This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Lipidomics Core is in full operation and is providing the following support to COBRE project investigators: (i) expertise in sphingolipid chemistry, analysis and biology, (ii) assistance with experimental design and interpretation of analytical data, (iii) qualitative and quantitative analysis of key sphingolipids from biological samples: cells, tissue, serum and media. Analytical approach is based on High Performance Liquid Chromatography-Tandem Mass spectrometry (LC-MS/MS) technique. Currently, core provides metabolomic profiling of ~ 100 different lipid species including: sphingosine, dihydrosphingosine, phytosphingosine and their phosphates, ceramides, alpha-hydroxy-ceramides, dihydroceramides, phytoceramides, alpha-hydroxy-phytoceramides and their phosphates, sphingomyelin, hexosyl-ceramide, lactosyl ceramide and diacyl-glycerol (DAG's) components. Additionally, a separate analysis of glucosylceramide and galactosylceramide molecular species by SCF-MS/MS approach is also provided. (iv) homogenic synthetic sphingolipids and their analogs for use in cellular, in vitro and in vivo studies. Currently, core provides stereoisomers of modified sphingoid bases, ceramides and their phosphates, sphingomyelin, modulators of sphingolipid metabolism and advanced molecular probes: functionalized, fluorescent, side-specific radioactive sphingolipids and 17C-backbone sphingolipids. Additionally, analytical approach to study sphingolipid metabolism and enzymatic activities (using sphingolipids composed of 17C-backbone and MS analysis) is also available for the COBRE investigators. Starting from July, 2010 up to April 06, 2011, the analytical subCore performed 2612 analyses for the current COBRE projects (analytical limit established for each project was estimated as 300 analyses/year for each project) and 477 analyses for the former COBRE graduates: Dr. Argraves (87), Dr. Hammad (66), Dr. Hama (44) and Dr. Gudz (280).

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR017677-10
Application #
8360378
Study Section
Special Emphasis Panel (ZRR1-RI-5 (01))
Project Start
2011-07-01
Project End
2012-07-18
Budget Start
2011-07-01
Budget End
2012-07-18
Support Year
10
Fiscal Year
2011
Total Cost
$361,354
Indirect Cost
Name
Medical University of South Carolina
Department
Biochemistry
Type
Schools of Medicine
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425
Alexaki, Aikaterini; Clarke, Benjamin A; Gavrilova, Oksana et al. (2017) De Novo Sphingolipid Biosynthesis Is Required for Adipocyte Survival and Metabolic Homeostasis. J Biol Chem 292:3929-3939
Hao, Limin; Ben-David, Oshrit; Babb, Suzann M et al. (2017) Clozapine Modulates Glucosylceramide, Clears Aggregated Proteins, and Enhances ATG8/LC3 in Caenorhabditis elegans. Neuropsychopharmacology 42:951-962
Pulkoski-Gross, Michael J; Uys, Joachim D; Orr-Gandy, K Alexa et al. (2017) Novel sphingosine kinase-1 inhibitor, LCL351, reduces immune responses in murine DSS-induced colitis. Prostaglandins Other Lipid Mediat 130:47-56
Zunke, Friederike; Moise, Alexandra C; Belur, Nandkishore R et al. (2017) Reversible Conformational Conversion of ?-Synuclein into Toxic Assemblies by Glucosylceramide. Neuron :
Hammad, Samar M; Baker, Nathaniel L; El Abiad, Jad M et al. (2017) Increased Plasma Levels of Select Deoxy-ceramide and Ceramide Species are Associated with Increased Odds of Diabetic Neuropathy in Type 1 Diabetes: A Pilot Study. Neuromolecular Med 19:46-56
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Fan, Jie; Wu, Bill X; Crosson, Craig E (2016) Suppression of Acid Sphingomyelinase Protects the Retina from Ischemic Injury. Invest Ophthalmol Vis Sci 57:4476-84
Taguchi, Yoshimitsu; Allende, Maria L; Mizukami, Hiroki et al. (2016) Sphingosine-1-phosphate Phosphatase 2 Regulates Pancreatic Islet ?-Cell Endoplasmic Reticulum Stress and Proliferation. J Biol Chem 291:12029-38
Mazzulli, Joseph R; Zunke, Friederike; Isacson, Ole et al. (2016) ?-Synuclein-induced lysosomal dysfunction occurs through disruptions in protein trafficking in human midbrain synucleinopathy models. Proc Natl Acad Sci U S A 113:1931-6
Podbielska, Maria; Szulc, Zdzis?aw M; Kurowska, Ewa et al. (2016) Cytokine-induced release of ceramide-enriched exosomes as a mediator of cell death signaling in an oligodendroglioma cell line. J Lipid Res 57:2028-2039

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