This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The primary goal of project is to understand the mechanisms of sex differentiation in primordial germ cells (PGCs), the embryonic cells that give rise to either sperm or egg cells in the adult. Current evidence suggests that PGCs in both female (XX) and male (XY) embryos begin life being bipotent, capable of differentiating along either the male or female pathway. The decision to differentiate to male or female germs cells is controlled by the gonadal somatic cells, the cells that surround the PGCs. But this has not been fully tested. After sex differentiation, male and female germ cells alter their DNA and begin cell division based on whether they have entered the male or female specific pathway. These sex-specific events are essential to produce functional sperm and eggs. Otherwise, normal fertilization and embryonic development do not occur correctly. However, we do not know the exact relationship between sex differentiation and the following sex-specific events in fetal germ cells. Our previous data demonstrate that in mice by 13.5 days after fertilization, PGCs control their own fate, and are no longer regulated by the surrounding somatic cells. In this project, we will first examine the relationship between sex differentiation and the following sex-specific events in germ cells cultured with external chemicals/factors. Next, we will test which conditions, if any, are required to direct PGCs into male or female pathway. Finally, we will determine if germ cells maintain intrinsic clock to enter meiosis by themselves in the absence of somatic support.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR024206-04
Application #
8360320
Study Section
Special Emphasis Panel (ZRR1-RI-2 (01))
Project Start
2011-07-01
Project End
2012-06-30
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
4
Fiscal Year
2011
Total Cost
$222,056
Indirect Cost
Name
University of Hawaii
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
965088057
City
Honolulu
State
HI
Country
United States
Zip Code
96822
Goh, William A; Zalud, Ivica (2016) Placenta accreta: diagnosis, management and the molecular biology of the morbidly adherent placenta. J Matern Fetal Neonatal Med 29:1795-800
Riches, Zoe; Abanda, Ngu; Collier, Abby C (2015) BCRP protein levels do not differ regionally in adult human livers, but decline in the elderly. Chem Biol Interact 242:203-10
Collier, Abby C; Thévenon, Audrey D; Goh, William et al. (2015) Placental profiling of UGT1A enzyme expression and activity and interactions with preeclampsia at term. Eur J Drug Metab Pharmacokinet 40:471-80
Rose, Aaron H; Hoffmann, FuKun W; Hara, Jared H et al. (2015) Adjuvants may reduce in vivo transfection levels for DNA vaccination in mice leading to reduced antigen-specific CD8+ T cell responses. Hum Vaccin Immunother 11:2305-11
Sato, Brittany L; Ward, Monika A; Astern, Joshua M et al. (2015) Validation of murine and human placental explant cultures for use in sex steroid and phase II conjugation toxicology studies. Toxicol In Vitro 29:103-12
Li, Zicong; Zeng, Fang; Meng, Fanming et al. (2014) Generation of transgenic pigs by cytoplasmic injection of piggyBac transposase-based pmGENIE-3 plasmids. Biol Reprod 90:93
Bertino, Pietro; Urschitz, Johann; Hoffmann, Fukun W et al. (2014) Vaccination with a piggyBac plasmid with transgene integration potential leads to sustained antigen expression and CD8(+) T cell responses. Vaccine 32:1670-7
Dewitt, J; Ochoa, V; Urschitz, J et al. (2014) Constitutively active TrkB confers an aggressive transformed phenotype to a neural crest-derived cell line. Oncogene 33:977-85
Comptour, Aurélie; Moretti, Charlotte; Serrentino, Maria-Elisabetta et al. (2014) SSTY proteins co-localize with the post-meiotic sex chromatin and interact with regulators of its expression. FEBS J 281:1571-84
Vernet, Nadège; Mahadevaiah, Shantha K; Yamauchi, Yasuhiro et al. (2014) Mouse Y-linked Zfy1 and Zfy2 are expressed during the male-specific interphase between meiosis I and meiosis II and promote the 2nd meiotic division. PLoS Genet 10:e1004444

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