The Basic Science Core (BSC;Core D) will enhance basic and laboratory-based HIV/AIDS research by CFAR members, by providing access to cutting-edge instrumentation and technologies, and the necessary support, education and training to enable efficient use of these resources. The services provided by Core D will create added value by addressing resource gaps on campus, and by providing CFAR SWG members with access to enhanced services for chemical biology and drug development. Over the funding period of our D-CFAR, Core D has been highly successful in leveraging new equipment valued at over $2M (a 18:1 ROI), and in helping to create a new strategic partnership between UR and Temple University's Moulder Center for Drug Discovery Research. The Core has also developed a highly proactive, user-oriented, approach that has allowed it to serve almost half (42%) of the total DCFAR membership. Building on this solid foundation, five Aims are proposed.
Aim 1 will provide cutting-edge tools and standardized reagents for virology, immunology, and molecular biology of HIV-1, including recombinant Tat for our CNS Reservoirs/Aging SWG, and a new FPLC resource for our RNA Biology SWG.
Aim 2 will provide expertise and resources for chemical and structural biology, including full-service medicinal chemistry resources and a new pilot program with the Moulder Center, of value to both SWGs.
Aim 3 will provide access to next-generation flow cytometry, including imaging- and mass- cytometry. Finally, Aim 4 will provide training and education to promote the use of new tools, and Aim 5 will integrate Core D services with other Cores and institutional resources - thus ensuring comprehensive attention to investigator needs.

Public Health Relevance

; The Basic Science Core (BSC;Core D) will enhance basic and laboratory-based HIV/AIDS research by CFAR members, by providing access to cutting-edge instrumentation and technologies, and the necessary support, education and training to enable efficient use of these resources.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
5P30AI078498-07
Application #
8712927
Study Section
Special Emphasis Panel (ZAI1-UKS-A)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
7
Fiscal Year
2014
Total Cost
$234,863
Indirect Cost
$81,858
Name
Case Western Reserve University
Department
Type
DUNS #
041294109
City
Rochester
State
NY
Country
United States
Zip Code
14627
McMahon, James M; Pouget, Enrique R; Tortu, Stephanie et al. (2015) Couple-based HIV counseling and testing: a risk reduction intervention for US drug-involved women and their primary male partners. Prev Sci 16:341-51
McMahon, James M; Volpe, Ellen M; Klostermann, Keith et al. (2015) A systematic review of the psychometric properties of the Sexual Relationship Power Scale in HIV/AIDS research. Arch Sex Behav 44:267-94
Bobo, Bryan; Phelan, Dana; Rebhahn, Jonathan et al. (2014) Microbubble array diffusion assay for the detection of cell secreted factors. Lab Chip 14:3640-50
Liu, Zhi-Ping; Wu, Hulin; Zhu, Jian et al. (2014) Systematic identification of transcriptional and post-transcriptional regulations in human respiratory epithelial cells during influenza A virus infection. BMC Bioinformatics 15:336
Adler, David H; Wallace, Melissa; Bennie, Thola et al. (2014) Cervical dysplasia and high-risk human papillomavirus infections among HIV-infected and HIV-uninfected adolescent females in South Africa. Infect Dis Obstet Gynecol 2014:498048
Lewis, Cindi A; Dewhurst, Stephen; McMahon, James M et al. (2014) Theoretical model of critical issues in informed consent in HIV vaccine trials. AIDS Care 26:1452-60
McMahon, James M; Myers, Julie E; Kurth, Ann E et al. (2014) Oral pre-exposure prophylaxis (PrEP) for prevention of HIV in serodiscordant heterosexual couples in the United States: opportunities and challenges. AIDS Patient Care STDS 28:462-74
Piekna-Przybylska, Dorota; Sullivan, Mark A; Sharma, Gaurav et al. (2014) U3 region in the HIV-1 genome adopts a G-quadruplex structure in its RNA and DNA sequence. Biochemistry 53:2581-93
Morse, Diane S; Cerulli, Catherine; Bedell, Precious et al. (2014) Meeting health and psychological needs of women in drug treatment court. J Subst Abuse Treat 46:150-7
Silva, Jharon N; Polesskaya, Oksana; Wei, Helen S et al. (2014) Chronic central nervous system expression of HIV-1 Tat leads to accelerated rarefaction of neocortical capillaries and loss of red blood cell velocity heterogeneity. Microcirculation 21:664-76

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