This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The emerging fields of metagenomics and proteomics are significantly transforming our understanding of the roles microorganisms play in all ecosystems, from the human biome to toxin laden environments. Microbiology is no longer restricted to analysis of pure cultures of one species, isolated from natural habitats and interactions with other microflora. The long range goal of this investigation is to assess the microbial community of a complex, environmentally-stressed site within Lamoille County, Vermont. The Vermont Asbestos Group Mine site, located in Lowell and Eden is impacting both the environmental and human health of the region (Levy, 2008;Vermont Dept of Health, 2008). Water and stream bed quality are contaminated with heavy metals, elevated pH levels, and asbestos-laden mine tailings. an A health advisory indicates increased potential risk to humans of asbestos-related illnesses. The objectives of the project are to (a) construct a metagenomic library of community DNA from an impacted aquatic environment at the Mine site, (b) create a cDNA library of expressed genes of the microorganisms inhabiting the site, and (c) isolate total protein from the microbial community for future proteomic analysis. This integrative approach will enable us to address the dynamic roles microorganisms play in complex, human-impacted environments.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016462-10
Application #
8360434
Study Section
Special Emphasis Panel (ZRR1-RI-7 (01))
Project Start
2011-06-01
Project End
2012-05-31
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
10
Fiscal Year
2011
Total Cost
$30,880
Indirect Cost
Name
University of Vermont & St Agric College
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
066811191
City
Burlington
State
VT
Country
United States
Zip Code
05405
Spritzer, M D; Curtis, M G; DeLoach, J P et al. (2016) Sexual interactions with unfamiliar females reduce hippocampal neurogenesis among adult male rats. Neuroscience 318:143-56
Hinkle, Karen L; Anderson, Chad C; Forkey, Blake et al. (2016) Exposure to the lampricide 3-trifluoromethyl-4-nitrophenol results in increased expression of carbohydrate transporters in Saccharomyces cerevisiae. Environ Toxicol Chem 35:1727-32
Nock, Adam M; Wargo, Matthew J (2016) Choline Catabolism in Burkholderia thailandensis Is Regulated by Multiple Glutamine Amidotransferase 1-Containing AraC Family Transcriptional Regulators. J Bacteriol 198:2503-14
Symeonides, Menelaos; Murooka, Thomas T; Bellfy, Lauren N et al. (2015) HIV-1-Induced Small T Cell Syncytia Can Transfer Virus Particles to Target Cells through Transient Contacts. Viruses 7:6590-603
Xie, Yi; Jin, Yu; Merenick, Bethany L et al. (2015) Phosphorylation of GATA-6 is required for vascular smooth muscle cell differentiation after mTORC1 inhibition. Sci Signal 8:ra44
Case, Laure K; Wall, Emma H; Osmanski, Erin E et al. (2015) Copy number variation in Y chromosome multicopy genes is linked to a paternal parent-of-origin effect on CNS autoimmune disease in female offspring. Genome Biol 16:28
Perkins, Timothy N; Peeters, Paul M; Shukla, Arti et al. (2015) Indications for distinct pathogenic mechanisms of asbestos and silica through gene expression profiling of the response of lung epithelial cells. Hum Mol Genet 24:1374-89
Kirshenbaum, Ari; Green, John; Fay, Michael et al. (2015) Reinforcer devaluation as a consequence of acute nicotine exposure and withdrawal. Psychopharmacology (Berl) 232:1583-94
Bentley, P A; Wall, E H; Dahl, G E et al. (2015) Responses of the mammary transcriptome of dairy cows to altered photoperiod during late gestation. Physiol Genomics 47:488-99
Mireault, Gina C; Crockenberg, Susan C; Sparrow, John E et al. (2015) Laughing matters: Infant humor in the context of parental affect. J Exp Child Psychol 136:30-41

Showing the most recent 10 out of 173 publications