This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The bacterium Staphylococcus aureus is a serious human pathogen and a public health concern in both community and clinical settings. Bacterial resistance to antimicrobial agents, however, reduces chemotherapeutic effectiveness against infectious diseases caused by S. aureus, and in particular, multi-drug resistant strains of this bacterium. Thus, knowledge of such resistance mechanisms would be of clinical utility in the efforts to control the mechanisms that confer resistance, thus restoring the usefulness of chemotherapeutics. The long term objectives of the experiments proposed in this project application are to enhance our understanding of the functional roles of the multidrug efflux pump operon and its transcriptional regulatory system in clinical isolates of methicillin resistant S. aureus (MRSA) and vancomycin intermediate S. aureus (VISA). The central hypotheses are that farABC-encoded efflux pumps mediate resistance to fusidic acid and ethidium, provide scaffolding for clinical resistance to arise, and that yycFG elements control transcription of these pump genes, thus contributing to antimicrobial drug resistance. The rationale for these hypotheses is that once the relationships are known between the multidrug efflux pumps and their transcriptional control systems, then insight will be gained regarding Staphylococcal resistance mechanisms to multiple antimicrobial agents. The expected outcomes are that the training efforts will be facilitated for undergraduates and graduates plus postdoctoral fellows. Physiological studies with transcriptional regulation will provide molecular insight into important systems for bacterial resistance to antimicrobial agents in a serious human pathogen that is a public health concern in both community and clinical settings.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016480-11
Application #
8359749
Study Section
Special Emphasis Panel (ZRR1-RI-4 (01))
Project Start
2011-03-01
Project End
2012-02-29
Budget Start
2011-03-01
Budget End
2012-02-29
Support Year
11
Fiscal Year
2011
Total Cost
$147,903
Indirect Cost
Name
New Mexico State University Las Cruces
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
173851965
City
Las Cruces
State
NM
Country
United States
Zip Code
88003
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