The Morphology Core (A) is an integrated cellular, histological and pathological laboratory focusing on providing tissue processing and histological expertise, state of the art microscopy and image analyses, whole animal imaging technologies and skin specific pathological interpretation. The Morphology Core has expanded its focus and has added new areas of technology to reflect the shift in projects undertaken by SDRC faculty utilizing the Morphology Core. There has been a very high demand for sophisticated high throughput confocal imaging as well as live cell imaging capabilities, computer-assisted image analysis, preparation of tissue for laser capture microdissection and flow cytometry. As a consequence, the Morphology Core has maintained resources for high-demand services and committed resources to these new demand areas, as reflected in the Specific Aims.
Specific Aim I. Provide cost effective state-of-the-art skin specific histological, microscopic, morphometric and analytic imaging services and expertise to promote skin-related research including (i) embedding, sectioning, and staining facilities, routine histology services for human and murine samples;(ii) immunolocalization methods for morphological studies;(iii) expert microscopy and photo-microscopy with hands-on training in a range of microscopic technologies;(iv) expert pathology consultation of human and mouse tissue, (v) experimental design assistance for experiments requiring morphological analysis, including macro development, statistical planning and analysis;(vi) quantitative image analysis;(vii) confocal laser microscopy;(viii) laser capture microdissection;and (ix) flow cytometry. Included in this application are new resource(s) for SDRC members including (i) spinning disk confocal microscopy, including live cell imaging and high throughput screening technologies;(ii) small animal imaging and (iii) in house flow cytometry.
Specific Aim II. Provide expertise in morphologic techniques that facilitate a """"""""pipeline"""""""" of translational research and interdisciplinary projects involving skin research. The Morphology Core promotes novel bidirectional research that benefits patients with skin disease by supporting projects that lead from bedside to bench and bench to bedside {translational research). Additional goals include disseminating technical information among SDRC members, encouraging resource sharing, providing mentorship, and enhancing collaborations that promote interdisciplinary exchange of ideas and expertise.

Public Health Relevance

The Morphology Core provides expert knowledge for skin-specific tissue analyses, protocol development and morphological and pathological analyses, and provides expertise and training in microscopy and image analyses and evolves and changes to meet the needs of new SDRC investigators and new research directions. The Morphology core is heavily utilized and is integral to the success of the SDRC.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZAR1-HL)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Case Western Reserve University
United States
Zip Code
Larkin, Emily; Hager, Christopher; Chandra, Jyotsna et al. (2017) The Emerging Pathogen Candida auris: Growth Phenotype, Virulence Factors, Activity of Antifungals, and Effect of SCY-078, a Novel Glucan Synthesis Inhibitor, on Growth Morphology and Biofilm Formation. Antimicrob Agents Chemother 61:
Tacastacas, Joselin D; Chan, Derek V; Carlson, Sean et al. (2017) Evaluation of O6-Benzylguanine-Potentiated Topical Carmustine for Mycosis Fungoides: A Phase 1-2 Clinical Trial. JAMA Dermatol 153:413-420
Das, Lopa M; Binko, Amy M; Traylor, Zachary P et al. (2017) Defining the timing of 25(OH)D rescue following nitrogen mustard exposure. Cutan Ocul Toxicol :1-6
Swindell, William R; Sarkar, Mrinal K; Liang, Yun et al. (2017) RNA-seq identifies a diminished differentiation gene signature in primary monolayer keratinocytes grown from lesional and uninvolved psoriatic skin. Sci Rep 7:18045
Mukherjee, Pranab K; Chandra, Jyotsna; Retuerto, Mauricio et al. (2017) Effect of alcohol-based hand rub on hand microbiome and hand skin health in hospitalized adult stem cell transplant patients: a pilot study. J Am Acad Dermatol :
Mullin, Nathaniel K; Mallipeddi, Nikhil V; Hamburg-Shields, Emily et al. (2017) Wnt/?-catenin Signaling Pathway Regulates Specific lncRNAs That Impact Dermal Fibroblasts and Skin Fibrosis. Front Genet 8:183
Arbiser, Jack L; Nowak, Ron; Michaels, Kellie et al. (2017) Evidence for biochemical barrier restoration: Topical solenopsin analogs improve inflammation and acanthosis in the KC-Tie2 mouse model of psoriasis. Sci Rep 7:11198
Tacastacas, Joselin D; Oyetakin-White, Patricia; Soler, David C et al. (2017) Does imiquimod pretreatment optimize 308-nm excimer laser (UVB) therapy in psoriasis patients? Photodermatol Photoimmunol Photomed 33:193-202
Fritz, Yi; Klenotic, Philip A; Swindell, William R et al. (2017) Induction of Alternative Proinflammatory Cytokines Accounts for Sustained Psoriasiform Skin Inflammation in IL-17C+IL-6KO Mice. J Invest Dermatol 137:696-705
Hutnick, Melanie A; Ahsanuddin, Sayeeda; Guan, Linna et al. (2017) PEGylated Dendrimers as Drug Delivery Vehicles for the Photosensitizer Silicon Phthalocyanine Pc 4 for Candidal Infections. Biomacromolecules 18:379-385

Showing the most recent 10 out of 399 publications