Core D - The Flow Cytometry Research Core provides a centralized resource of technical expertise and major instrumentation to support and enhance the experimental design and execution of basic and translational research in HIV pathogenesis and treatment that require use of flow cytometric phenotypic analysis, cell sorting, or evaluation of specific immune cell functions. A prime goal is to provide the scientific environment and the material resources to enable junior investigators to apply flow cytometry technology to their projects and to encourage established investigators to initiate innovative pilot studies. Core personnel include: the Director, Celsa Spina, PhD, D(ABMLI);a lab manager/senior technologist, with >10 yrs. experience using a broad range of cytometry instruments (T. Rambaldo);two technical specialists, each with >20 yrs. experience (M. O'Keefe, N. Sekiya);and a junior technician, with 4 yrs. experience (D. Sirypangno). Major instrumentation includes: three cell analyzers (2-laser FACS Canto I, 3-laser FACS Canto II, 2-laser iCyte LSC) and two high-speed cell sorters (3-laser FACS Aria II, 4-laser MoFlo XDP), one of which is housed within a BSL-2/3 biocontainment facility. The spectrum of HIV/AIDS research, supported by the Core, spans basic work on viral regulation and mechanisms of pathogenesis (AI038201, AI081668, AI095623, AI096113, DK035108, GM032373) to preclinical development of drugs, vaccines, and gene therapies (AI033292, AI055332, AI071803, AI076558, AI079031) to clinical studies of viral transmission (AI074621), viral latency (AI080193, AI096113), neurobiology (DA026306, MH083552, MH097520), and immune and viral responses to experimental treatments (AI064086, AI087164, MH085610). To support and promote research in each of these areas: expert consultation on experimental design and data interpretation is provided through the Core Director and technical support staff;state-of-the-art instrumentation is selected for complementary functions;equipment use is accessible through dedicated technician operators;all services are provided through an established recharge mechanism;and data analysis is customized to the specific objectives of individual research projects. The Core works with investigators to develop and apply new methods that are needed to attain the research goals of funded projects, and trains junior investigators to optimally and correctly use flow cytometry approaches to address their research hypotheses. In addition, the Core fills a special need of the San Diego HIV research community by providing a unique biocontainment facility that performs live cell sorting of HIV-infected and other biohazardous samples.

Public Health Relevance

The Flow Cytometry Research Core provides services and technology that are crucial to the conduct of a broad range of HIV research, including the development of new and better treatments. The Core supports HIV research efforts by providing centralized access to specialized major instrumentation and highly trained, experienced technical personnel that would otherwise not be available to individual investigators.

Agency
National Institute of Health (NIH)
Type
Center Core Grants (P30)
Project #
5P30AI036214-20
Application #
8648957
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
Budget End
Support Year
20
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Chin, Bum Sik; Chaillon, Antoine; Mehta, Sanjay R et al. (2016) Molecular epidemiology identifies HIV transmission networks associated with younger age and heterosexual exposure among Korean individuals. J Med Virol 88:1832-5
Choi, Jun Yong; Chaillon, Antoine; Oh, Jin Ok et al. (2016) HIV migration between blood plasma and cellular subsets before and after HIV therapy. J Med Virol 88:606-13
Gianella, Sara; Kosakovsky Pond, Sergei L; Oliveira, Michelli F et al. (2016) Compartmentalized HIV rebound in the central nervous system after interruption of antiretroviral therapy. Virus Evol 2:vew020
Seifert, Marva; Georghiou, Sophia B; Catanzaro, Donald et al. (2016) MTBDRplus and MTBDRsl Assays: Absence of Wild-Type Probe Hybridization and Implications for Detection of Drug-Resistant Tuberculosis. J Clin Microbiol 54:912-8
Karris, Maile Y; Umlauf, Anya; Vaida, Florin et al. (2016) A randomized controlled clinical trial on the impact of CCR5 blockade with maraviroc in early infection on T-cell dynamics. Medicine (Baltimore) 95:e5315
Hoenigl, Martin; Chaillon, Antoine; Moore, David J et al. (2016) Clear Links Between Starting Methamphetamine and Increasing Sexual Risk Behavior: A Cohort Study Among Men Who Have Sex With Men. J Acquir Immune Defic Syndr 71:551-7
Cáceres, Carlos F; Borquez, Annick; Klausner, Jeffrey D et al. (2016) Implementation of pre-exposure prophylaxis for human immunodeficiency virus infection: progress and emerging issues in research and policy. J Int AIDS Soc 19:21108
Smith, Laramie R; Patterson, Thomas L; Magis-Rodriguez, Carlos et al. (2016) Engagement in the HIV Care Continuum among Key Populations in Tijuana, Mexico. AIDS Behav 20:1017-25
Freeman, Michael L; Lederman, Michael M; Gianella, Sara (2016) Partners in Crime: The Role of CMV in Immune Dysregulation and Clinical Outcome During HIV Infection. Curr HIV/AIDS Rep 13:10-9
Toth, Mate; Flandreau, Elizabeth I; Deslauriers, Jessica et al. (2016) Overexpression of Forebrain CRH During Early Life Increases Trauma Susceptibility in Adulthood. Neuropsychopharmacology 41:1681-90

Showing the most recent 10 out of 754 publications