This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The cyclin D gene is an important regulator of cell division and differentiation. In this project, """"""""cis-regulatory"""""""" DNA regions that control when and where cyclin D is activated during embryonic development of the purple sea urchin Strongylocentrotus purpuratus are being analyzed to determine their specific roles. Sea urchins and vertebrates share a common ancestor, so knowledge gained from this study can be used to understand aspects of human biology. In humans, cyclin D genes are mis-regulated in certain cancers and neurodegenerative diseases, so this research has health implications. In the current project, potential cis-regulatory regions have been identified computationally based on their similarity to regions in the cyclin D gene of the sea urchin Lytechinus variegatus, as well as by various additional criteria. These candidate cis-regulatory regions were incorporated individually into a """"""""reporter"""""""" transgene encoding green fluorescent protein (GFP), for microinjection into fertilized sea urchin eggs. As the injected embryos develop, GFP expression can be studied by microscopy to determine where in the embryo the cis-regulatory region of interest exerts its action, and quantitatively, by a technique termed Real-Time PCR. From these methods, we have isolated two regions that regulate the activation of the cyclin D gene in the purple sea urchin. These regions are being broken down further, by mutating subregions hypothesized to be required for regulatory function, then re-examining the abilities of the altered regions to induce activation of GFP.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016463-11
Application #
8360317
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2011-05-01
Project End
2012-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
11
Fiscal Year
2011
Total Cost
$41,973
Indirect Cost
Name
Mount Desert Island Biological Lab
Department
Type
DUNS #
077470003
City
Salsbury Cove
State
ME
Country
United States
Zip Code
04672
Hahn, Mark E; Karchner, Sibel I; Merson, Rebeka R (2017) Diversity as Opportunity: Insights from 600 Million Years of AHR Evolution. Curr Opin Toxicol 2:58-71
Ariyachet, Chaiyaboot; Bei├čel, Christian; Li, Xiang et al. (2017) Post-translational modification directs nuclear and hyphal tip localization of Candida albicans mRNA-binding protein Slr1. Mol Microbiol 104:499-519
Nickerson, Chelsea A; Brown, Alexandra L; Yu, Waylin et al. (2017) Prenatal choline supplementation attenuates MK-801-induced deficits in memory, motor function, and hippocampal plasticity in adult male rats. Neuroscience 361:116-128
Wirth, Peter; Yu, Waylin; Kimball, Amanda L et al. (2017) New method to induce mild traumatic brain injury in rodents produces differential outcomes in female and male Sprague Dawley rats. J Neurosci Methods 290:133-144
Mangiamele, Lisa A; Gomez, Julia R; Curtis, Nancy J et al. (2017) GPER/GPR30, a membrane estrogen receptor, is expressed in the brain and retina of a social fish (Carassius auratus) and colocalizes with isotocin. J Comp Neurol 525:252-270
Dickinson, Patsy S; Qu, Xuan; Stanhope, Meredith E (2016) Neuropeptide modulation of pattern-generating systems in crustaceans: comparative studies and approaches. Curr Opin Neurobiol 41:149-157
Palopoli, Michael F; Peden, Colin; Woo, Caitlin et al. (2015) Natural and experimental evolution of sexual conflict within Caenorhabditis nematodes. BMC Evol Biol 15:93
Dickinson, Patsy S; Calkins, Andrew; Stevens, Jake S (2015) Related neuropeptides use different balances of unitary mechanisms to modulate the cardiac neuromuscular system in the American lobster, Homarus americanus. J Neurophysiol 113:856-70
Dickinson, Patsy S; Sreekrishnan, Anirudh; Kwiatkowski, Molly A et al. (2015) Distinct or shared actions of peptide family isoforms: I. Peptide-specific actions of pyrokinins in the lobster cardiac neuromuscular system. J Exp Biol 218:2892-904
McCall, Nora; Mahadevia, Darshini; Corriveau, Jennifer A et al. (2015) Adult emotionality and neural plasticity as a function of adolescent nutrient supplementation in male rats. Pharmacol Biochem Behav 132:125-135

Showing the most recent 10 out of 244 publications