Flow cytometry is one of the most widely used and indispensable techniques for the analysis and isolation of specific cell populations. The CFAR Flow Cytometry Core was established in 2003 with initial seed funds from the CFAR grant to provide CFAR investigators with improved access to advanced flow cytometry equipment and techniques. The CFAR Core is unique among the flow cytometry resources at Einstein in providing biohazard containment for carrying out analysis and sorting of samples requiring Biosafety Level-2 or higher precautions, and is thus a major resource for work involving HIV and a variety of AIDS-related opportunistic pathogens. The CFAR Flow Cytometry Core provides CFAR investigators with ready access to a variety of advanced instruments for analytical flow cytometry and cell sorting, and extensive technical expertise and assistance for performance of established flow cytometry techniques and implementation of new technologies in this area. In the four years since its inception, the Core has grown substantially with regard to space, equipment and personnel. This reflects the important role that this core facility has played in CFAR related research projects, and also the considerable institutional support that underscores the value added features of the Core. The activities of the Core are organized according to the following four specific aims:
Aim 1. To maintain the necessary research infrastructure needed for flow cytometry studies, with appropriate biosafety containment safeguards.
Aim 2. To maintain cutting edge capabilities by regularly updating existing flow cytometry resources and introducing new equipment and techniques in a timely manner.
Aim 3. To provide convenient and cost-effective access to equipment and technical expertise for the performance and interpretation of flow cytometry.
Aim 4. To provide education and training to assist CFAR investigators in the implementation of new and established flow cytometry techniques.
Flow cytometry allows researchers to study many properties of cells or micro-organisms and rapidly gather information that is crucial to understanding disease processes in animal models and human subjects. The CFAR Flow Cytometry Core supports instruments and personnel that are required for this type of essential research, and organizes these resources in a centralized facilty that provides access for a large number of investigators working on a wide range of problems relevant to HIV infection and AIDS.
|Rao, Vasudev R; Neogi, Ujjwal; Eugenin, Eliseo et al. (2014) The gp120 protein is a second determinant of decreased neurovirulence of Indian HIV-1C isolates compared to southern African HIV-1C isolates. PLoS One 9:e107074|
|Singh, M; Quispe-Tintaya, W; Chandra, D et al. (2014) Direct incorporation of the NKT-cell activator ?-galactosylceramide into a recombinant Listeria monocytogenes improves breast cancer vaccine efficacy. Br J Cancer 111:1945-54|
|Jelen, Mateja M; Chen, Zigui; Kocjan, Boštjan J et al. (2014) Global genomic diversity of human papillomavirus 6 based on 724 isolates and 190 complete genome sequences. J Virol 88:7307-16|
|Tarassishin, Leonid; Suh, Hyeon-Sook; Lee, Sunhee C (2014) LPS and IL-1 differentially activate mouse and human astrocytes: role of CD14. Glia 62:999-1013|
|Strickler, Howard D; Martinson, Jeffrey; Desai, Seema et al. (2014) The relation of plasmacytoid dendritic cells (pDCs) and regulatory T-cells (Tregs) with HPV persistence in HIV-infected and HIV-uninfected women. Viral Immunol 27:20-5|
|Tarassishin, Leonid; Casper, Diana; Lee, Sunhee C (2014) Aberrant expression of interleukin-1? and inflammasome activation in human malignant gliomas. PLoS One 9:e103432|
|Prados-Rosales, Rafael; Carreño, Leandro J; Batista-Gonzalez, Ana et al. (2014) Mycobacterial membrane vesicles administered systemically in mice induce a protective immune response to surface compartments of Mycobacterium tuberculosis. MBio 5:e01921-14|
|Stec, Jozef; Vilchèze, Catherine; Lun, Shichun et al. (2014) Biological evaluation of potent triclosan-derived inhibitors of the enoyl-acyl carrier protein reductase InhA in drug-sensitive and drug-resistant strains of Mycobacterium tuberculosis. ChemMedChem 9:2528-37|
|Benning, Lorie; Golub, Elizabeth T; Anastos, Kathryn et al. (2014) Comparison of lower genital tract microbiota in HIV-infected and uninfected women from Rwanda and the US. PLoS One 9:e96844|
|Shuter, Jonathan; Moadel, Alyson B; Kim, Ryung S et al. (2014) Self-efficacy to quit in HIV-infected smokers. Nicotine Tob Res 16:1527-31|
Showing the most recent 10 out of 381 publications