This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Flow Cytometry Core will provide expertise in hematopoietic stem cell separations, microvesicle sorting, and cell phenotyping analysis. The Core will be headed by Mr. Mark Dooner. Mr. Dooner is an experienced flow cytometrist and will devote 80% time to this Core. A To Be Named Technician will be able to provide backup and cross coverage for the Core. We have decided to have an accomplished full time technician solely dedicated to this COBRE, since this is justified by the complexity and extent of the proposed work. This Core features an InFlux high speed cell sorter from BD Biosciences. This sorter is capable of carrying out stem cell separations, single cell deposition and is housed within a biosafety cabinet. This Core is housed in a dedicated room. The facility is equipped with a new LSRII (BD instruments) with FACS Diva software. Investigators will work with the core and prepare their cell populations for sorting or analysis. The Core will routinely separate Lineage negative Rhodamine lo Hoechst lo (LRH), LT-HSC, ST-HSC, CLP, CMP, LSK, SLAM, and Lineage negative Sca+ stem/progenitor populations. The core will also sort and characterize microvesicles and analyze populations for lineage profiles. The core will serve all projects in this COBRE carrying out both separations and analysis work. The Core also remains dedicated to the development of new separation techniques and the enhancement of known stem cell and microvesicle populations.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR025179-03
Application #
8360131
Study Section
Special Emphasis Panel (ZRR1-RI-2 (01))
Project Start
2011-07-01
Project End
2012-06-30
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
3
Fiscal Year
2011
Total Cost
$253,581
Indirect Cost
Name
Rhode Island Hospital
Department
Type
DUNS #
075710996
City
Providence
State
RI
Country
United States
Zip Code
02903
Li, Ming; Tucker, Lynne D; Asara, John M et al. (2016) Stem-loop binding protein is a multifaceted cellular regulator of HIV-1 replication. J Clin Invest 126:3117-29
Yang, Wentian; Wang, Jianguo; Moore, Douglas C et al. (2013) Ptpn11 deletion in a novel progenitor causes metachondromatosis by inducing hedgehog signalling. Nature 499:491-5
Tang, Xiaoli; Wen, Sicheng; Zheng, Dong et al. (2013) Acetylation of drosha on the N-terminus inhibits its degradation by ubiquitination. PLoS One 8:e72503
Li, Ming; Aliotta, Jason M; Asara, John M et al. (2012) Quantitative proteomic analysis of exosomes from HIV-1-infected lymphocytic cells. Proteomics 12:2203-11
Liu, Liansheng; Papa, Elaine F; Dooner, Mark S et al. (2012) Homing and long-term engraftment of long- and short-term renewal hematopoietic stem cells. PLoS One 7:e31300
Aliotta, Jason M; Lee, David; Puente, Napoleon et al. (2012) Progenitor/stem cell fate determination: interactive dynamics of cell cycle and microvesicles. Stem Cells Dev 21:1627-38
Ellisor, Debra; Rieser, Caroline; Voelcker, Bettina et al. (2012) Genetic dissection of midbrain dopamine neuron development in vivo. Dev Biol 372:249-62
Chan, Gordon; Cheung, Laurene S; Yang, Wentian et al. (2011) Essential role for Ptpn11 in survival of hematopoietic stem and progenitor cells. Blood 117:4253-61
Del Tatto, Michael; Ng, Thomas; Aliotta, Jason M et al. (2011) Marrow cell genetic phenotype change induced by human lung cancer cells. Exp Hematol 39:1072-80
Li, Ming; Patton, Dorothy L; Cosgrove-Sweeney, Yvonne et al. (2011) Incorporation of the HIV-1 microbicide cyanovirin-N in a food product. J Acquir Immune Defic Syndr 58:379-84

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