This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The absence of the Wnt signaling in the canonical pathway is shown to de-repress a cascade of target genes, some of which are implicated to be able to induce or suppress the formation of the forebrain, the midbrain and hindbrain boundary (MHB), the hindbrain, and eyes. Both the brain and eyes (particularly the retinas) are believed to be autonomous circadian oscillators and many key circadian clock genes are rhythmically expressed in the brain and eyes in a robust fashion in zebrafish (Danio rerio). Yet, little is known about how the Wnt signaling regulates circadian rhythmicity and the presumable Wnt signaling target genes that may control daily oscillations of the circadian clock genes. masterblind (mbl) is an ethyl nitrosourea (ENU)-induced mutant generated in a large-scale zebrafish mutagenesis. Zebrafish embryos homozygous for the masterblind (mbl) mutation exhibit reduced or absent eyes and telencephalon, and the expansion of the diencephalic fates to the front of the brain. A mutation in the GSK3-binding domain of zebrafish axin1, a scaffolding protein in the Wnt signaling pathway, results in the eyeless mbl phenotype. It appears that the repression of axin1 and therefore unknown Wnt target genes during the normal development secures the eye formation. In this proposed study, we plan to determine whether mbl disrupts the normal oscillations of circadian clock genes in the retinas and to identify the presumable Wnt target genes that may controls the circadian regulation using microarrays.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR017703-05
Application #
7381945
Study Section
Special Emphasis Panel (ZRR1-RI-A (01))
Project Start
2006-07-01
Project End
2007-06-30
Budget Start
2006-07-01
Budget End
2007-06-30
Support Year
5
Fiscal Year
2006
Total Cost
$64,485
Indirect Cost
Name
University of Oklahoma Health Sciences Center
Department
Ophthalmology
Type
Schools of Medicine
DUNS #
878648294
City
Oklahoma City
State
OK
Country
United States
Zip Code
73117
Bhatti, Faizah; Kung, Johannes W; Vieira, Frederico (2018) Retinal degeneration mutation in Sftpa1tm1Kor/J and Sftpd -/- targeted mice. PLoS One 13:e0199824
Vieira, Frederico; Kung, Johannes W; Bhatti, Faizah (2017) Structure, genetics and function of the pulmonary associated surfactant proteins A and D: The extra-pulmonary role of these C type lectins. Ann Anat 211:184-201
Simón, María Victoria; Agnolazza, Daniela L; German, Olga Lorena et al. (2016) Synthesis of docosahexaenoic acid from eicosapentaenoic acid in retina neurons protects photoreceptors from oxidative stress. J Neurochem 136:931-46
Stiles, Megan; Qi, Hui; Sun, Eleanor et al. (2016) Sphingolipid profile alters in retinal dystrophic P23H-1 rats and systemic FTY720 can delay retinal degeneration. J Lipid Res 57:818-31
Bennett, Lea D; Anderson, Robert E (2016) Current Progress in Deciphering Importance of VLC-PUFA in the Retina. Adv Exp Med Biol 854:145-51
Ding, Xi-Qin; Thapa, Arjun; Ma, Hongwei et al. (2016) The B3 Subunit of the Cone Cyclic Nucleotide-gated Channel Regulates the Light Responses of Cones and Contributes to the Channel Structural Flexibility. J Biol Chem 291:8721-34
Ma, Hongwei; Ding, Xi-Qin (2016) Thyroid Hormone Signaling and Cone Photoreceptor Viability. Adv Exp Med Biol 854:613-8
Cai, Xue; Chen, Lijuan; McGinnis, James F (2015) Correlation of ER stress and retinal degeneration in tubby mice. Exp Eye Res 140:130-138
Bhatti, Faizah; Ball, Genevieve; Hobbs, Ronald et al. (2015) Pulmonary surfactant protein a is expressed in mouse retina by Müller cells and impacts neovascularization in oxygen-induced retinopathy. Invest Ophthalmol Vis Sci 56:232-42
Ding, Xi-Qin; Matveev, Alexander; Singh, Anil et al. (2014) Exploration of cone cyclic nucleotide-gated channel-interacting proteins using affinity purification and mass spectrometry. Adv Exp Med Biol 801:57-65

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