This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The objective of this project is to use Saccharomyces cerevisiae (budding yeast) and human cancer cell lines to identify the molecular target(s) of a putative anti-cancer drug, fusarochromanone (FC-101a). FC-101a is a mycotoxin produced by Fusarium equiseti, which causes plant diseases such as wilt, scab, and rot. Studies performed at the National Cancer Institute revealed that FC-101a is a potent inhibitor of 35 human cancer cell lines. Recent studies indicate that FC-101a increases apoptosis in melanoma cell lines and significantly reduces tumor growth in mice. To understand the molecular mechanisms by which this drug acts to elicit its anti-tumor effects, we performed a genetic screen of the yeast deletion collection, which revealed that a YCA1 gene deletion strain is hypersensitive to FC-101a. YCA1 encodes the yeast metacaspase that initiates an apoptosis-like response and participates in the mitotic G2/M checkpoint. We hypothesize that FC-101a may target gene products that regulate cell cycle progression and/or apoptosis. Currently, we are classifying genes that are differentially expressed in yeast and a bladder cancer cell line with greater than 70% growth inhibition after treatment with 100 nM FC-101a. From this cell line we have identified genes that are differentially expressed after 24, 48 and 72 hours of FC-101a treatment. Future studies will involve isolating proteins that interact with FC-101a. Putative molecular targets of FC-101a identified in yeast and human cancer cell lines will be validated in animal model studies.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Exploratory Grants (P20)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-RI-7 (01))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Louisiana State University A&M Col Baton Rouge
Schools of Veterinary Medicine
Baton Rouge
United States
Zip Code
El-Saadi, Madison Wynne; Williams-Hart, Tara; Salvatore, Brian A et al. (2015) Use of in-silico assays to characterize the ADMET profile and identify potential therapeutic targets of fusarochromanone, a novel anti-cancer agent. In Silico Pharmacol 3:6
Babu, Sainath; Uppu, Sannihith N; Martin, Brittany et al. (2015) Unusually high levels of bisphenol A (BPA) in thermal paper cash register receipts (CRs): development and application of a robust LC-UV method to quantify BPA in CRs. Toxicol Mech Methods 25:410-6
Ibrahim, Sulaimon; Chowriappa, Pradeep; Dua, Sumeet et al. (2015) Classification of diabetes maculopathy images using data-adaptive neuro-fuzzy inference classifier. Med Biol Eng Comput 53:1345-60
Gu, Ying; Barzegar, Mansoureh; Chen, Xin et al. (2015) Fusarochromanone-induced reactive oxygen species results in activation of JNK cascade and cell death by inhibiting protein phosphatases 2A and 5. Oncotarget 6:42322-33
Pogue, A I; Dua, P; Hill, J M et al. (2015) Progressive inflammatory pathology in the retina of aluminum-fed 5xFAD transgenic mice. J Inorg Biochem 152:206-9
Zhang, Cheng; Rissman, Robert A; Feng, June (2015) Characterization of ATP alternations in an Alzheimer's disease transgenic mouse model. J Alzheimers Dis 44:375-8
Pasluosta, Cristian F; Chiu, Alan W L (2015) Modulation of grasping force in prosthetic hands using neural network-based predictive control. Methods Mol Biol 1260:179-94
McFerrin, Harris E; Olson, Scott D; Gutschow, Miriam V et al. (2014) Rapidly self-renewing human multipotent marrow stromal cells (hMSC) express sialyl Lewis X and actively adhere to arterial endothelium in a chick embryo model system. PLoS One 9:e105411
Fontana, Juan M; Chiu, Alan W L (2014) Analysis of electrode shift effects on wavelet features embedded in a myoelectric pattern recognition system. Assist Technol 26:71-80
Zheng, Shilong; Zhong, Qiu; Xi, Yulan et al. (2014) Modification and biological evaluation of thiazole derivatives as novel inhibitors of metastatic cancer cell migration and invasion. J Med Chem 57:6653-67

Showing the most recent 10 out of 175 publications