitive Function in Poor Urban Settings. Curr Nutr Rep 6:32-40
Robson, Shannon M; Lozano, Alicia J; Papas, Mia et al. (2017) Food Insecurity and Cardiometabolic Risk Factors in Adolescents. Prev Chronic Dis 14:E110
Perkett, Mackenzie; Robson, Shannon M; Kripalu, Varsha et al. (2017) Characterizing Cardiovascular Health and Evaluating a Low-Intensity Intervention to Promote Smoking Cessation in a Food-Assistance Population. J Community Health 42:605-611

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