The goals of the Columbia University Medical Center Skin Disease Research Center are to understand the developmental biology of the skin, and to understand the pathogenic interactions between immune cells and target cells in the skin in humans. These goals require sophisticated processing and analysis of primary human tissues and blood. Since these materials are precious and cell yields are small, it is essential to bring the greatest analytic capacity to bear on each human tissue sample. The Immunophenotype Core (IPC) takes advantage of the ongoing experience of the Clynes and Sykes labs, in applying basic immunological research to translational questions in human diseases to now focus this expertise on inflammatory diseases of the skin. Flow cytometry has become the primary tool for the identification of cell populations according to specific parameters, and is therefore employed by an ever-growing number of biomedical scientists. The ability to design, perform and analyze data from multi-parametric flow cytometric experiments requires technical expertise but also immunological expertise to appropriately design the experiment. The method is technologically complex and the equipment is expensive, necessitating shared instrumentation amongst groups of users to facilitate expert use and availability of these vital and versatile instruments. In the next five years, together with our colleagues in the SDRCCUMC, flow cytometric analysis and sorting strategies will 1) define the inflammatory response in alopecia, specifically the NKG2D positive CDS T cell population in the human and mouse;2) isolate relevant cutaneous rare cellular populations for functional studies (Merkel, epithelial stem cell, myeloid suppressor cells, and 3) analyse phenotypic consequences of skin perturbations on cellular function.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR044535-12
Application #
8499259
Study Section
Special Emphasis Panel (ZAR1-HL)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
12
Fiscal Year
2013
Total Cost
$155,356
Indirect Cost
$58,860
Name
Columbia University (N.Y.)
Department
Type
DUNS #
621889815
City
New York
State
NY
Country
United States
Zip Code
10032
Shen, Yao; Stanislauskas, Milda; Li, Gen et al. (2017) Epigenetic and genetic dissections of UV-induced global gene dysregulation in skin cells through multi-omics analyses. Sci Rep 7:42646
Mackay-Wiggan, Julian; Jabbari, Ali; Nguyen, Nhan et al. (2016) Oral ruxolitinib induces hair regrowth in patients with moderate-to-severe alopecia areata. JCI Insight 1:e89790
Shen, Yao; Kim, Arianna L; Du, Rong et al. (2016) Transcriptome Analysis Identifies the Dysregulation of Ultraviolet Target Genes in Human Skin Cancers. PLoS One 11:e0163054
Dai, Zhenpeng; Xing, Luzhou; Cerise, Jane et al. (2016) CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata. J Immunol 197:1089-99
Mathew, Grinu; Hannan, Abdul; Hertzler-Schaefer, Kristina et al. (2016) Targeting of Ras-mediated FGF signaling suppresses Pten-deficient skin tumor. Proc Natl Acad Sci U S A 113:13156-13161
Dainichi, Teruki; Hayden, Matthew S; Park, Sung-Gyoo et al. (2016) PDK1 Is a Regulator of Epidermal Differentiation that Activates and Organizes Asymmetric Cell Division. Cell Rep 15:1615-23
Johnson, Dylan; Mathur, Mohit C; Kobayashi, Tomoyoshi et al. (2016) The Cardiomyopathy Mutation, R146G Troponin I, Stabilizes the Intermediate ""C"" State of Regulated Actin under High- and Low-Free Ca(2+) Conditions. Biochemistry 55:4533-40
Abaci, Hasan E; Guo, Zongyou; Coffman, Abigail et al. (2016) Human Skin Constructs with Spatially Controlled Vasculature Using Primary and iPSC-Derived Endothelial Cells. Adv Healthc Mater 5:1800-7
Shinkuma, Satoru; Guo, Zongyou; Christiano, Angela M (2016) Site-specific genome editing for correction of induced pluripotent stem cells derived from dominant dystrophic epidermolysis bullosa. Proc Natl Acad Sci U S A 113:5676-81
Sun, Xiaoyun; Kim, Arianna; Nakatani, Masashi et al. (2016) Distinctive molecular responses to ultraviolet radiation between keratinocytes and melanocytes. Exp Dermatol 25:708-13

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