Due to outstanding progress and important developments in the field of chimeric mouse models reconstituted with human immune systems and cells, Core H, the Small Animal Biocontainment (ABC) Core expanded its scope and now includes two sites. Site A is located at the Massachusetts General Hospital (MGH), and Site B is the existing BL-3 ABC Facility at the Dana-Farber Cancer Institute (DFCI). Site A will provide the following services: 1. Generation of humanized BLT mice (NOD-SCID-Hu mice transplanted wit human fetal thymus, liver and hematopoietic stems cells (HSC)) for CFAR investigators to use for experiments, to be performed at the MGH or DFCI Sites of this core, or at the site of the collaborating investigator, following transfer of the BLT mice to his/her animal facility. 2. Bleeding and flow cytometry of peripheral blood leukocytes of the BLT mice to characterize their human immune reconstitution prior to use by collaborating investigators. 3. For collaborating investigators choosing to perform their experiments at the MGH site, support in the handling and analysis of HIV-infected mice. 4. For collaborating investigators choosing to perform experiments in their own animal facilities, training in the safe handling of HIV-infected mice. 5. Further characterization of the human virus-specific cellular and humoral immune responses generated in HIV-infected BLT mice, to expand the areas of investigation this model can support. Site B will provide the following services: 1. The use of an animal facility in which the spread of contagious agents is prevented between animals. 2. Technical support in handling animals potentially infectious to humans or animals. 3. Access to technical support for performing HIV-1 inoculations into chimeric BLT or NOG (i.e., NOD/SCID.IL-2RYNull) mice reconstituted with CD34+ human HSC, which will be housed to evaluate candidate antiviral agents for efficacy and toxicity in these novel small animal models. A special plus of Site B is the ability to permit work with Mycobacterium tuberculosis (MTb) or HIV-1/MTb co-infections. 4. The use of safe working conditions. 5. Training for safe handling of potential human pathogens in small animal models.

Public Health Relevance

Core H will allow CFAR investigators to study HIV immunopathogenesis and host-virus dynamics in small animal models, which can also support coinfection with HIV-1 and Mycobacterium tuberculosis, two pathogens that threaten public health worldwide.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
5P30AI060354-10
Application #
8523746
Study Section
Special Emphasis Panel (ZAI1-SV-A)
Project Start
Project End
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
10
Fiscal Year
2013
Total Cost
$302,698
Indirect Cost
$50,598
Name
Harvard University
Department
Type
DUNS #
082359691
City
Cambridge
State
MA
Country
United States
Zip Code
02138
Ramirez-Avila, Lynn; Regan, Susan; Chetty, Senica et al. (2015) HIV testing rates, prevalence, and knowledge among outpatients in Durban, South Africa: Time trends over four years. Int J STD AIDS 26:704-9
Psaros, Christina; Barinas, Jennifer; Robbins, Gregory K et al. (2015) Reflections on living with HIV over time: exploring the perspective of HIV-infected women over 50. Aging Ment Health 19:121-8
Drain, Paul K; Losina, Elena; Coleman, Sharon M et al. (2015) Value of urine lipoarabinomannan grade and second test for optimizing clinic-based screening for HIV-associated pulmonary tuberculosis. J Acquir Immune Defic Syndr 68:274-80
Chan, Brian T; Weiser, Sheri D; Boum, Yap et al. (2015) Persistent HIV-related stigma in rural Uganda during a period of increasing HIV incidence despite treatment expansion. AIDS 29:83-90
Blashill, Aaron J; Goshe, Brett M; Robbins, Gregory K et al. (2014) Body image disturbance and health behaviors among sexual minority men living with HIV. Health Psychol 33:677-80
Rothchild, Alissa C; Jayaraman, Pushpa; Nunes-Alves, Cláudio et al. (2014) iNKT cell production of GM-CSF controls Mycobacterium tuberculosis. PLoS Pathog 10:e1003805
Powis, Kathleen; Lockman, Shahin; Smeaton, Laura et al. (2014) Vitamin D insufficiency in HIV-infected pregnant women receiving antiretroviral therapy is not associated with morbidity, mortality or growth impairment in their uninfected infants in Botswana. Pediatr Infect Dis J 33:1141-7
Li, Hualin; Stephenson, Kathryn E; Kang, Zi Han et al. (2014) Common features of mucosal and peripheral antibody responses elicited by candidate HIV-1 vaccines in rhesus monkeys. J Virol 88:13510-5
Sixsmith, Jaimie D; Panas, Michael W; Lee, Sunhee et al. (2014) Recombinant Mycobacterium bovis bacillus Calmette-Guérin vectors prime for strong cellular responses to simian immunodeficiency virus gag in rhesus macaques. Clin Vaccine Immunol 21:1385-95
Weinstein, Edward A; Ordonez, Alvaro A; DeMarco, Vincent P et al. (2014) Imaging Enterobacteriaceae infection in vivo with 18F-fluorodeoxysorbitol positron emission tomography. Sci Transl Med 6:259ra146

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