The goal of the Oxidative Damage and Mitochondrial Function Core is two-fold. First, we will continue to provide measurement of oxidative damage to lipid, DNA and protein in cells and tissues using the latest and most sensitive methods and technologic approaches. The second and new function of the Core is to provide several key assays of mitochondrial function including measures of reactive oxygen species generation, respiration and ATP production in isolated mitochondria and in cultured cells. Compromised mitochondrial function and accumulation of oxidative damage to cell components with age have been proposed as primary factors underlying age-associated alterations in physiologic function and pathology. A number of recent studies have suggested that the relation between mitochondrial dysfunction, oxidative damage and aging may not be as straightforward as originally proposed by Hamian more than 50 years ago. However, measures of oxidative damage and mitochondrial function continue to be important components of many studies on the underlying mechanisms of aging and age-related disease, and it is critical that these measures are done with the highest possible sensitivity and accuracy. The measurements offered by the Core require costly equipment and technologic expertise that prevent these types of analyses as routine measures in individual laboratories.
The Specific Aims of the Oxidative Damage and Mitochondrial Function Core are as follows: 1. To provide sensitive and accurate measurements of oxidative damage to lipids, DNA and protein through analysis of F2-isoprostanes/isofurans (lipid oxidation), 8-oxo-2-deoxyguanosine (oxo8dG) (DNA oxidation), and oxidative modifications to proteins (carbonyls, disulfide content and alterations in protein hydrophobicity). 2. To provide high quality sensitive measurement of mitochondrial function in isolated mitochondria and cell culture using state-of-the-art techniques. 3. To provide education and consultation regarding methodology for assessing oxidative damage and mitochondrial function.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZAG1-ZIJ-2)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Texas Health Science Center San Antonio
San Antonio
United States
Zip Code
Sataranatarajan, Kavithalakshmi; Ikeno, Yuji; Bokov, Alex et al. (2016) Rapamycin Increases Mortality in db/db Mice, a Mouse Model of Type 2 Diabetes. J Gerontol A Biol Sci Med Sci 71:850-7
Strong, Randy; Miller, Richard A; Antebi, Adam et al. (2016) Longer lifespan in male mice treated with a weakly estrogenic agonist, an antioxidant, an α-glucosidase inhibitor or a Nrf2-inducer. Aging Cell 15:872-84
Melton, David W; Roberts, Alexander C; Wang, Hanzhou et al. (2016) Absence of CCR2 results in an inflammaging environment in young mice with age-independent impairments in muscle regeneration. J Leukoc Biol 100:1011-1025
Pomilio, Carlos; Pavia, Patricio; Gorojod, Roxana Mayra et al. (2016) Glial alterations from early to late stages in a model of Alzheimer's disease: Evidence of autophagy involvement in Aβ internalization. Hippocampus 26:194-210
Treuting, P M; Snyder, J M; Ikeno, Y et al. (2016) The Vital Role of Pathology in Improving Reproducibility and Translational Relevance of Aging Studies in Rodents. Vet Pathol 53:244-9
Richardson, Arlan; Austad, Steven N; Ikeno, Yuji et al. (2016) Significant life extension by ten percent dietary restriction. Ann N Y Acad Sci 1363:11-7
Richardson, Arlan; Fischer, Kathleen E; Speakman, John R et al. (2016) Measures of Healthspan as Indices of Aging in Mice-A Recommendation. J Gerontol A Biol Sci Med Sci 71:427-30
Salmon, Adam B; Kim, Geumsoo; Liu, Chengyu et al. (2016) Effects of transgenic methionine sulfoxide reductase A (MsrA) expression on lifespan and age-dependent changes in metabolic function in mice. Redox Biol 10:251-256
Wu, Junjie; Sun, Yun; Block, Travis J et al. (2016) Umbilical cord blood-derived non-hematopoietic stem cells retrieved and expanded on bone marrow-derived extracellular matrix display pluripotent characteristics. Stem Cell Res Ther 7:176
Fischer, Kathleen E; Hoffman, Jessica M; Sloane, Lauren B et al. (2016) A cross-sectional study of male and female C57BL/6Nia mice suggests lifespan and healthspan are not necessarily correlated. Aging (Albany NY) 8:2370-2391

Showing the most recent 10 out of 192 publications