Flow cytometry is a unique and powerful technology for analyzing the fluorescent properties of particles and can be readily applied to determining the phenotype and function of cells, as well as utilized for isolating defined cell populations by sorting. In recent years the use of flow cytometric analyses in HIV research has become ever more prominent as newer approaches for dissecting the interactions between HIV and cells of the immune system have been developed. The mission ofthe University of Alabama (UAB) Center for AIDS Research (CFAR) Flow Cytometry Core is thus to maximize the benefits of this technology by providing the necessary instrumentation within a well organized, centralized, facility that is capable of handling potentially infectious material.
The aims of the flow cytometry core are to now build upon our progress-to-date by further enhancing services and training activities so that users are empowered to take full advantage of the sophisticated instrumentation that is available.
We aim to promote innovation by providing a user platform that fosters collaborations and cross-core partnerships that encourage cutting-edge AIDS related research activities. During the last funding period we consolidated the CFAR Flow Cytometry Core with the RDCC Flow Cytometry Core (NIH NOT-RR-10-001). We were able to obtain r $900,000 from various sources to be invested into the flow cytometry infrastructure, which includes the purchase of a new. FACS Aria If cell sorter and several equipment and laser upgrades. During this period, the CFAR Flow Cytometry Core has supported the research activities of 95 Principle Investigators and has contributed to basic, clinical, and translational initiatives in the areas of viral pathogenesis, anti-viral research, cellular immunity, and vaccine design. The core has supported the activities of 78 NIH grants and contributed to more than 150 publications.
The CFAR Flow Cytometry Core is the only facility on the UAB campus that analyses human patient material or other hazardous biological reagents on a routine basis. As such, the presence ofthe core is also vital to a large group of investigators that based on their research are not affiliated with the UAB CFAR and, who are funded by NIH institutes other than the NIAID.
|Rodriguez-Garcia, M; Shen, Z; Barr, F D et al. (2017) Dendritic cells from the human female reproductive tract rapidly capture and respond to HIV. Mucosal Immunol 10:531-544|
|Dubrow, Robert; Qin, Li; Lin, Haiqun et al. (2017) Association of CD4+ T-cell Count, HIV-1 RNA Viral Load, and Antiretroviral Therapy With Kaposi Sarcoma Risk Among HIV-infected Persons in the United States and Canada. J Acquir Immune Defic Syndr 75:382-390|
|Kumar, Ranjit; Yi, Nengjun; Zhi, Degui et al. (2017) Identification of donor microbe species that colonize and persist long term in the recipient after fecal transplant for recurrent Clostridium difficile. NPJ Biofilms Microbiomes 3:12|
|Zhang, Yong; Kwon, Dongjin; Pohl, Kilian M (2017) Computing group cardinality constraint solutions for logistic regression problems. Med Image Anal 35:58-69|
|Willig, Amanda L; Kramer, Philip A; Chacko, Balu K et al. (2017) Monocyte bioenergetic function is associated with body composition in virologically suppressed HIV-infected women. Redox Biol 12:648-656|
|Crane, Heidi M; Nance, Robin M; Merrill, Joseph O et al. (2017) Not all non-drinkers with HIV are equal: demographic and clinical comparisons among current non-drinkers with and without a history of prior alcohol use disorders. AIDS Care 29:177-184|
|Khass, Mohamed; Schelonka, Robert L; Liu, Cun Ren et al. (2017) Alterations in B cell development, CDR-H3 repertoire and dsDNA-binding antibody production among C57BL/6 ?D-iD mice congenic for the lupus susceptibility loci sle1, sle2 or sle3. Autoimmunity 50:42-51|
|Robinson, Tanya O; Zhang, Mingce; Ochsenbauer, Christina et al. (2017) CD4 regulatory T cells augment HIV-1 expression of polarized M1 and M2 monocyte derived macrophages. Virology 504:79-87|
|Saag, Michael S; Westfall, Andrew O; Cole, Stephen R et al. (2017) Brief Report: Factors Associated With the Selection of Initial Antiretroviral Therapy From 2009 to 2012. J Acquir Immune Defic Syndr 74:60-64|
|Pham, Thieng; Perry, Jacob L; Dosey, Timothy L et al. (2017) The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel. Sci Rep 7:43487|
Showing the most recent 10 out of 872 publications