EXCEED THE SPACEPROVIDED. The COBRE on Membrane Protein Production and Characterization (COMPPAC) is motivated by the disparity between the critical role of membrane proteins in biology and the well-recognized deficiencies in production and structural characterization of these molecules. We will address this challenge via a suite of five subprojects involving seven Pis from four academic disciplines at all faculty ranks, with all five of the lead Pis being either early-career or junior investigators. The scope of the research spans a spectrumfrom membrane protein overexpression, solubilization and purification, to structure-function studies based on crystallographic characterization, to predominantly functional studies of biomedically significant or related membrane proteins. The membrane proteins to be studied span a variety of structural and functional characteristics, including receptors, adhesion proteins and interfacial enzymes, and their relevance to several biomedically important conditions will be examined, including cancer, spermatogenesis and chemical warfare. The COBRE will have a strong interdisciplinary flavor by virtue of collaborations within and between subprojects, and as the initial subprojects acquire independent support, the scope of the research activities will be expanded by complementary research within several additional active membrane protein research labs. Core facilities support to be provided for protein production, structural biology and bioimaging is closely integrated with the substantial infrastructure for biomolecular research at the University of Delaware and the Delaware Biotechnology Institute. The COBRE will also foster the systematic application of the methods under development to membrane proteins in general, especially for the expression, purification and structural biology studies. This can have a significant impact on the membrane protein research community at large, and on the national infrastructure on membrane protein structural biology being developed within the NIH Roadmap.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR015588-10
Application #
7625189
Study Section
Special Emphasis Panel (ZRR1-RI-8 (01))
Program Officer
Maruvada, Padma
Project Start
2000-09-30
Project End
2010-09-09
Budget Start
2009-06-01
Budget End
2010-09-09
Support Year
10
Fiscal Year
2009
Total Cost
$2,072,350
Indirect Cost
Name
University of Delaware
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
059007500
City
Newark
State
DE
Country
United States
Zip Code
19716
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Cui, Di; Ou, Shu-Ching; Patel, Sandeep (2015) Protein denaturants at aqueous-hydrophobic interfaces: self-consistent correlation between induced interfacial fluctuations and denaturant stability at the interface. J Phys Chem B 119:164-78
Hu, Yuan; Patel, Sandeep (2015) Structural and Thermodynamic Insight into Spontaneous Membrane-Translocating Peptides Across Model PC/PG Lipid Bilayers. J Membr Biol 248:505-15
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