This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The objective of this study is to bring glycan-based biomarkers to the forefront to diagnose breast cancer at an early stage. In this two-year study four specific aims have been proposed: (i) Identification of glycan biomarkers in established estrogen receptor-positive and estrogen receptor-negative metastatic or non-metastatic human breast cancer cell lines, and in their conditioned media; (ii) Identification of glycan biomarkers in human breast cancer specimens, nipple aspirate, blood and urine samples from high risk as well as different stages of breast cancer; (iii) Structural characterization of the identified glycan biomarkers from breast cancer specimens; and (iv) Conduct a control study to compare identified prominent breast cancer glycan biomarkers expression in clinical specimens (tissues, blood, and urine samples) from patients and or high risk individuals with colon cancer. The proposed study is highly significant because it has translational potential.
These specific aims have not been changed.This study has actualy began in July 1, 2007 and the projected plan for the Year 1 has been:(1) Acquisition of various human breast cancer cells; (2) isolation and quantification of RNA from each of these cell types; (3) conduct a high-throughput glycotranscriptome analysis following the technology of real-time quantitative RT-PCR (qRT-PCR); (4) collection of breast cancer tissue after the surgery performed in patients at the PR Oncology Hospital; (5) an initial screening of glycotranscriptomes in a selective group of patients with colorectal cancer; and (6) collection of serum samples and nipple aspirate from prospective breast cancer patients for glycan structure analysis by MALDI-TOF as well as Q-TOF MS and MS/MS analysis.
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