While in vitro models can provide insight into many aspects of viral infection, in vivo models are critical to the understanding of the pathogenesis and treatment of viral diseases. The recent development and use of animal models that display pathology following infection with human immunodeficiency virus type 1 (HIV-1) has significantly expanded our knowledge of the mechanisms associated with viral pathogenesis and provided an impetus towards the development of therapeutic approaches. The development of humanized mouse models has provided novel model systems with which to study the human immune system and the effects of HIV infection on human cells and tissues. These models involve transplantation of various types of human tissues into mice who possess one or more of several types of immune defects. The human tissue can then engraft, grow and develop in a similar fashion as it would in humans, allowing the study of this tissue in a living system. The human tissue used in these studies is capable of a high degree of manipulation and experimentation in the mouse and the human cells, versus the mouse cells, are further susceptible to infection with HIV. This provides a powerful model to examine human bloods cell development, to study the effects of HIV infection on human cells, and ways to protect the human immune system from HIV. The generation of these humanized mice is a highly specialized procedure, due to the requirement for immunodeficient mouse strains, human hematopoietic tissue, infectious material, specialized facilities, and the necessary skill and knowledge to perform experiments in this system. The CFAR supported Humanized Mouse Core is designed to assist AIDS-related investigators at UCLA with their research by providing all of the resources and the environments necessary to support the use of state-of-the art humanized mouse technologies. This facility will support the use of humanized mice for AIDS-related research under both Biosafety Level 2 and Biosafety Level 2+ conditions. This facility will also provide consultation on the use of humanized mouse models, as well as construct various humanized animals for distribution to Core users. Thus, the overall goal of this Core laboratory is to provide the infrastructure, materials, animals, technical expertise and support that will facilitate the use of humanized immunodeficient mice in AIDS-related studies

Public Health Relevance

The overall relevance of the UCLA CFAR Humanized Mouse Core Laboratory is in the ability to provide state-of-the-art resources, expertise, services, and infrastructure involved in AIDS-related humanized mouse-based experiments.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZAI1-UKS-A (J1))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California Los Angeles
Los Angeles
United States
Zip Code
Das, Aritra; Li, Jianjun; Zhong, Fei et al. (2015) Factors associated with HIV and syphilis co-infection among men who have sex with men in seven Chinese cities. Int J STD AIDS 26:145-55
Shin, Sanghyuk S; Modongo, Chawangwa; Ncube, Ronald et al. (2015) Advanced immune suppression is associated with increased prevalence of mixed-strain Mycobacterium tuberculosis infections among persons at high risk for drug-resistant tuberculosis in Botswana. J Infect Dis 211:347-51
Lee, Sung-Jae; Li, Li; Lin, Chunqing et al. (2015) Challenges facing HIV-positive persons who use drugs and their families in Vietnam. AIDS Care 27:283-7
Holloway, Ian W; Padilla, Mark B; Willner, Lauren et al. (2015) Effects of minority stress processes on the mental health of Latino men who have sex with men and women: a qualitative study. Arch Sex Behav 44:2087-97
Epeldegui, Marta; Blom, Bianca; Uittenbogaart, Christel H (2015) BST2/Tetherin is constitutively expressed on human thymocytes with the phenotype and function of Treg cells. Eur J Immunol 45:728-37
Preza, Gloria C; Tanner, Karen; Elliott, Julie et al. (2014) Antigen-presenting cell candidates for HIV-1 transmission in human distal colonic mucosa defined by CD207 dendritic cells and CD209 macrophages. AIDS Res Hum Retroviruses 30:241-9
le Roux, Ingrid M; Rotheram-Borus, Mary Jane; Stein, Judith et al. (2014) The impact of paraprofessional home visitors on infants' growth and health at 18 months. Vulnerable Child Youth Stud 9:291-304
Martin, N T; Nakamura, K; Paila, U et al. (2014) Homozygous mutation of MTPAP causes cellular radiosensitivity and persistent DNA double-strand breaks. Cell Death Dis 5:e1130
Cui, Yijun; Ostlund, Sean B; James, Alex S et al. (2014) Targeted expression of ?-opioid receptors in a subset of striatal direct-pathway neurons restores opiate reward. Nat Neurosci 17:254-61
Remenyi, Roland; Qi, Hangfei; Su, Sheng-Yao et al. (2014) A comprehensive functional map of the hepatitis C virus genome provides a resource for probing viral proteins. MBio 5:e01469-14

Showing the most recent 10 out of 498 publications