CoreC The proposed Duke CFAR Flow Cytometry Core Facility will a) provide state-of-the-art, multi-color, BSL-3 fluorescence activated cell sorting support for basic research or translational investigations conducted by Duke CFAR members, including sorting HIV-1 infected cells, b) provide state-of-the-art, multi-color, BSL-2* flow cytometric analytical support for basic, translational, and clinical research investigations conducted by Duke CFAR members, and c) enhance the scientific approach used by Duke CFAR Investigators by educating the Core User base in more effective ways of utilizing the state-of-the-art tools offered by the Duke CFAR Flow Cytometry Core Facility, as well as to provide programmatic advancement for Managers and Operators of the CFAR Flow Cytometry Core Facility. The BSL-3 Sorting Component Laboratory will house the only flow cytometer at Duke University Medical Center capable of sorting live, unfixed human or animal derived cells, infected with HIV-1 or equivalent pathogens, and will prove an invaluable asset to the overall scope of the proposed Duke CFAR Flow Cytometry Core Facility. The Duke CFAR Flow Cytometry Core will be the only flow cytometry facility at Duke capable of providing polychromatic and flow informatic support for HIV and HIV-related translational research at Duke. The proposed Duke CFAR Flow Cytometry Core Facility will be comprised of two Component Laboratories, both housed in biocontainment laboratories within the SORF &Global Health Buildings, respectively. The Component Laboratories will be closely linked physically, administratively, and scientifically, in order to form a highly interactive and unified Duke CFAR Flow Cytometry Core Facility. The BSL-2* Analytical Component Laboratory will provide a broad range of polychromatic flow cytometry applications to CFAR investigators and, together with the BSL-3 Sorting Component Laboratory, comprise the only flow cytometry research facility on campus that routinely processes, sorts, and analyzes HIV-1 infected specimens.

Public Health Relevance

This Core makes state of the art immune monitoring technologies available to Duke scientists at the forefront of AIDS research. These tools will greatly facilitate rapid progress in the development of novel AIDS therapies and preventative AIDS vaccines.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Duke University
United States
Zip Code
Mkhize, Nonhlanhla N; Durgiah, Raveshni; Ashley, Vicki et al. (2016) Broadly neutralizing antibody specificities detected in the genital tract of HIV-1 infected women. AIDS 30:1005-14
Brochhausen, Mathias; Zheng, Jie; Birtwell, David et al. (2016) OBIB-a novel ontology for biobanking. J Biomed Semantics 7:23
Kidd, Marie J; Jackson, Katherine J L; Boyd, Scott D et al. (2016) DJ Pairing during VDJ Recombination Shows Positional Biases That Vary among Individuals with Differing IGHD Locus Immunogenotypes. J Immunol 196:1158-64
Ramaiya, Megan K; Sullivan, Kristen A; O' Donnell, Karen et al. (2016) A Qualitative Exploration of the Mental Health and Psychosocial Contexts of HIV-Positive Adolescents in Tanzania. PLoS One 11:e0165936
Kelly, Matthew S; Crotty, Eric J; Rattan, Mantosh S et al. (2016) Chest Radiographic Findings and Outcomes of Pneumonia Among Children in Botswana. Pediatr Infect Dis J 35:257-62
Healy, Zachary R; Murdoch, David M (2016) OMIP-036: Co-inhibitory receptor (immune checkpoint) expression analysis in human T cell subsets. Cytometry A 89:889-892
Park, Se Eun; Pak, Gi Deok; Aaby, Peter et al. (2016) The Relationship Between Invasive Nontyphoidal Salmonella Disease, Other Bacterial Bloodstream Infections, and Malaria in Sub-Saharan Africa. Clin Infect Dis 62 Suppl 1:S23-31
Pacek, Lauren R; Sweitzer, Maggie M; McClernon, F Joseph (2016) Non-cigarette tobacco and poly-tobacco use among persons living with HIV drawn from a nationally representative sample. Drug Alcohol Depend 162:251-5
Bengtson, Angela M; Pence, Brian W; Gaynes, Bradley N et al. (2016) Improving Depression Among HIV-Infected Adults: Transporting the Effect of a Depression Treatment Intervention to Routine Care. J Acquir Immune Defic Syndr 73:482-488
Abedini-Nassab, Roozbeh; Joh, Daniel Y; Albarghouthi, Faris et al. (2016) Magnetophoretic transistors in a tri-axial magnetic field. Lab Chip 16:4181-4188

Showing the most recent 10 out of 390 publications