Abstract

The SCID Mouse: Skin xenograft core utilizes severe combined immunodeficient (SCID) beige double mutant mice to host living of human skin (and skin-related cells) in the form of split thickness skin grafts or synthetic skin constructs. The three specific aims of the core are (1) to provide members of the YSDRC (both established and junior investigators, including investigators with YSDRC P/F projects) with limited numbers of C.B-17 SCID/beige mice engrafted with healthy adult human skin or skin constructs for use in a variety of skin disease-related studies, including feasibility studies; (2) to train members of the YSDRC to generate such mice once feasibility has been established; and (3) to develop variant models of this technique for new and novel applications, specifically focussing on synthetic skin equivalents constructed from distinct populations of cells, that in some cases may be genteically modified to alter biologic functions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
2P30AR041942-11
Application #
6756347
Study Section
Special Emphasis Panel (ZAR1-AAA-G (J1))
Project Start
2004-04-01
Project End
2009-03-31
Budget Start
2004-04-01
Budget End
2005-03-31
Support Year
11
Fiscal Year
2004
Total Cost
$101,370
Indirect Cost

  Institution

Name
Yale University
Department
Type
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Dainichi, Teruki; Hayden, Matthew S; Park, Sung-Gyoo et al. (2016) PDK1 Is a Regulator of Epidermal Differentiation that Activates and Organizes Asymmetric Cell Division. Cell Rep 15:1615-23
Askenase, Phillip W; Bryniarski, Krzysztof; Paliwal, Vipin et al. (2015) A subset of AID-dependent B-1a cells initiates hypersensitivity and pneumococcal pneumonia resistance. Ann N Y Acad Sci 1362:200-14
Clark, Paul R; Jensen, Todd J; Kluger, Martin S et al. (2011) MEK5 is activated by shear stress, activates ERK5 and induces KLF4 to modulate TNF responses in human dermal microvascular endothelial cells. Microcirculation 18:102-17
Kwong, Bernice Y; Roberts, Scott J; Silberzahn, Tobias et al. (2010) Molecular analysis of tumor-promoting CD8+ T cells in two-stage cutaneous chemical carcinogenesis. J Invest Dermatol 130:1726-36
Radtke, Christine; Vogt, Peter M; Devor, Marshall et al. (2010) Keratinocytes acting on injured afferents induce extreme neuronal hyperexcitability and chronic pain. Pain 148:94-102
Kirkiles-Smith, Nancy C; Harding, Martha J; Shepherd, Benjamin R et al. (2009) Development of a humanized mouse model to study the role of macrophages in allograft injury. Transplantation 87:189-97
Koga, Yasuo; Pelizzola, Mattia; Cheng, Elaine et al. (2009) Genome-wide screen of promoter methylation identifies novel markers in melanoma. Genome Res 19:1462-70
Yates, Kristin E; Korbel, Gregory A; Shtutman, Michael et al. (2008) Repression of the SUMO-specific protease Senp1 induces p53-dependent premature senescence in normal human fibroblasts. Aging Cell 7:609-21
Pelizzola, Mattia; Koga, Yasuo; Urban, Alexander Eckehart et al. (2008) MEDME: an experimental and analytical methodology for the estimation of DNA methylation levels based on microarray derived MeDIP-enrichment. Genome Res 18:1652-9
Shen, Xiaoyan; Berger, Carole L; Tigelaar, Robert et al. (2008) Development of immunogenic tumor-loaded dendritic cells through physical perturbation and apoptotic cell loading. Immunol Invest 37:798-821

Showing the most recent 10 out of 97 publications