This application is for renewal of the Nathan Shock Center of Excellence in the Basic Biology of Aging at the University of Washington and affiliated institutions. This Center has over the past 15 years provided resources in support of our large community of investigators that are funded to study basic biological mechanisms of aging. We continue to build upon a scientific theme that became established in the past funding cycle, in which comparative gerontologic study informed us of conserved genetic pathways of longevity determination (the TOR pathway is an example of one such). The three proposed Research Resources Core components are well suited to assist in such efforts: 1) Transgenic Animal Model Development;2) Functional Assessment;3) Functional Genomics. The Transgenic Animal Model Development Resource Core continues to pursue a focus on inducible gene expression and knockout models, so that organ and age-specific effects of genetic alterations can be assessed. The Functional Assessment Core builds upon technical resources that have been gathered during the past funding period, such that we can now provide investigators with a broad and informative range of physiologic and cellular assays, including those that are most relevant to assessment of the healthspan of mouse models. The Functional Genomics Core provides expert assistance with state of the art technologies for transcriptomics and proteomics. Consistent with our theme, it includes a focus on translational state, protein turnover and post-translational modification. The Research Development Core will continue to support pilot study projects in the basic biology of aging. The Program Enrichment Core supports administrative management, an external advisory panel, a program of courses and seminars and data sharing and dissemination.
Continuation of this Center's support will further the utilization of state-of-the-art research resources to provide the strongest environment for the conduct of research on aging biology at institutions in our geographical neighborhood. It will 1) enhance the quality of research in the basic biology of aging, 2) facilitate the planning and coordination of aging biology research activities, 3) provide support and an enriched environment for investigators new to aging biology and 4) contribute to our being able to provide resources to Biology of Aging investigators across the nation.
|Banton, Sophia A; Soltow, Quinlyn A; Liu, Ken H et al. (2016) Plasma Metabolomics of Common Marmosets (Callithrix jacchus) to Evaluate Diet and Feeding Husbandry. J Am Assoc Lab Anim Sci 55:137-46|
|Masel, Joanna; Promislow, Daniel E L (2016) Answering evolutionary questions: A guide for mechanistic biologists. Bioessays 38:704-11|
|Treuting, P M; Snyder, J M; Ikeno, Y et al. (2016) The Vital Role of Pathology in Improving Reproducibility and Translational Relevance of Aging Studies in Rodents. Vet Pathol 53:244-9|
|Kaeberlein, Matt; Creevy, Kate E; Promislow, Daniel E L (2016) The dog aging project: translational geroscience in companion animals. Mamm Genome 27:279-88|
|Hoffman, Jessica M; Tran, ViLinh; Wachtman, Lynn M et al. (2016) A longitudinal analysis of the effects of age on the blood plasma metabolome in the common marmoset, Callithrix jacchus. Exp Gerontol 76:17-24|
|Basisty, Nathan; Dai, Dao-Fu; Gagnidze, Arni et al. (2016) Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy? Aging Cell 15:634-45|
|Sunshine, Anna B; Ong, Giang T; Nickerson, Daniel P et al. (2016) Aneuploidy shortens replicative lifespan in Saccharomyces cerevisiae. Aging Cell 15:317-24|
|Ma, Jiao; Diedrich, Jolene K; Jungreis, Irwin et al. (2016) Improved Identification and Analysis of Small Open Reading Frame Encoded Polypeptides. Anal Chem 88:3967-75|
|Snyder, J M; Ward, J M; Treuting, P M (2016) Cause-of-Death Analysis in Rodent Aging Studies. Vet Pathol 53:233-43|
|Kruse, Shane E; Karunadharma, Pabalu P; Basisty, Nathan et al. (2016) Age modifies respiratory complex I and protein homeostasis in a muscle type-specific manner. Aging Cell 15:89-99|
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