Flow cytometry is one of the most widely used and indispensable techniques for the analysis and isolation of specific cell populations. The CFAR Flow Cytometry Core was established in 2003 with initial seed funds from the CFAR grant to provide CFAR investigators with improved access to advanced flow cytometry equipment and techniques. The CFAR Core is unique among the flow cytometry resources at Einstein in providing biohazard containment for carrying out analysis and sorting of samples requiring Biosafety Level-2 or higher precautions, and is thus a major resource for work involving HIV and a variety of AIDS-related opportunistic pathogens. The CFAR Flow Cytometry Core provides CFAR investigators with ready access to a variety of advanced instruments for analytical flow cytometry and cell sorting, and extensive technical expertise and assistance for performance of established flow cytometry techniques and implementation of new technologies in this area. In the four years since its inception, the Core has grown substantially with regard to space, equipment and personnel. This reflects the important role that this core facility has played in CFAR related research projects, and also the considerable institutional support that underscores the value added features of the Core. The activities of the Core are organized according to the following four specific aims:
Aim 1. To maintain the necessary research infrastructure needed for flow cytometry studies, with appropriate biosafety containment safeguards.
Aim 2. To maintain cutting edge capabilities by regularly updating existing flow cytometry resources and introducing new equipment and techniques in a timely manner.
Aim 3. To provide convenient and cost-effective access to equipment and technical expertise for the performance and interpretation of flow cytometry.
Aim 4. To provide education and training to assist CFAR investigators in the implementation of new and established flow cytometry techniques.

Public Health Relevance

Flow cytometry allows researchers to study many properties of cells or micro-organisms and rapidly gather information that is crucial to understanding disease processes in animal models and human subjects. The CFAR Flow Cytometry Core supports instruments and personnel that are required for this type of essential research, and organizes these resources in a centralized facilty that provides access for a large number of investigators working on a wide range of problems relevant to HIV infection and AIDS.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
3P30AI051519-10S1
Application #
8628350
Study Section
Special Emphasis Panel (ZAI1-EC-A)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
10
Fiscal Year
2013
Total Cost
$53,046
Indirect Cost
$18,829
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Reimers, Laura L; Mehta, Supriya D; Massad, L Stewart et al. (2016) The Cervicovaginal Microbiota and Its Associations With Human Papillomavirus Detection in HIV-Infected and HIV-Uninfected Women. J Infect Dis 214:1361-1369
Keller, Marla J; McGinn, Aileen P; Lo, Yungtai et al. (2016) Longitudinal Assessment of Systemic and Genital Tract Inflammatory Markers and Endogenous Genital Tract E. coli Inhibitory Activity in HIV-Infected and Uninfected Women. Am J Reprod Immunol 75:631-42
Hanna, David B; Guo, Mengye; Bůžková, Petra et al. (2016) HIV Infection and Carotid Artery Intima-media Thickness: Pooled Analyses Across 5 Cohorts of the NHLBI HIV-CVD Collaborative. Clin Infect Dis 63:249-56
Starrels, Joanna L; Peyser, Deena; Haughton, Lorlette et al. (2016) When human immunodeficiency virus (HIV) treatment goals conflict with guideline-based opioid prescribing: A qualitative study of HIV treatment providers. Subst Abus 37:148-53
Glenn, Matthew C; Sohler, Nancy L; Starrels, Joanna L et al. (2016) Characteristics of methadone maintenance treatment patients prescribed opioid analgesics. Subst Abus 37:387-391
Eugenin, Eliseo A; Berman, Joan W (2016) Improved Methods to Detect Low Levels of HIV Using Antibody-Based Technologies. Methods Mol Biol 1354:265-79
Buckley, Niall; Huber, Ashley; Lo, Yungtai et al. (2016) Association of High-Risk Human Papillomavirus with Genital Tract Mucosal Immune Factors in HIV-Infected Women. Am J Reprod Immunol 75:146-54
Jou, Erin; Gligich, Oleg; Chan, Alvita C Y et al. (2016) Viral co-infections and paraproteins in HIV: effect on development of hematological malignancies. Ann Hematol 95:575-80
La Porte, Annalena; Cano, Jennifer; Wu, Xuhong et al. (2016) An Essential Role of INI1/hSNF5 Chromatin Remodeling Protein in HIV-1 Posttranscriptional Events and Gag/Gag-Pol Stability. J Virol 90:9889-9904
Felsen, Uriel R; Cunningham, Chinazo O; Zingman, Barry S (2016) Increased HIV testing among hospitalized patients who declined testing in the emergency department. AIDS Care 28:591-7

Showing the most recent 10 out of 497 publications