This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Maintain and Expand Shared Research Facilities An integrated statewide system of Research facilities that includes three Research Core Laboratories, statewide Bioinformatics Facilities, and a Research Vivarium was developed and enhanced by BRIN/INBRE-1 and will be maintained and strengthened. Access to these shared resources will be facilitated and full-time expert technical assistance will be provided. User fees will be waived or paid for INBRE faculty and students. The cost of reagents and other materials will be met by the investigator through small faculty development seed grants so that faculty, postdocs, or students need only provide funds for consumable supplies. Additionally, researchers from all the Western IDeA states will be invited to use these facilities University of Idaho A. Microarray Facility 1. Biopick robot 2. BioRobotics 3000 workstation 3. MicroGrid Robot 4. GSM417 arrayer 5. GenePix 4000a scanner 6. BioRad pulsed-gel electophoresis 7. High throughput PCS 8. Real-time PCR B. Imaging Core: 1. IVIS 200 (In vitro Imaging System) 2. Confocal BioRad 1020 (upgrades and software) 3. FACS (Fluorescence Activated Cell Sorter) C. Bioinformatics Core: 1. Access Grid Node classrooms 2. Computer labs 3. Cluster computers 4. Robotic backup 5. Servers Idaho State University D. MCRF (Molecular Core Research Facility): 1. MCRF ABI DNA sequencer service contract 2. MCRF QPCR upgrade 3. Microscope/vessel chambers 4. MRCF Tissue processor Boise State University E. Proteomics Core 1. Gyromax 737R Incubator Shaker 2. Gyromax 737 Incubator Shaker 3. Mass Spectrometer (LCQ DECA XP Plus). Mass Spectrometer equipment purchased in the following components: i. Mass Spectrometer/Protemomex Wo ii. Toshiba 1400 XL Plus Series 6 iii. Proteomex workstation 4. NanoDrop Technologies ND-1000 5. Protean IEF System 6. BioRad External LASER Molecular Imager 7. Silicon Graphics Imaging Computer 8. Eppendorf Vacufuge Concentrator 5301 9. ALC Refrigerated Centrifuge 10. Nuaire Hood x 2 11. Axiovert Tissue Culture Microscop 12. SPM 1000HV Control Electronics, AFM 100 AFM Interface module for use with RHK Control systems, AIM-MI control interface for Molecular Imaging and DI (PicoSPM &Multinode) 13. Coulter Epics XL Flow Cytometer 14. Jouan Refrigerated Centrifuge 15. Digital Instruments (Veeco Metrology group) Multimode PicoForce system 16. OLIS DM 45k Spectroflurimetery system. The following components were included: i. Stop-flow accessory ?Classic Model ii. Varian Spectrophotometer 17. Cary 50 Microplate reader 18. Cary 50 Fiber-optic microprobe 10 mm path 19. Dell OptiPlex GX260 computers 20. ABIT 7300 PCR Themocycler F. Instrumentations Shop Shared Facilities 1. LSM 510 Meta 405 with Axio observer Z1 Confocal Imaging System 2. BioTek Microplate Washer 3. Kodak IS4000R Image Station 4. Leica CM1950 OUVVM cryostat

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016454-11
Application #
8359676
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2011-04-01
Project End
2012-03-31
Budget Start
2011-04-01
Budget End
2012-03-31
Support Year
11
Fiscal Year
2011
Total Cost
$87,273
Indirect Cost
Name
University of Idaho
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
075746271
City
Moscow
State
ID
Country
United States
Zip Code
83844
Bowman, Kole; Rose, Jack (2017) Estradiol stimulates glycogen synthesis whereas progesterone promotes glycogen catabolism in the uterus of the American mink (Neovison vison). Anim Sci J 88:45-54
Karmakar, Saswata; Guenther, Dale C; Gibbons, Bradley C et al. (2017) Recognition of mixed-sequence DNA using double-stranded probes with interstrand zipper arrangements of O2'-triphenylene- and coronene-functionalized RNA monomers. Org Biomol Chem 15:9362-9371
Simmons, Aaron B; Bloomsburg, Samuel J; Sukeena, Joshua M et al. (2017) DSCAM-mediated control of dendritic and axonal arbor outgrowth enforces tiling and inhibits synaptic plasticity. Proc Natl Acad Sci U S A 114:E10224-E10233
Marx, Hannah E; Dentant, C├ędric; Renaud, Julien et al. (2017) Riders in the sky (islands): using a mega-phylogenetic approach to understand plant species distribution and coexistence at the altitudinal limits of angiosperm plant life. J Biogeogr 44:2618-2630
Olson, Xiaoping; Kotani, Shohei; Padilla, Jennifer E et al. (2017) Availability: A Metric for Nucleic Acid Strand Displacement Systems. ACS Synth Biol 6:84-93
Yano, Hirokazu; Wegrzyn, Katarznya; Loftie-Eaton, Wesley et al. (2016) Evolved plasmid-host interactions reduce plasmid interference cost. Mol Microbiol 101:743-56
Gunderson, Mark P; Pickett, Melissa A; Martin, Justin T et al. (2016) Variations in hepatic biomarkers in American alligators (Alligator mississippiensis) from three sites in Florida, USA. Chemosphere 155:180-187
Aldape, Michael J; Bayer, Clifford R; Bryant, Amy E et al. (2016) A novel murine model of Clostridium sordellii myonecrosis: Insights into the pathogenesis of disease. Anaerobe 38:103-110
Simmons, Aaron B; Bloomsburg, Samuel J; Billingslea, Samuel A et al. (2016) Pou4f2 knock-in Cre mouse: A multifaceted genetic tool for vision researchers. Mol Vis 22:705-17
Fernandes, K A; Bloomsburg, S J; Miller, C J et al. (2016) Novel axon projection after stress and degeneration in the Dscam mutant retina. Mol Cell Neurosci 71:1-12

Showing the most recent 10 out of 466 publications