This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The major objective of the proposed research plan is to trace the signal transduction pathways responsible for integrin-mediated upregulation of Bcl-2 transcription and cell survival. I will use cell culture and molecular biology techniques. I hypothesize that integrins mediate cell survival by activating signal transduction pathways that lead to increased Bcl-2 expression. This work has the following specific aims: 1. To investigate the role of known signal transduction proteins in integrin mediated upregulation of bcl-2 expression. 2. To isolate by expression cloning proteins that upregulate bcl-2 transcription (BITs) and determine how they affect integrin-mediated signals. 3. To determine the expression patterns of these BIT proteins. 4. To identify proteins that interact with BITs by yeast two-hybrid analysis. Understanding these pathways will provide the molecular mechanism by which integrins mediate survival signals.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016453-10
Application #
8360589
Study Section
Special Emphasis Panel (ZRR1-RI-5 (01))
Project Start
2011-06-01
Project End
2012-09-23
Budget Start
2011-06-01
Budget End
2012-05-14
Support Year
10
Fiscal Year
2011
Total Cost
$318,719
Indirect Cost
Name
University of Hawaii
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
965088057
City
Honolulu
State
HI
Country
United States
Zip Code
96822
Baumer, Yvonne; McCurdy, Sara; Alcala, Martin et al. (2017) CD98 regulates vascular smooth muscle cell proliferation in atherosclerosis. Atherosclerosis 256:105-114
Pomozi, Viola; Brampton, Christopher; Szeri, Flóra et al. (2017) Functional Rescue of ABCC6 Deficiency by 4-Phenylbutyrate Therapy Reduces Dystrophic Calcification in Abcc6(-/-) Mice. J Invest Dermatol 137:595-602
Doe, Jinger; Kaindl, Angela M; Jijiwa, Mayumi et al. (2017) PTRH2 gene mutation causes progressive congenital skeletal muscle pathology. Hum Mol Genet 26:1458-1464
Yanagihara, Angel Anne; Wilcox, Christie L (2017) Cubozoan Sting-Site Seawater Rinse, Scraping, and Ice Can Increase Venom Load: Upending Current First Aid Recommendations. Toxins (Basel) 9:
Wilcox, Christie L; Headlam, Jasmine L; Doyle, Thomas K et al. (2017) Assessing the Efficacy of First-Aid Measures in Physalia sp. Envenomation, Using Solution- and Blood Agarose-Based Models. Toxins (Basel) 9:
Yanagihara, Angel A; Wilcox, Christie; King, Rebecca et al. (2016) Experimental Assays to Assess the Efficacy of Vinegar and Other Topical First-Aid Approaches on Cubozoan (Alatina alata) Tentacle Firing and Venom Toxicity. Toxins (Basel) 8:
Hartmann, Bianca; Wai, Timothy; Hu, Hao et al. (2016) Homozygous YME1L1 mutation causes mitochondriopathy with optic atrophy and mitochondrial network fragmentation. Elife 5:
Hoffmann, Peter R; Panigada, Maddalena; Soprana, Elisa et al. (2015) Preclinical development of HIvax: Human survivin highly immunogenic vaccines. Hum Vaccin Immunother 11:1585-95
Rose, Aaron H; Hoffmann, FuKun W; Hara, Jared H et al. (2015) Adjuvants may reduce in vivo transfection levels for DNA vaccination in mice leading to reduced antigen-specific CD8+ T cell responses. Hum Vaccin Immunother 11:2305-11
Cabrera-Fuentes, Hector A; Lopez, Mercedes L; McCurdy, Sara et al. (2015) Regulation of monocyte/macrophage polarisation by extracellular RNA. Thromb Haemost 113:473-81

Showing the most recent 10 out of 114 publications