There is currently no space on the Dartmouth campus that is appropriate and available to house the two new iTarget shared resources, the Molecular Tools Core (MTC) and the Visualizing Molecular Interactions Core (VMIC). While the MTC and the VMIC have been designed to avoid duplication by leveraging access to other shared resources at Dartmouth and UVM for several technologies, there are a number of new technologies that need to be housed on site in a fully operational facility. These include equipment for parallel testing of protein expression and purification conditions, pilot-scale protein production (MTC), and fluorescence and proximity binding studies (VMIC). There is also a need for office space for staff, including a protein biochemist and a data-analyst, as well as a central location to serve as a meeting place for the iTarget faculty (and their research groups) to seek advice on scientific issues related to protein production or quantification of molecular interactions. The Biochemistry Department at the Geisel School of Medicine has identified approximately 875 gross sq. ft. of contiguous laboratory space to house the cores. However, the space has not been renovated and is inadequate for the proposed activities. Specifically, it does not have cell-culture facilities, a cold-room, or a suitable office. Following extensive consultations with our Core Directors, Facilities personnel, and outside engineering staff, we have designed a renovation plan that will provide fully operational space and resources for the MTC and the VMIC. In addition to providing an outstanding environment for the proposed iTarget Core Facilities, the space has been specifically designed with an open floor plan to permit flexible adaptation to new and evolving Core services. Funds are requested to implement this plan. Technical support and operational oversight for the proposed renovation will be provided by Geisel Facilities staff, working in conjunction with iTarget faculty leadership to ensure that the space is both well-suited to user needs and fully compliant with all regulatory standards.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
1P20GM113132-01
Application #
8813300
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2015-01-01
Budget End
2015-12-31
Support Year
1
Fiscal Year
2016
Total Cost
Indirect Cost
Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
Nasa, Isha; Kettenbach, Arminja N (2018) Coordination of Protein Kinase and Phosphoprotein Phosphatase Activities in Mitosis. Front Cell Dev Biol 6:30
Popelka, Hana; Klionsky, Daniel J; Ragusa, Michael J (2018) An atypical BAR domain protein in autophagy. Autophagy 14:1155-1156
Cabrera, Jorge Ruben; Charron, Audra J; Leib, David A (2018) Neuronal subtype determines HSV-1 Latency-Associated-Transcript (LAT) promoter activity during latency. J Virol :
Olmedillas, Eduardo; Cano, Olga; Martínez, Isidoro et al. (2018) Chimeric Pneumoviridae fusion proteins as immunogens to induce cross-neutralizing antibody responses. EMBO Mol Med 10:175-187
Hvorecny, Kelli L; Dolben, Emily; Moreau-Marquis, Sophie et al. (2018) An epoxide hydrolase secreted by Pseudomonas aeruginosa decreases mucociliary transport and hinders bacterial clearance from the lung. Am J Physiol Lung Cell Mol Physiol 314:L150-L156
Holland, Jack; Pan, Qinxin; Grigoryan, Gevorg (2018) Contact prediction is hardest for the most informative contacts, but improves with the incorporation of contact potentials. PLoS One 13:e0199585
Young, Lorna E; Higgs, Henry N (2018) Focal Adhesions Undergo Longitudinal Splitting into Fixed-Width Units. Curr Biol 28:2033-2045.e5
Kettenbach, Arminja N; Schlosser, Kate A; Lyons, Scott P et al. (2018) Global assessment of its network dynamics reveals that the kinase Plk1 inhibits the phosphatase PP6 to promote Aurora A activity. Sci Signal 11:
Lyons, Scott P; Jenkins, Nicole P; Nasa, Isha et al. (2018) A Quantitative Chemical Proteomic Strategy for Profiling Phosphoprotein Phosphatases from Yeast to Humans. Mol Cell Proteomics 17:2447-2461
Bricio-Moreno, Laura; Sheridan, Victoria H; Goodhead, Ian et al. (2018) Evolutionary trade-offs associated with loss of PmrB function in host-adapted Pseudomonas aeruginosa. Nat Commun 9:2635

Showing the most recent 10 out of 56 publications