The SDRC Cell Culture and Molecular Technology Core (CCMTC) provides established, well characterized skin cell lines and primary cultures, as well as services and training in systems biology approaches to the study of skin cells. The core continuously evolves depending on investigator needs and provides state-of-the- art services, training, facilities and expertise. The CCMTC has developed into a major hub of research activity on campus and has an active role in introducing new investigators to work on cutaneous disease. The core is heavily engaged in providing a wide range of cultured skin cells to investigators, and in providing training in cell culture methodology and emerging molecular and systems biology technology. The three major missions of the CCMTC are listed below.
Specific Aim 1 : To provide cost-effective service and training in state-of-the-art cell culture and molecular technology to promote skin-related research. List of activities include the provision of cultured epidermal and dermal cells (keratinocytes, melanocytes, fibroblasts and immunocytes) to SDRC investigators in an efficient, quality-controlled, and cost effective manner. Provision of, access to and training in a wide range of molecular biologic technologies (e.g., siRNA methodology, retroviral immortalization, vector-based gene delivery, etc.).
Specific Aim 2 : To provide SDRC investigators with skin-specific 'Omics and systems biology data analysis, consultation, technologies and software to facilitate a "pipeline" of inter-core translational research and interdisciplinary projects involving skin research. The CCMTC serves as a knowledge base for skin-specific transcriptomics and proteomics and provides expertise with the plan, design and bioinformatics analysis of these emerging technologies.
Specific Aim 3 : To promote career development with a skin-centered emphasis for research residents, fellows and faculty members by helping them to learn state of the art cell culture and molecular techniques as they build a body of preliminary data for research projects. Due to investigator demand, the CCMTC will now offer several new technologies including metabolome analysis from skin cells/tissue, DASL assay for gene expression profiles from paraffin-embedded tissue, and whole genome methylation for epigenetic studies.

Public Health Relevance

The CCMTC is a critical component of the SDRC effort to promote skin-related research at Case. The CCMTC has evolved over time to respond to member needs, innovation, and new expertise. There are many examples of collaborations and interdisciplinary projects that draw on the SDRC services. Trainee education and career development will continue to be a major theme in this SDRC initiative. More than 40 SDRC faculty and many more trainees have utilized the CCMTC since the last funding period.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Center Core Grants (P30)
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Special Emphasis Panel (ZAR1-HL)
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Case Western Reserve University
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Mirmirani, P; Consolo, M; Oyetakin-White, P et al. (2015) Similar response patterns to topical minoxidil foam 5% in frontal and vertex scalp of men with androgenetic alopecia: a microarray analysis. Br J Dermatol 172:1555-61
Hatter, Alyn D; Zhou, Xin; Honda, Kord et al. (2015) Langerhans Cell Hyperplasia From Molluscum Contagiosum. Am J Dermatopathol 37:e93-5
Hamburg-Shields, Emily; DiNuoscio, Gregg J; Mullin, Nathaniel K et al. (2015) Sustained ?-catenin activity in dermal fibroblasts promotes fibrosis by up-regulating expression of extracellular matrix protein-coding genes. J Pathol 235:686-97
Zhou, Hua-Lin; Luo, Guangbin; Wise, Jo Ann et al. (2014) Regulation of alternative splicing by local histone modifications: potential roles for RNA-guided mechanisms. Nucleic Acids Res 42:701-13
Lam, Minh; Dimaano, Matthew L; Oyetakin-White, Patricia et al. (2014) Silicon phthalocyanine 4 phototoxicity in Trichophyton rubrum. Antimicrob Agents Chemother 58:3029-34
Ward, Nicole L; Umetsu, Dale T (2014) A new player on the psoriasis block: IL-17A- and IL-22-producing innate lymphoid cells. J Invest Dermatol 134:2305-7
Feng, Zhimin; Jia, Xun; Adams, Mark D et al. (2014) Epithelial innate immune response to Acinetobacter baumannii challenge. Infect Immun 82:4458-65
Chung, Charlotte Y; Alden, Stephanie L; Funderburg, Nicholas T et al. (2014) Progressive proximal-to-distal reduction in expression of the tight junction complex in colonic epithelium of virally-suppressed HIV+ individuals. PLoS Pathog 10:e1004198
Guan, D; Lim, J H; Peng, L et al. (2014) Deacetylation of the tumor suppressor protein PML regulates hydrogen peroxide-induced cell death. Cell Death Dis 5:e1340
Kambara, Hiroto; Niazi, Farshad; Kostadinova, Lenche et al. (2014) Negative regulation of the interferon response by an interferon-induced long non-coding RNA. Nucleic Acids Res 42:10668-80

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