The Molecular Core of the YCCMD provides support for a number of specialized methodologies and technical assistance in essential molecular techniques. The creation of animal models of gene dysregulation and the characterization of target gene expression is fully supported the Molecular Core, with further analysis both in vivo and in vitro provided by the Cell and Physiology Cores.
Aim 1. To provide as services, a) the construction of transgenes and gene disruption vectors, and b) the identification of genetically altered or naturally occurring mutant mice. This includes constitutive transgenes, inducible transgenes using the tetracycline transactivator system, conventional gene ablation vectors, conditional gene targeting vectors (using Cre-loxP or Flp-frf approaches), gene knock-in with expression tags (IRES-LacZ or -GFP) and inducible gene targeting (with Cre-ER or Cre-PR fusions). Genotyping is carried out by PCR and/or Southern blot analysis of tail DMA and/or ES cell DMA.
Aim 2. To provide as services, a) the localization of gene expression by in situ hybridization, and b) the quantitation of gene expression by quantitative, real-time RT-PCR. For expression localization, this includes the dissection and fixation of tissues, embedding in paraffin or OCT, sectioning by microtome or cryostat, preparation of riboprobes or oligoprobes, hybridization, emulsion autoradiography and image analysis. For quantitation of gene expression, this includes tissue dissection, RNA preparation, RT primer selection and PCR primer design.
Aim 3. To provide technical assistance, training and resources for essential molecular methods, including: RNA and genomic DNA preparation and analysis;stable or transient cell transfection; reporter gene analysis;PCR-related techniques, such as mutagenesis, cloning, overlap extension and inverse PCR;siRNA design, Dicer pools and shRNA (short hairpin) vectors and viruses for projects that require the suppression of gene expression by RNA interference;and BAG recombineering.

Public Health Relevance

The mission of the Molecular Core is to support the creation and analysis of animal models relevant to musculoskeletal disease and to facilitate the introduction and use of a range of molecular genetic methods by member investigators.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Center Core Grants (P30)
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Special Emphasis Panel (ZAR1-CHW-G)
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Yale University
New Haven
United States
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McCarthy, Thomas L; Yun, Zhong; Madri, Joseph A et al. (2014) Stratified control of IGF-I expression by hypoxia and stress hormones in osteoblasts. Gene 539:141-51
Wang, Meina; VanHouten, Joshua N; Nasiri, Ali R et al. (2014) Periosteal PTHrP regulates cortical bone modeling during linear growth in mice. J Anat 225:71-82
Wang, Meina; Nasiri, Ali; VanHouten, Joshua N et al. (2014) The remarkable migration of the medial collateral ligament. J Anat 224:490-8
Scheller, Erica L; Troiano, Nancy; Vanhoutan, Joshua N et al. (2014) Use of osmium tetroxide staining with microcomputerized tomography to visualize and quantify bone marrow adipose tissue in vivo. Methods Enzymol 537:123-39
Mis, Emily K; Liem Jr, Karel F; Kong, Yong et al. (2014) Forward genetics defines Xylt1 as a key, conserved regulator of early chondrocyte maturation and skeletal length. Dev Biol 385:67-82
Juneja, Subhash C; Vonica, Alin; Zeiss, Caroline et al. (2014) Deletion of Mecom in mouse results in early-onset spinal deformity and osteopenia. Bone 60:148-61
Gao, Hui; Mejhert, Niklas; Fretz, Jackie A et al. (2014) Early B cell factor 1 regulates adipocyte morphology and lipolysis in white adipose tissue. Cell Metab 19:981-92
Yao, Chen; Yao, Gang-Qing; Sun, Ben-Hua et al. (2014) The transcription factor T-box 3 regulates colony-stimulating factor 1-dependent Jun dimerization protein 2 expression and plays an important role in osteoclastogenesis. J Biol Chem 289:6775-90
Gattu, Arijeet K; Birkenfeld, Andreas L; Iwakiri, Yasuko et al. (2014) Pigment epithelium-derived factor (PEDF) suppresses IL-1?-mediated c-Jun N-terminal kinase (JNK) activation to improve hepatocyte insulin signaling. Endocrinology 155:1373-85
Kacena, Melissa A; Gundberg, Caren M; Kacena 3rd, William J et al. (2013) The effects of GATA-1 and NF-E2 deficiency on bone biomechanical, biochemical, and mineral properties. J Cell Physiol 228:1594-600

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