The goal of the IMMUNOLOGY AND PATHOLOGY CORE is to provide a centralized facility for performing a range of assays and services that facilitate the functional and anatomical analysis of cells and tissues by CFAR investigators. This facility provides the biosafety containment that is necessary for these analyses. Services provided by this core include RNA and protein analyses for cytokines, chemokines and growth factors, as well as tissue fixation, slide preparation and histopathology using microscopic examination, immunohistochemical and in situ hybridization techniques. We will soon offer additional cutting edge technology by providing use of an inverted fluorescence microscope to analyze HIV infected living cells and unfixed tissues. This core provides a key service, as an understanding of the mechanisms that mediate HIV and opportunistic infection and tissue responses requires an analysis of the factors being elaborated by and the phenotypes of infected cells as well as the response to infection by uninfected resident cells. Therefore, access to these services greatly supports the diverse research efforts of investigators at Einstein and Montefiore aimed at understanding the pathogenesis of infection with HIV-1 and AIDS-associated pathogens.
Aim 1. To provide investigators with the research infrastructure that is necessary to analyze the immune responses stimulated by HIV and AIDS-associated pathogens by measuring cytokine, chemokine and other soluble mediator, RNA, and protein levels in the appropriate biosafety containment.
Aim 2. To provide investigators with a facility using appropriate biohazard containment that is capable of examining the effects of infection with HIV and AIDS-associated pathogens on tissues by characterizing morphological, molecular and phenotypical changes using histological, immunohistochemical, in situ hybridization analyses and live cell imaging.
Aim 3. To train CFAR investigators in the performance of techniques that are extant in the Core and to assist investigators in the development of new immunological assays and pathological techniques that are required for the accomplishment of their research projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
3P30AI051519-10S1
Application #
8628354
Study Section
Special Emphasis Panel (ZAI1-EC-A)
Project Start
Project End
Budget Start
2013-05-01
Budget End
2014-04-30
Support Year
10
Fiscal Year
2013
Total Cost
$200,035
Indirect Cost
$71,003
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Reimers, Laura L; Mehta, Supriya D; Massad, L Stewart et al. (2016) The Cervicovaginal Microbiota and Its Associations With Human Papillomavirus Detection in HIV-Infected and HIV-Uninfected Women. J Infect Dis 214:1361-1369
Keller, Marla J; McGinn, Aileen P; Lo, Yungtai et al. (2016) Longitudinal Assessment of Systemic and Genital Tract Inflammatory Markers and Endogenous Genital Tract E. coli Inhibitory Activity in HIV-Infected and Uninfected Women. Am J Reprod Immunol 75:631-42
Hanna, David B; Guo, Mengye; Bůžková, Petra et al. (2016) HIV Infection and Carotid Artery Intima-media Thickness: Pooled Analyses Across 5 Cohorts of the NHLBI HIV-CVD Collaborative. Clin Infect Dis 63:249-56
Starrels, Joanna L; Peyser, Deena; Haughton, Lorlette et al. (2016) When human immunodeficiency virus (HIV) treatment goals conflict with guideline-based opioid prescribing: A qualitative study of HIV treatment providers. Subst Abus 37:148-53
Glenn, Matthew C; Sohler, Nancy L; Starrels, Joanna L et al. (2016) Characteristics of methadone maintenance treatment patients prescribed opioid analgesics. Subst Abus 37:387-391
Eugenin, Eliseo A; Berman, Joan W (2016) Improved Methods to Detect Low Levels of HIV Using Antibody-Based Technologies. Methods Mol Biol 1354:265-79
Buckley, Niall; Huber, Ashley; Lo, Yungtai et al. (2016) Association of High-Risk Human Papillomavirus with Genital Tract Mucosal Immune Factors in HIV-Infected Women. Am J Reprod Immunol 75:146-54
Jou, Erin; Gligich, Oleg; Chan, Alvita C Y et al. (2016) Viral co-infections and paraproteins in HIV: effect on development of hematological malignancies. Ann Hematol 95:575-80
La Porte, Annalena; Cano, Jennifer; Wu, Xuhong et al. (2016) An Essential Role of INI1/hSNF5 Chromatin Remodeling Protein in HIV-1 Posttranscriptional Events and Gag/Gag-Pol Stability. J Virol 90:9889-9904
Felsen, Uriel R; Cunningham, Chinazo O; Zingman, Barry S (2016) Increased HIV testing among hospitalized patients who declined testing in the emergency department. AIDS Care 28:591-7

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